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The map label for this gene is ccdA

Identifier: 218692934

GI number: 218692934

Start: 118046

End: 118324

Strand: Reverse

Name: ccdA

Synonym: p1ECUMN_0156

Alternate gene names: 218692934

Gene position: 118324-118046 (Counterclockwise)

Preceding gene: 218692936

Following gene: 218692933

Centisome position: 96.75

GC content: 46.24

Gene sequence:

>279_bases
TTGCATCATAATAATATATACTTATATGCATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGT
GACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGA
ATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAA
ATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Upstream 100 bases:

>100_bases
TAAGCCAGATTTTGATTTTTATTGGTTAACATAACGCAAGGTAAAATAAATAAGGCTTACTAAAAGTAAGATAACAGTAT
GTGTATTTGCGCGCTGATTT

Downstream 100 bases:

>100_bases
AATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACA
CGCCCGGGCGACGGATGGTG

Product: plasmid maintenance protein CcdA

Products: protein histidine; sugar phosphate [C]

Alternate protein names: Protein LetA [H]

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MHHNNIYLYAYPKYVKKRCAMKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIE
MNGSFADENRDW

Sequences:

>Translated_92_residues
MHHNNIYLYAYPKYVKKRCAMKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIE
MNGSFADENRDW
>Mature_92_residues
MHHNNIYLYAYPKYVKKRCAMKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIE
MNGSFADENRDW

Specific function: Binds to and blocks the activity of CcdB. Removes the bound LetD protein from the LetD-GyrA complex by forming a LetA- LetD complex [H]

COG id: COG5302

COG function: function code R; Post-segregation antitoxin (ccd killing mechanism protein) encoded by the F plasmid

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009956 [H]

Pfam domain/function: PF07362 CcdA [H]

EC number: NA

Molecular weight: Translated: 10867; Mature: 10867

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHNNIYLYAYPKYVKKRCAMKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLR
CCCCCEEEEECHHHHHHHHHHHCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
AERWKAENQEGMAEVARFIEMNGSFADENRDW
HHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHHNNIYLYAYPKYVKKRCAMKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLR
CCCCCEEEEECHHHHHHHHHHHCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
AERWKAENQEGMAEVARFIEMNGSFADENRDW
HHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-phosphohistidine; sugar [C]

Specific reaction: Protein N-phosphohistidine + sugar = protein histidine + sugar phosphate [C]

General reaction: Removes the bound letD protein from the letD-gyrA complex by forming a letA-letD complex [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA