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The map label for this gene is pemI

Identifier: 218692852

GI number: 218692852

Start: 49082

End: 49339

Strand: Reverse

Name: pemI

Synonym: p1ECUMN_0066

Alternate gene names: 218692852

Gene position: 49339-49082 (Counterclockwise)

Preceding gene: 218692853

Following gene: 218692851

Centisome position: 40.34

GC content: 53.49

Gene sequence:

>258_bases
ATGCATACCACCCGACTGAAGAGGGTTGGCGGCTCAGTTATGCTGACCGTCCCACCGGCACTGCTGAATGCGCTGTCTCT
GGGCACAGATAATGAAGTTGGCATGGTCATTGATAATGGCCGGCTGATTGTTGAGCCGTACAGACGCCCGCAATATTCAC
TGGCTGAGCTACTGGCACAGTGTGATCCGAATGCTGAAATATCAGCTGAAGAACGAGAATGGCTGGATGCACCGGCGACT
GGTCAGGAGGAAATCTGA

Upstream 100 bases:

>100_bases
TTGGTTGAAAGTTATATTTTTATTAAACATTGTGCGTTAAAGCCTGGTGTGTTTTTTTAGTGGATGTTATATTTAAATAT
AACTTTTATGGAGGTGAAGA

Downstream 100 bases:

>100_bases
CATGGAAAGAGGGGAAATCTGGCTTGTCTCGCTTGATCCTACCGCAGGTCATGAGCAGCAGGGAACGCGGCCGGTGCTGA
TTGTCACACCGGCGGCCTTT

Product: Plasmid stable inheritance repressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 85; Mature: 85

Protein sequence:

>85_residues
MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPAT
GQEEI

Sequences:

>Translated_85_residues
MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPAT
GQEEI
>Mature_85_residues
MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPAT
GQEEI

Specific function: Responsible for the stable maintenance of the plasmid during cell division. Both PemI and PemK proteins bind to the promoter region of the pem operon to autoregulate their synthesis

COG id: COG2336

COG function: function code T; Growth regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemI family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEMI_ECOLX (P13975)

Other databases:

- EMBL:   M26840
- EMBL:   X06240
- PIR:   I64783
- RefSeq:   NP_862962.1
- RefSeq:   NP_957646.1
- RefSeq:   YP_001096514.1
- RefSeq:   YP_002527568.1
- RefSeq:   YP_003108264.1
- RefSeq:   YP_003108328.1
- RefSeq:   YP_003829175.1
- RefSeq:   YP_003829285.1
- RefSeq:   YP_003937672.1
- ProteinModelPortal:   P13975
- SMR:   P13975
- GeneID:   1446523
- GeneID:   2716470
- GeneID:   4924839
- GeneID:   7324592
- GeneID:   8319206
- GeneID:   8319256
- GeneID:   9538106
- GeneID:   9538222
- GeneID:   9846000
- ProtClustDB:   CLSK861837
- InterPro:   IPR007159

Pfam domain/function: PF04014 SpoVT_AbrB

EC number: NA

Molecular weight: Translated: 9333; Mature: 9333

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAELLAQ
CCCCCCHHCCCEEEEEECHHHHHHHCCCCCCCCEEEEECCEEEECCCCCCCHHHHHHHHH
CDPNAEISAEEREWLDAPATGQEEI
CCCCCCCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAELLAQ
CCCCCCHHCCCEEEEEECHHHHHHHCCCCCCCCEEEEECCEEEECCCCCCCHHHHHHHHH
CDPNAEISAEEREWLDAPATGQEEI
CCCCCCCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019092; 3323833; 2832364; 8455570