Click here to switch to the map view.

The map label for this gene is mrdB

Identifier: 218699006

GI number: 218699006

Start: 643264

End: 644376

Strand: Reverse

Name: mrdB

Synonym: ECIAI39_0609

Alternate gene names: 218699006

Gene position: 644376-643264 (Counterclockwise)

Preceding gene: 218699007

Following gene: 218699005

Centisome position: 12.56

GC content: 51.21

Gene sequence:

>1113_bases
ATGACGGATAATCCGAATAAAAAAACATTCTGGGATAAAGTCCATCTCGATCCCACAATGCTGCTGATTTTACTGGCATT
GCTGGTTTACAGCGCCCTGGTTATCTGGAGCGCCAGCGGTCAGGATATTGGCATGATGGAGCGTAAAATCGGCCAAATCG
CGATGGGTCTGGTCATCATGGTGGTGATGGCGCAAATTCCTCCACGCGTTTATGAAGGCTGGGCCCCCTATCTCTATATC
ATCTGTATTATTTTGCTGGTGGCGGTAGATGCTTTCGGTGCCATCTCTAAAGGCGCTCAACGCTGGCTGGACCTCGGTAT
TGTTCGTTTTCAACCGTCGGAAATTGCCAAGATAGCCGTACCGTTGATGGTTGCACGCTTTATCAACCGCGACGTTTGCC
CACCTTCATTGAAGAATACAGCTATCGCGCTGGTACTGATATTTATGCCCACGCTGCTGGTGGCTGCACAGCCTGACCTG
GGAACATCAATCCTCGTTGCGCTTTCCGGCCTGTTTGTACTGTTCCTCTCTGGCCTTAGCTGGCGTCTGATTGGCGTCGC
AGTAGTGCTGGTAGCGGCGTTCATTCCGATTCTGTGGTTCTTCCTGATGCATGATTACCAGCGCCAGCGCGTAATGATGC
TCCTGGACCCGGAATCAGACCCACTCGGCGCGGGCTATCATATTATTCAATCTAAAATTGCTATTGGCTCCGGCGGATTA
CGCGGCAAAGGCTGGCTGCACGGTACTCAGTCACAGCTTGAATTTCTCCCCGAACGCCATACCGACTTTATCTTCGCGGT
ACTGGCGGAAGAGCTGGGATTAGTGGGCATTTTGATTCTGCTCGCTCTTTACATTCTGCTGATCATGCGCGGGCTGTGGA
TAGCCGCCAGAGCGCAAACCACCTTTGGTCGCGTCATGGCTGGCGGCTTAATGCTGATATTATTCGTTTATGTCTTCGTA
AATATTGGTATGGTAAGCGGTATTCTGCCGGTTGTAGGGGTTCCGCTCCCACTGGTCAGTTATGGAGGATCGGCGCTAAT
TGTGCTGATGGCTGGGTTCGGGATTGTAATGTCAATCCACACCCACAGGAAAATGTTGTCGAAAAGCGTGTAA

Upstream 100 bases:

>100_bases
CGCTGATGCGCCAGATCCTCGACCACATTATGCTGGGTGATAACAACACCGATCTGCCTGCGGAAAATCCAGCGGTTGCC
GCAGCGGAGGACCATTAATC

Downstream 100 bases:

>100_bases
GAGGTGCGCAATGCGTAAGCAGTGGCTCGGGATCTGCATCGCGGCAGGAATGCTCGCGGCATGTACAAGCGATGATGGTC
AGCAACAGACGGTAAGTGTA

Product: cell wall shape-determining protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYI
ICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV

Sequences:

>Translated_370_residues
MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYI
ICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV
>Mature_369_residues
TDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYII
CIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDLG
TSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGLR
GKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVN
IGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV

Specific function: This is one of the proteins responsible for the rod shape of E.coli. Is required for the expression of the enzymatic activity of PBP2, which is thought to synthesize peptidoglycan in the step of initiation of cell elongation [H]

COG id: COG0772

COG function: function code D; Bacterial cell division membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ftsW/rodA/spoVE family [H]

Homologues:

Organism=Escherichia coli, GI1786853, Length=370, Percent_Identity=99.7297297297297, Blast_Score=728, Evalue=0.0,
Organism=Escherichia coli, GI1786277, Length=317, Percent_Identity=31.8611987381703, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001182
- InterPro:   IPR018365
- InterPro:   IPR011923 [H]

Pfam domain/function: PF01098 FTSW_RODA_SPOVE [H]

EC number: NA

Molecular weight: Translated: 40491; Mature: 40360

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00428 FTSW_RODA_SPOVE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVIM
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAV
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
PLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDLGTSILVALSGLFVLFLSGLS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCH
WRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQT
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKSV
HHHHHHHCCC
>Mature Secondary Structure 
TDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVIM
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAV
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
PLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDLGTSILVALSGLFVLFLSGLS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCH
WRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPESDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQT
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKSV
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]