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The map label for this gene is prfH

Identifier: 218698820

GI number: 218698820

Start: 452970

End: 453470

Strand: Reverse

Name: prfH

Synonym: ECIAI39_0415

Alternate gene names: 218698820

Gene position: 453470-452970 (Counterclockwise)

Preceding gene: 218698821

Following gene: 218698818

Centisome position: 8.84

GC content: 54.49

Gene sequence:

>501_bases
GTGCTGGAAACAGAAACGGGTCGCTACTCTGACACACTGCGTTCGGCGCTGATTTCTCTGGATGGCGATAACGCATGGGC
TCTAAGCGAAAGCTGGTGTGGCACTATCCAGTGGATTTGTCCGAGTCCGTATCGGCCTCATCATGGGCGCAAAAACTGGT
TTCTGGGCATTGGGCGTTTTACCGCTGATGAGCAGGAACAATCCGACGCGATCCGTTATGAGACGCTGCGTTCGTCGGGG
CCGGGCGGTCAACATGTCAATAATACCGACTCGGCGGTACGCGCCACGCATCTGGCATCCGGTATTAGCGTGAAGGTTCA
GTCAGAGCGCAGTCAGCATGCTAACAAACGGCTGGCGCGATTACTGATTGCCTGGAAGCTGGAACAACAGCAACAGGAAA
ATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGAGACGGACATTTACAGGG
ATGGCTTTTATCGAAGGATAA

Upstream 100 bases:

>100_bases
ACTCTCCTCAGCTCAGGGGCCGGAAGAATGTTGCCTGGCAGTGAAAAAGGCGCTGGACAGGTTGATTAAAGAAGCTACCC
GACAAGACGTCGCGGTAACG

Downstream 100 bases:

>100_bases
TGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAG
CAGTTCAGTCAGCAGTGTCC

Product: peptide chain release factor-like protein

Products: NA

Alternate protein names: RF-H [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSG
PGGQHVNNTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTG
MAFIEG

Sequences:

>Translated_166_residues
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSG
PGGQHVNNTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTG
MAFIEG
>Mature_166_residues
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSG
PGGQHVNNTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTG
MAFIEG

Specific function: Could be involved in translational termination [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Escherichia coli, GI1787462, Length=93, Percent_Identity=45.1612903225806, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367172, Length=133, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321295, Length=57, Percent_Identity=57.8947368421053, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921226, Length=69, Percent_Identity=53.6231884057971, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352
- InterPro:   IPR017509 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 18834; Mature: 18834

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRF
CCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCCEEEEECCC
TADEQEQSDAIRYETLRSSGPGGQHVNNTDSAVRATHLASGISVKVQSERSQHANKRLAR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHH
LLIAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRF
CCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCCEEEEECCC
TADEQEQSDAIRYETLRSSGPGGQHVNNTDSAVRATHLASGISVKVQSERSQHANKRLAR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHH
LLIAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1695895; 9278503; 1408743 [H]