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The map label for this gene is yaeJ

Identifier: 218698859

GI number: 218698859

Start: 494370

End: 494792

Strand: Reverse

Name: yaeJ

Synonym: ECIAI39_0456

Alternate gene names: 218698859

Gene position: 494792-494370 (Counterclockwise)

Preceding gene: 218698860

Following gene: 218698858

Centisome position: 9.64

GC content: 54.61

Gene sequence:

>423_bases
ATGATTGTGATTTCCCGACATGTTGCTATCCCTGATGGCGAGTTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGG
GCAGCATGTTAATAAGACCTCAACGGCTATTCATCTGCGTTTTGACATTCGGGCGTCCAGCCTGCCAGAGTATTACAAAG
AGCGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGACGGAGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAG
GAACTGAACCGCGAAGCGGCGCTGGCCCGGCTGGTGGCAGTGATTAAAGATTTAACAACAGAACAAAAAGCCCGACGACC
TACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCA
AAGTGCGCAGCGATCGGGAATAA

Upstream 100 bases:

>100_bases
GTACCATGACGCTGCAGGCAACGATTCAGGATGGTGTTATCTGGTTATCGGATGATAAGAATAATCTGGAAGTGAACTTA
ACCGTCTGGCAACAACCTTC

Downstream 100 bases:

>100_bases
AAAGAAGGAATGGATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACTCTGATGGGATGTAATA
ATCGGGCCGAAGTCGACACG

Product: peptidyl-tRNA hydrolase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIRASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQ
ELNREAALARLVAVIKDLTTEQKARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSDRE

Sequences:

>Translated_140_residues
MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIRASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQ
ELNREAALARLVAVIKDLTTEQKARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSDRE
>Mature_140_residues
MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIRASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQ
ELNREAALARLVAVIKDLTTEQKARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSDRE

Specific function: Unknown

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Escherichia coli, GI1786389, Length=140, Percent_Identity=96.4285714285714, Blast_Score=270, Evalue=2e-74,
Organism=Drosophila melanogaster, GI19921098, Length=136, Percent_Identity=34.5588235294118, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 15669; Mature: 15669

Theoretical pI: Translated: 11.24; Mature: 11.24

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIRASSLPEYYKERLLAAS
CEEEECEEECCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
HHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQKARRPTRPTRASKERRLA
HHEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
SKAQKSSVKAMRGKVRSDRE
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIRASSLPEYYKERLLAAS
CEEEECEEECCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
HHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQKARRPTRPTRASKERRLA
HHEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
SKAQKSSVKAMRGKVRSDRE
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7635807; 9278503; 7635808 [H]