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The map label for this gene is aroL

Identifier: 218698707

GI number: 218698707

Start: 327067

End: 327591

Strand: Reverse

Name: aroL

Synonym: ECIAI39_0293

Alternate gene names: 218698707

Gene position: 327591-327067 (Counterclockwise)

Preceding gene: 218698708

Following gene: 218698706

Centisome position: 6.38

GC content: 51.62

Gene sequence:

>525_bases
ATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAA
CCGTCGGTTTGTTGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTTGCGGAGATCGTCGAAAGGGAAGAGT
GGACGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTGCGCCATCCACCGTTATCGCCACGGGCGGCGGC
ATTATTCTGACGGAATTTAATCGTCACTTTATGCAAAATAACGGGATCGTGGTTTATTTGTGTGCGCCAGTATCAGTCCT
GGTTAACCGACTGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGGAAACCACTGAGCGAAGAAGTTCAGG
AAGTGCTGGAAGAACGCGATGCGCTTTATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTG
ATTTCTGAAATTCGCAGTGCCCTGGCACAGACGATCAATTGTTGA

Upstream 100 bases:

>100_bases
TTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGA
CCTATTGGGGAAAACCCACG

Downstream 100 bases:

>100_bases
TTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAG
CATATATAGTGACGATTGAA

Product: shikimate kinase II

Products: NA

Alternate protein names: SK 2 [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWTGFRARETAALEAVTAPSTVIATGGG
IILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
ISEIRSALAQTINC

Sequences:

>Translated_174_residues
MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWTGFRARETAALEAVTAPSTVIATGGG
IILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
ISEIRSALAQTINC
>Mature_173_residues
TQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWTGFRARETAALEAVTAPSTVIATGGGI
ILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVI
SEIRSALAQTINC

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate [H]

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family. AroL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786587, Length=174, Percent_Identity=99.4252873563218, Blast_Score=355, Evalue=1e-99,
Organism=Escherichia coli, GI87082255, Length=160, Percent_Identity=33.75, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000623
- InterPro:   IPR023000 [H]

Pfam domain/function: PF01202 SKI [H]

EC number: =2.7.1.71 [H]

Molecular weight: Translated: 19181; Mature: 19050

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWTGFRAR
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCHH
ETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLR
HHHHHHHHCCCCEEEECCCCEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCC
PTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVISEIRSALAQTINC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWTGFRAR
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCHH
ETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLR
HHHHHHHHCCCCEEEECCCCEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCC
PTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVISEIRSALAQTINC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA