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The map label for this gene is cynT

Identifier: 218698752

GI number: 218698752

Start: 372572

End: 373231

Strand: Reverse

Name: cynT

Synonym: ECIAI39_0339

Alternate gene names: 218698752

Gene position: 373231-372572 (Counterclockwise)

Preceding gene: 218698756

Following gene: 218698751

Centisome position: 7.27

GC content: 57.88

Gene sequence:

>660_bases
GTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAACAGCTGGC
GACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGC
CTGGCGATCTGTTCGTTATTCGCAACGCGGGCAATATCGTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCG
GTGGAGTACGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGCGGTCATTCCAACTGTGGCGCGATGACCGCCAT
TGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATG
AGGCGCGCCCGCATCCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTG
CAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGCAAGCGG
CAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCCGCTAATCCTCGCGTTTGTGCCATACCGCTAC
GCCAACCGACCGCAGCGTAA

Upstream 100 bases:

>100_bases
TATAAGTAAATCCAATAGAACTCATCATAAATGAGACTTTTACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCA
GAGACAGACAGAGGTTAACG

Downstream 100 bases:

>100_bases
CCTTATTTTTAAACCATCAGGAGATCCACCATGATTCAGTCGCAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGC
CATTTTGCTCAGCAAAGCTA

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSAS
VEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
QTHPSVRLALEEGRIALHGWVYDIASGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA

Sequences:

>Translated_219_residues
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSAS
VEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
QTHPSVRLALEEGRIALHGWVYDIASGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA
>Mature_219_residues
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSAS
VEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
QTHPSVRLALEEGRIALHGWVYDIASGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=219, Percent_Identity=99.0867579908676, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1786318, Length=176, Percent_Identity=32.3863636363636, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25147564, Length=237, Percent_Identity=29.1139240506329, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI115532990, Length=237, Percent_Identity=25.7383966244726, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324292, Length=178, Percent_Identity=30.3370786516854, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24645213, Length=245, Percent_Identity=22.0408163265306, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23716; Mature: 23716

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVI
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEE
RNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVS
ECCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHH
HWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANLQTHPSVRLALEEGRIALHGW
HHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEE
VYDIASGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA
EEECCCCCEEEECCCCCEEEEECCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVI
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEE
RNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVS
ECCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHH
HWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANLQTHPSVRLALEEGRIALHGW
HHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEE
VYDIASGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA
EEECCCCCEEEECCCCCEEEEECCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]