Click here to switch to the map view.

The map label for this gene is yahA

Identifier: 218698781

GI number: 218698781

Start: 404209

End: 405297

Strand: Reverse

Name: yahA

Synonym: ECIAI39_0371

Alternate gene names: 218698781

Gene position: 405297-404209 (Counterclockwise)

Preceding gene: 218698782

Following gene: 218698778

Centisome position: 7.9

GC content: 45.91

Gene sequence:

>1089_bases
ATGAATTCATGTGATTTTCGTGTTTTTCTGCAAGAGTTCGGTACAACGGTTCATTTGTCATTGCCTGGTAGCGTATCCGA
GAAAGAACGACTGCTACTCAAGCTGCTGATGCAGGGAATGTCTGTAACAGAAATATCACAGTACAGAAATCGCAGTGCAA
AGACCATTTCACATCAAAAGAAACAGCTCTTTGAGAAACTGGGGATTCAGAGCGATATTACTTTCTGGCGGGATATTTTC
TTTCAGTACAATCCGGAGATCATATCCGCCACGGGTAATAATAGTCACAAATATATTAATGATAATCACTATCACCATAT
CGTCACGCCTGAAGCCATCAGTCTGGCGTTGGAAAACCATGAATTTAAACCGTGGATCCAACCGGTTTTCTGCGCGCAGA
CTGGGGTACTGACGGGCTGTGAGGTGCTTGTTCGCTGGGAACATCCGCAAACGGGAATCATCCCGCCGGATCAATTTATT
CCTCTGGCGGAATCATCTGGTCTTATCGTCATAATGACCCGCCAGTTGATGAAACAGACTGCGGATATTCTGATGCCGGT
AAAACATTTGCTGCCGGACAACTTCCATATAGGTATCAACGTCTCGGCGGGCTGTTTTTTGGCTGCGGGATTTGAAAAAG
AGTGTCTGAACCTGGTTAAGAAATTAGGTAACGATAAAATCAAGCTGGTTCTTGAGCTGACGGAACGTAACCCTATTCCG
GTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGG
TTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAAATGGCGAGCG
TGGACGAAATATCCGGTCATATTGTGGACAATATTGTCGAACTGGCGCGTAAGCCTGGTCTGAGTATCGTGGCGGAAGGG
GTAGAAACCCAGGAGCAGGCGGATTTAATGATCGGCAAAGGCGTTCACTTTTTGCAGGGCTATTTGTACTCTCCGCCAGT
ACCGGGTAATAAATTTATCTCTGAATGGGTAATGAAAGCAGGTGGTTGA

Upstream 100 bases:

>100_bases
GTATGGCATCGCTAATGTTTATGGTTACCCCTTATGTGTGCTCAGGAATCCACAGGTAATCACTCATACTGAACAGCGAT
AAAAGATAAAAGGTGTGTTC

Downstream 100 bases:

>100_bases
TGTAAACCGCTATTCACAGCGCATCGGGAGGTTGGCAGCGATTAATTCTCCCGATGCAGTAAATCCTGATAGATCCCCGT
TAATATGCCATTTGGCGAAA

Product: cyclic di-GMP phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTEISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIF
FQYNPEIISATGNNSHKYINDNHYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIP
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEG
VETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKAGG

Sequences:

>Translated_362_residues
MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTEISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIF
FQYNPEIISATGNNSHKYINDNHYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIP
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEG
VETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKAGG
>Mature_362_residues
MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTEISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIF
FQYNPEIISATGNNSHKYINDNHYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIP
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEG
VETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKAGG

Specific function: Hydrolyzes c-di-GMP (cyclic bis(3'-5') dimeric GMP) to 5'-pGpG, known as PDE-A activity. PDE-B activity, that is hydrolysis of 5'-pGpG to GMP, proceeds only very slowly [H]

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786507, Length=362, Percent_Identity=99.171270718232, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI87081845, Length=251, Percent_Identity=44.2231075697211, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87081980, Length=247, Percent_Identity=39.2712550607287, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1788502, Length=247, Percent_Identity=36.8421052631579, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87081921, Length=330, Percent_Identity=30, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081743, Length=245, Percent_Identity=32.6530612244898, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI226510982, Length=243, Percent_Identity=33.7448559670782, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1790496, Length=242, Percent_Identity=31.8181818181818, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787541, Length=256, Percent_Identity=30.078125, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788849, Length=233, Percent_Identity=30.4721030042918, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI87082096, Length=210, Percent_Identity=32.3809523809524, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787055, Length=249, Percent_Identity=29.718875502008, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633
- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00563 EAL; PF00196 GerE [H]

EC number: NA

Molecular weight: Translated: 40739; Mature: 40739

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50043 HTH_LUXR_2 ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTEISQYRNRSAKTISHQK
CCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
KQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDNHYHHIVTPEAISLALENH
HHHHHHHCCCCCHHHHHHHHHEECCEEEEECCCCCCCCCCCCCEEEEECHHHHEEECCCC
EFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFIPLAESSGLIVIMTRQLMKQT
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
ADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIP
HHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHCCCEEEEEEEEECCCCCC
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASVDEISGH
CCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IVDNIVELARKPGLSIVAEGVETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHCEECCCCCCCHHHHHHHHHHC
GG
CC
>Mature Secondary Structure
MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTEISQYRNRSAKTISHQK
CCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
KQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDNHYHHIVTPEAISLALENH
HHHHHHHCCCCCHHHHHHHHHEECCEEEEECCCCCCCCCCCCCEEEEECHHHHEEECCCC
EFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFIPLAESSGLIVIMTRQLMKQT
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
ADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIP
HHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHCCCEEEEEEEEECCCCCC
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASVDEISGH
CCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IVDNIVELARKPGLSIVAEGVETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHCEECCCCCCCHHHHHHHHHHC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 1956285 [H]