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The map label for this gene is pemK

Identifier: 218692851

GI number: 218692851

Start: 48748

End: 49212

Strand: Reverse

Name: pemK

Synonym: p1ECUMN_0065

Alternate gene names: 218692851

Gene position: 49212-48748 (Counterclockwise)

Preceding gene: 218692852

Following gene: 218692850

Centisome position: 40.24

GC content: 55.05

Gene sequence:

>465_bases
TTGTTGAGCCGTACAGACGCCCGCAATATTCACTGGCTGAGCTACTGGCACAGTGTGATCCGAATGCTGAAATATCAGCT
GAAGAACGAGAATGGCTGGATGCACCGGCGACTGGTCAGGAGGAAATCTGACATGGAAAGAGGGGAAATCTGGCTTGTCT
CGCTTGATCCTACCGCAGGTCATGAGCAGCAGGGAACGCGGCCGGTGCTGATTGTCACACCGGCGGCCTTTAATCGCGTG
ACCCGCCTGCCTGTTGTTGTGCCCGTAACCAGCGGAGGCAATTTTGCCCGCACTGCCGGCTTTGCGGTGTCGTTGGATGG
TGTTGGCATACGTACCACAGGTGTTGTACGTTGCGATCAACCCCGGACAATTGATATGAAAGCACGGGGCGGAAAACGAC
TCGAACGGGTTCCGGAGACTATCATGAACGAAGTTCTTGGCCGCCTGTCCACTATTCTGACTTGA

Upstream 100 bases:

>100_bases
GGCGGCTCAGTTATGCTGACCGTCCCACCGGCACTGCTGAATGCGCTGTCTCTGGGCACAGATAATGAAGTTGGCATGGT
CATTGATAATGGCCGGCTGA

Downstream 100 bases:

>100_bases
ACATGGGGTTTGAGGGGCAACTGGATGAAAACGTACGATTTAGGGCACTAAAACCGCTGTTGTCCCACCATTCTGGTGAT
TCCCAAACGTTATTTGGCTA

Product: Plasmid stable inheritance protein

Products: NA

Alternate protein names: Endoribonuclease PemK; Kid toxin protein [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MLSRTDARNIHWLSYWHSVIRMLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRV
TRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVLGRLSTILT

Sequences:

>Translated_154_residues
MLSRTDARNIHWLSYWHSVIRMLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRV
TRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVLGRLSTILT
>Mature_154_residues
MLSRTDARNIHWLSYWHSVIRMLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRV
TRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVLGRLSTILT

Specific function: Part of a toxin-antitoxin (TA) module. Probably functions as an mRNA interferase. Responsible for the stable maintenance of the plasmid during cell division by postsegregational killing of plasmid-less daughter cells. Both pemI and PemK proteins bind to t

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

Organism=Escherichia coli, GI1790672, Length=114, Percent_Identity=43.859649122807, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 17447; Mature: 17447

Theoretical pI: Translated: 11.70; Mature: 11.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRTDARNIHWLSYWHSVIRMLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAG
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCC
HEQQGTRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQ
CCCCCCCCEEEECCHHHHHHCCCEEEEEEECCCCEEECCCEEEEECCCCEEEEEEEEECC
PRTIDMKARGGKRLERVPETIMNEVLGRLSTILT
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSRTDARNIHWLSYWHSVIRMLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAG
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCC
HEQQGTRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQ
CCCCCCCCEEEECCHHHHHHCCCEEEEEEECCCCEEECCCEEEEECCCCEEEEEEEEECC
PRTIDMKARGGKRLERVPETIMNEVLGRLSTILT
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019092; 3323833; 2832364; 8455570 [H]