Click here to switch to the map view.

The map label for this gene is yafE

Identifier: 218698843

GI number: 218698843

Start: 473506

End: 474276

Strand: Reverse

Name: yafE

Synonym: ECIAI39_0439

Alternate gene names: 218698843

Gene position: 474276-473506 (Counterclockwise)

Preceding gene: 218698844

Following gene: 218698840

Centisome position: 9.24

GC content: 49.55

Gene sequence:

>771_bases
ATGACAACACAATCCCACCATGACCACGTAGAAAAGCAGTTTAGCTCGCAGGCCAGTGAGTATTTAACCAGTACCGTACA
TGCATCCGGGCGAGATTTACAGCGCCTGGCGGTGCGTCTGGCTGATTATCCTGATGCAAGTGTACTTGATATGGGCTGCG
GAGCAGGGCATGCCAGCTTTGTCGCTGCGCAGAACGTGAGCGCGGTGGTGGCGTATGACTTATCTGCCCAAATGCTGGAT
GTCGTGGCACAAGCTGCCGAAGCCCGGCAACTGAAAAATATCACCACCCGCCAGGGTTATGCCGAAAGTCTGCCATTTGC
CGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGTGAAGTGA
ATCGGGTATTGGAACCCGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCATCCAGTGCGCGACATCTGGTTA
CAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCAGCGGTGAGTGGTTGAAGTTAATCAATGAAGC
CAATCTGATAGTTGATAATTTAATTACAGATAAGTTACCGCTGGAATTTTCTTCATGGGTAGCGAGAATGCGTACGCCAG
AAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAAAACGTATTTTGCCTTGCAGAATGAT
GGCTCTTTCACCAGTGATATCATCATGCTAGAAGCACATAAAGCGGCATAA

Upstream 100 bases:

>100_bases
AGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCCCTTTTTAATCA
AACAATAAAAGGACAGCGCA

Downstream 100 bases:

>100_bases
ATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTTATTATCTGGTTTGTCAGGAGTCTGGCATGCTGTTGTTTTTCACAA
ACAACGTCAGCTTATCGCCT

Product: putative S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYPDASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLD
VVAQAAEARQLKNITTRQGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLEPGGRLIVMDVMSPGHPVRDIWL
QTVEALRDTSHVRNYASGEWLKLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVDAIRIYQQSASTEVKTYFALQND
GSFTSDIIMLEAHKAA

Sequences:

>Translated_256_residues
MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYPDASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLD
VVAQAAEARQLKNITTRQGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLEPGGRLIVMDVMSPGHPVRDIWL
QTVEALRDTSHVRNYASGEWLKLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVDAIRIYQQSASTEVKTYFALQND
GSFTSDIIMLEAHKAA
>Mature_255_residues
TTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYPDASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDV
VAQAAEARQLKNITTRQGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLEPGGRLIVMDVMSPGHPVRDIWLQ
TVEALRDTSHVRNYASGEWLKLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVDAIRIYQQSASTEVKTYFALQNDG
SFTSDIIMLEAHKAA

Specific function: Unknown

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786404, Length=205, Percent_Identity=92.6829268292683, Blast_Score=393, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: NA

Molecular weight: Translated: 28307; Mature: 28176

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYPDASVLDMGCGAGHASF
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCCHHHH
VAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAESLPFADNAFDIVISRYSA
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHCC
HHWHDVGAALREVNRVLEPGGRLIVMDVMSPGHPVRDIWLQTVEALRDTSHVRNYASGEW
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
LKLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVDAIRIYQQSASTEVKTYFALQND
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEECC
GSFTSDIIMLEAHKAA
CCCCCCEEEEEECCCC
>Mature Secondary Structure 
TTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYPDASVLDMGCGAGHASF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCCCHHHH
VAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAESLPFADNAFDIVISRYSA
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHCC
HHWHDVGAALREVNRVLEPGGRLIVMDVMSPGHPVRDIWLQTVEALRDTSHVRNYASGEW
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
LKLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVDAIRIYQQSASTEVKTYFALQND
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEECC
GSFTSDIIMLEAHKAA
CCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1663890; 9278503 [H]