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The map label for this gene is dinB

Identifier: 218698823

GI number: 218698823

Start: 455360

End: 456415

Strand: Reverse

Name: dinB

Synonym: ECIAI39_0418

Alternate gene names: 218698823

Gene position: 456415-455360 (Counterclockwise)

Preceding gene: 218698824

Following gene: 218698822

Centisome position: 8.89

GC content: 53.41

Gene sequence:

>1056_bases
ATGCGTAAAATCATTCATGTGGATATGGACTGCTTTTTCGCAGCGGTGGAGATGCGCGACAATCCTGCCCTGCGCGATAT
CCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTAC
GCAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACATCTCACGCTGCTTCCAGGGCGCTTTGACGCCTACAAA
GAAGCCTCAAATCATATCCGCGAAATTTTCTCGCGCTATACCTCGCGCATTGAACCGTTATCACTGGATGAAGCCTATCT
CGACGTCACCGATAGTGTGCATTGCCACGGCTCCGCGACCCTCATCGCCCAGGAAATCCGCCAGACGATCTTTAACGAGC
TGCAACTGACGGCATCTGCGGGCGTTGCGCCGGTAAAGTTTCTCGCCAAAATTGCTTCCGACATGAATAAACCCAACGGT
CAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTCTGCAAACCTTACCGCTGGCAAAAATTCCCGGCGTCGGCAAAGT
CTCGGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGGTGATGTGCAAAAGTGTGATCTGGTGACTCTGCTCAAAC
GTTTTGGCAAATTTGGACGCATTTTGTGGGAGCGTAGTCAGGGGATTGACGAGCGCGACGTTAACAGCGAACGGTTGCGA
AAATCCGTCGGCGTGGAACGCACGATGGCGGAAGATATTCATCACTGGTCTGAATGTGAAGCGATTATTGAGCGGCTGTA
TCCTGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTTGATG
ATTTTCAGCAAACCACTCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGACTTAATCGCCACCGCGCGTAAAACCTGG
AATGAACGTCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTACTTGACCCTCAAATGGAAAGACAACT
GGTGCTGGGATTATGA

Upstream 100 bases:

>100_bases
CAGCAGGTGCTTTCACAGCGAACGCGTTAAATGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCACTGTATACTTTA
CCAGTGTTGAGAGGTGAGCA

Downstream 100 bases:

>100_bases
ATAACATACAAATAAGAAACTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACC
GCCAGTCAGCATTATTCACC

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK
EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTW
NERRGGRGVRLVGLHVTLLDPQMERQLVLGL

Sequences:

>Translated_351_residues
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK
EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTW
NERRGGRGVRLVGLHVTLLDPQMERQLVLGL
>Mature_351_residues
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYK
EASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTW
NERRGGRGVRLVGLHVTLLDPQMERQLVLGL

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI7705344, Length=356, Percent_Identity=33.4269662921348, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI84043967, Length=324, Percent_Identity=31.1728395061728, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI7706681, Length=325, Percent_Identity=31.3846153846154, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI154350220, Length=251, Percent_Identity=32.6693227091633, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI5729982, Length=359, Percent_Identity=30.3621169916435, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786425, Length=351, Percent_Identity=99.4301994301994, Blast_Score=720, Evalue=0.0,
Organism=Escherichia coli, GI1787432, Length=217, Percent_Identity=26.2672811059908, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI193205700, Length=406, Percent_Identity=32.0197044334975, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17537959, Length=290, Percent_Identity=29.6551724137931, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI193205702, Length=351, Percent_Identity=27.6353276353276, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115534089, Length=163, Percent_Identity=34.3558282208589, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324921, Length=208, Percent_Identity=29.8076923076923, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19923006, Length=327, Percent_Identity=31.8042813455657, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21355641, Length=356, Percent_Identity=29.2134831460674, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24644984, Length=356, Percent_Identity=29.2134831460674, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24668444, Length=121, Percent_Identity=35.5371900826446, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39485; Mature: 39485

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTG
CCCEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEECCCCCHHHHCCHHCCCCC
MALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSAT
CHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCHH
LIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAEVPAFLQTLPLAK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCHHH
IPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKFGRILWERSQGIDERDVNSERLR
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHH
EHVWPRLNKADLIATARKTWNERRGGRGVRLVGLHVTLLDPQMERQLVLGL
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHCCC
>Mature Secondary Structure
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTG
CCCEEEECHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEECCCCCHHHHCCHHCCCCC
MALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSAT
CHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCHH
LIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAEVPAFLQTLPLAK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCHHH
IPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKFGRILWERSQGIDERDVNSERLR
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHH
EHVWPRLNKADLIATARKTWNERRGGRGVRLVGLHVTLLDPQMERQLVLGL
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA