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The map label for this gene is gltJ

Identifier: 218699019

GI number: 218699019

Start: 656060

End: 656800

Strand: Reverse

Name: gltJ

Synonym: ECIAI39_0622

Alternate gene names: 218699019

Gene position: 656800-656060 (Counterclockwise)

Preceding gene: 218699020

Following gene: 218699018

Centisome position: 12.8

GC content: 53.04

Gene sequence:

>741_bases
ATGTCTATAGACTGGAACTGGGGTATTTTTTTACAACAAGCCCCGTTCGGCAACACCACCTATCTTGGTTGGATCTGGAG
TGGTTTTCAGGTGACGATCGCTTTGTCGATCTGCGCCTGGATTATCGCTTTCCTCGTCGGCTCGTTATTCGGTATTTTAC
GTACCGTTCCAAACCGTTTTCTCTCCGGTCTGGGTACGTTGTATGTCGAACTGTTCCGCAACGTGCCGTTGATTGTGCAA
TTCTTTACCTGGTATCTGGTGATCCCGGAGCTGCTGCCAGAGAAAATCGGCATGTGGTTTAAGGCCGAGCTGGATCCCAA
TATTCAGTTTTTCCTTTCATCCATGCTCTGCCTGGGGCTGTTTACCGCCGCCCGTGTTTGCGAACAGGTACGCGCCGCTA
TTCAGTCGCTGCCGCGTGGGCAAAAGAATGCCGCGCTGGCAATGGGGCTGACACTGCCGCAGGCTTACCGCTACGTGCTG
TTGCCTAACGCGTATCGCGTTATCGTCCCGCCGATGACCTCCGAGATGATGAACCTGGTGAAAAACTCCGCCATCGCCTC
GACTATCGGTCTGGTAGATATGGCGGCGCAGGCGGGTAAATTACTCGATTACTCCGCTCACGCCTGGGAGTCGTTTACCG
CCATTACGCTTGCGTATGTGTTGATTAACGCTTTTATCATGCTGGTGATGACGCTGGTTGAACGTAAAGTTCGCCTGCCC
GGCAACATGGGGGGCAAATAA

Upstream 100 bases:

>100_bases
ATGGTCGTTCCCCATCGGGCCTGAAAACCGCAATACGCTGGGTAACAATCTTCGAGGGTAGCAGTTAACGCTGCTACCCT
CTTTTTTCTGGAGTAGATTT

Downstream 100 bases:

>100_bases
TGTACGAGTTTGACTGGAGTTCCATTGTCCCTTCCCTGCCATATCTGCTGGATGGGCTGGTGATCACCCTGAAAATCACC
GTCACGGCAGTGGTGATTGG

Product: glutamate and aspartate ABC transporter membrane protein

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSLFGILRTVPNRFLSGLGTLYVELFRNVPLIVQ
FFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP
GNMGGK

Sequences:

>Translated_246_residues
MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSLFGILRTVPNRFLSGLGTLYVELFRNVPLIVQ
FFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP
GNMGGK
>Mature_245_residues
SIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSLFGILRTVPNRFLSGLGTLYVELFRNVPLIVQF
FTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLL
PNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLPG
NMGGK

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=246, Percent_Identity=99.5934959349593, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1788226, Length=198, Percent_Identity=32.8282828282828, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI48994930, Length=123, Percent_Identity=39.8373983739837, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786873, Length=215, Percent_Identity=26.046511627907, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787030, Length=213, Percent_Identity=23.943661971831, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1788645, Length=217, Percent_Identity=27.1889400921659, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787086, Length=218, Percent_Identity=26.605504587156, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788646, Length=221, Percent_Identity=28.5067873303167, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082239, Length=169, Percent_Identity=26.0355029585799, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27469; Mature: 27338

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSLFGILRTVPNRF
CCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
FTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLLPNAYRVIVPPMTSEMMNLV
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHEEECCCCCEEEECCHHHHHHHHH
KNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP
HHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNMGGK
CCCCCC
>Mature Secondary Structure 
SIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSLFGILRTVPNRF
CCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
FTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLLPNAYRVIVPPMTSEMMNLV
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHEEECCCCCEEEECCHHHHHHHHH
KNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP
HHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNMGGK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]