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The map label for this gene is hofB

Identifier: 218698527

GI number: 218698527

Start: 125818

End: 127203

Strand: Reverse

Name: hofB

Synonym: ECIAI39_0107

Alternate gene names: 218698527

Gene position: 127203-125818 (Counterclockwise)

Preceding gene: 218698528

Following gene: 218698526

Centisome position: 2.48

GC content: 53.46

Gene sequence:

>1386_bases
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAATCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTAGAACGTTACAGTCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTATCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTAGCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGTTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Upstream 100 bases:

>100_bases
CACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGCAAGCCTGCGAAGATGTCTTCCGCTTTGATG
ACGCCAACTAAGGAGCGGCA

Downstream 100 bases:

>100_bases
GCAACTCTGGCGCTGGCATGGCATAACCGGCGACGGCAATGCGCAAGATGGGATGCTATGGGCTGAGAGCCGTGCTTTGC
TGCTCATAGCACTACAGCAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHANRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTIEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPFIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Sequences:

>Translated_461_residues
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHANRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTIEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPFIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE
>Mature_461_residues
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHANRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTIEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPFIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Specific function: Unknown

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=461, Percent_Identity=97.6138828633406, Blast_Score=916, Evalue=0.0,
Organism=Escherichia coli, GI1789723, Length=390, Percent_Identity=43.5897435897436, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI87082188, Length=272, Percent_Identity=36.7647058823529, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 50711; Mature: 50711

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQME
CCCHHHHHHHHHHCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECHHHHH
GHANRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLH
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEEEECCEEC
PLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTIELAGNAVSFRIATLACRGGEKV
CCCCCCCCCCEEEEEEEHHHCCCCHHHHCCCCCCCEEEEECCCEEEEEEEEEEECCCHHH
VLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHCC
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAE
CCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEE
IAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRR
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFEVLPITPFIRQLISANTDVES
CCCCEEECCCCCCCCCCCCCCCCCEEEHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHH
LETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQME
CCCHHHHHHHHHHCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECHHHHH
GHANRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLH
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEEEECCEEC
PLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTIELAGNAVSFRIATLACRGGEKV
CCCCCCCCCCEEEEEEEHHHCCCCHHHHCCCCCCCEEEEECCCEEEEEEEEEEECCCHHH
VLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHCC
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAE
CCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEE
IAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRR
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFEVLPITPFIRQLISANTDVES
CCCCEEECCCCCCCCCCCCCCCCCEEEHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHH
LETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959070; 8202364; 9278503 [H]