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The map label for this gene is yafL

Identifier: 218698827

GI number: 218698827

Start: 459740

End: 460489

Strand: Reverse

Name: yafL

Synonym: ECIAI39_0423

Alternate gene names: 218698827

Gene position: 460489-459740 (Counterclockwise)

Preceding gene: 218698833

Following gene: 218698826

Centisome position: 8.97

GC content: 52.93

Gene sequence:

>750_bases
ATGTCCTTGCCGTCGATTCCCTCTTTTGTGTTGTCGGGATTATTGTTGCTTTGCTTGCCGTTTTCTTCATTTGCCAGCGC
CACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAA
CTCATCTGAAAAAGCAGGCCGGCTATATTGTGGAAGGCAATGCCGAAAGCAGAAAGGCGCTGCGCCAGCACAACCGCGAA
CAGATAAAACAGCATCCAGAATGGTTTCCTGCGCCGCTCAAGGCGAGTGACAGACGCTGGCAGGCGCTGGCGGAGAACAA
CCATTTCTTAAGCAGCGACCATCTACACAACATCACCGAAGTGGCGATTCACCGCCTGGAGCAGCAGCTTGGCAAGCCTT
ATGTCTGGGGCGGCACGCGGCCCGATAAAGGCTTCGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAG
GCGAAGCTCCCGCGCACAGCCAATGAGATGTATCACTACCATCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGG
AGATTTGCTGTTTTTCCATATTCACAGCCGCGAGATAGCCGATCATATGGGGGTGTATCTGGGAGACGGTCAGTTTATCG
AGTCACCACGTACCGGCGAAACCATTCGGATAAGCCGGTTAGCTGAACCTTTCTGGCAGGATCATTTTTTAGGCGCGCGG
CGGATTTTGACGGAAGAGACGATTTTGTAG

Upstream 100 bases:

>100_bases
GAAATATATTTATTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGGTACGGGCCGGGATACACTGCAACATCAATGC
ATGACCAGCCATTATTGAGT

Downstream 100 bases:

>100_bases
GACGGAGAGGGCGTTCACGCCGGATTCGGCAATTGTGCACCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAC
AAATCTGTGCGTTTATGCGA

Product: putative exported hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQAGYIVEGNAESRKALRQHNRE
QIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRISRLAEPFWQDHFLGAR
RILTEETIL

Sequences:

>Translated_249_residues
MSLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQAGYIVEGNAESRKALRQHNRE
QIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRISRLAEPFWQDHFLGAR
RILTEETIL
>Mature_248_residues
SLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQAGYIVEGNAESRKALRQHNREQ
IKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILEA
KLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRISRLAEPFWQDHFLGARR
ILTEETIL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=249, Percent_Identity=97.9919678714859, Blast_Score=512, Evalue=1e-147,
Organism=Escherichia coli, GI1787944, Length=134, Percent_Identity=52.2388059701493, Blast_Score=176, Evalue=9e-46,
Organism=Escherichia coli, GI1788501, Length=114, Percent_Identity=34.2105263157895, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 28736; Mature: 28605

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQA
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYIVEGNAESRKALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITE
CEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
VAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEMYHYHRATIVA
HHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEE
NNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRISRLAEPFWQDHFLGAR
CCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHH
RILTEETIL
HHHHHHCCC
>Mature Secondary Structure 
SLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQA
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYIVEGNAESRKALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITE
CEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
VAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEMYHYHRATIVA
HHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEE
NNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRISRLAEPFWQDHFLGAR
CCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHH
RILTEETIL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]