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The map label for this gene is traC [H]

Identifier: 218698091

GI number: 218698091

Start: 5007016

End: 5008350

Strand: Reverse

Name: traC [H]

Synonym: EC55989_4901

Alternate gene names: 218698091

Gene position: 5008350-5007016 (Counterclockwise)

Preceding gene: 218698092

Following gene: 218698090

Centisome position: 97.16

GC content: 54.31

Gene sequence:

>1335_bases
TTGAAGATAACCACCTGTTGGTTACTGCCCTTCACACCAGCTGATTACCTCAGCATCCTTTACTCCTCGTGCCGGGCGAC
TCCCGACCGGGAGCGCTGTACCCGGCTTCTTTATCATGAGGTTACGCTTATGAACAGAAGCAACGATCTTTACCAGAAAG
TCACAGATGAAATTATCGCCGCGCTGGAGAAAGGCGTGCTCCCCTGGGTTCGCCCGTGGCGGGAAGGGGAACCGGTGGTG
CCGATGAATGCCCTGTCCGGGCGGTTTTATCACGGCATCAATATTCCCCTGCTCTGGAACAGCGCAGAACGACAGGGATA
TGAAAACGATCGCTGGCTGACCTTCACTCAAATTCGCAACGCGGGCGGTAACATCCACAAAGGCGAAAGATCTACACTGG
CCGTGTTTTATCTGCCCCAGCAGCGCGAAGTTGTGGACAGCAACGGAAATACGGTTCTTGATGCTGACGGCAACCCTAAA
GTGATGTCTTACGCTGTAGTACGCGAGTTCCGGCTGTTCAACATTCAACAATGTGAAGGGCTGCCGGAGGCGTTTTCACA
GCCTGTCGTGATGGTCGATGATCCTATCGCCGCCACTGAGCAGGTTACCCGGCAAAGTGCGGTGATGATCACTCACCGAC
GCCAGAACCGGGCGTATTACTCGCCGGGGCGCGACTGCATCATCATGCCGCATCCTGAGCAGTTCACTTCGCGTGAGGAT
TATTACGGCACGCTGCTGCATGAACTGACGCACGCTACCGGACACGCTTCACGATTGAACCGGGACGGCATCACTGCCGG
AAAGCATACCTTCGGCGATCCGACGTACAGTTTTGAAGAGCTGGTCGCCGAAATGGGAGCGGCTTTCCTGTGTGCTCACG
TTGGCATCCAGTCGAAGCTACAGCATGACAGTTACATCGCATCGTGGCTGAAGGTGTTGCAGCAAGACAAAAAAGCGATT
TTCCGCGCCAGCGGTTTAGCGCGTAATGCCTGTGAGTATCTGCTTGAACGGGCGCAGCAGCCCGTTAGCGCTGAGCGCAT
GACCTTCATATCAACGGCGGAGCATCCTGCTCTGCCGTTTTTCTCCACTCCAATAAATTTGCGCAACCCAATAAACAGTT
TTGCCCCAAATCTGACGGCACCAAAAGGCAAAATCCAAAGGGTTAAGGGGAAGAGAGTGAAGGGGGAACGGGCTTACCCG
AAAAGGGGCTTCCCTTCGTCTTTCGAGTTGCAGGTGCGTTGGCTTTCCTCGCTCACCCCAGTTACTTACTTATGTAAGCT
CCAGGGGATTCACTGCGTCACCGCCTTCCTGCACCTCAAAATCCATTGGGAATAA

Upstream 100 bases:

>100_bases
AGCGCAGGCCCGGACCGTAAGAGTATCAATTTCGGTCTGTACTGTTTGCCCTCTGACGGCAGCAGCAATGTACCGCTGAT
GACGCCGCTGCGCTTAACGC

Downstream 100 bases:

>100_bases
AGGGCCATTATGCGGATATGGCTGACGCGCAAAAAGCAAAACACCACACCATCCACTGGCGAACCAGACGTCACGGAGGG
ATGGCTGCATCCTGAATCCG

Product: hypothetical protein

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKITTCWLLPFTPADYLSILYSSCRATPDRERCTRLLYHEVTLMNRSNDLYQKVTDEIIAALEKGVLPWVRPWREGEPVV
PMNALSGRFYHGINIPLLWNSAERQGYENDRWLTFTQIRNAGGNIHKGERSTLAVFYLPQQREVVDSNGNTVLDADGNPK
VMSYAVVREFRLFNIQQCEGLPEAFSQPVVMVDDPIAATEQVTRQSAVMITHRRQNRAYYSPGRDCIIMPHPEQFTSRED
YYGTLLHELTHATGHASRLNRDGITAGKHTFGDPTYSFEELVAEMGAAFLCAHVGIQSKLQHDSYIASWLKVLQQDKKAI
FRASGLARNACEYLLERAQQPVSAERMTFISTAEHPALPFFSTPINLRNPINSFAPNLTAPKGKIQRVKGKRVKGERAYP
KRGFPSSFELQVRWLSSLTPVTYLCKLQGIHCVTAFLHLKIHWE

Sequences:

>Translated_444_residues
MKITTCWLLPFTPADYLSILYSSCRATPDRERCTRLLYHEVTLMNRSNDLYQKVTDEIIAALEKGVLPWVRPWREGEPVV
PMNALSGRFYHGINIPLLWNSAERQGYENDRWLTFTQIRNAGGNIHKGERSTLAVFYLPQQREVVDSNGNTVLDADGNPK
VMSYAVVREFRLFNIQQCEGLPEAFSQPVVMVDDPIAATEQVTRQSAVMITHRRQNRAYYSPGRDCIIMPHPEQFTSRED
YYGTLLHELTHATGHASRLNRDGITAGKHTFGDPTYSFEELVAEMGAAFLCAHVGIQSKLQHDSYIASWLKVLQQDKKAI
FRASGLARNACEYLLERAQQPVSAERMTFISTAEHPALPFFSTPINLRNPINSFAPNLTAPKGKIQRVKGKRVKGERAYP
KRGFPSSFELQVRWLSSLTPVTYLCKLQGIHCVTAFLHLKIHWE
>Mature_444_residues
MKITTCWLLPFTPADYLSILYSSCRATPDRERCTRLLYHEVTLMNRSNDLYQKVTDEIIAALEKGVLPWVRPWREGEPVV
PMNALSGRFYHGINIPLLWNSAERQGYENDRWLTFTQIRNAGGNIHKGERSTLAVFYLPQQREVVDSNGNTVLDADGNPK
VMSYAVVREFRLFNIQQCEGLPEAFSQPVVMVDDPIAATEQVTRQSAVMITHRRQNRAYYSPGRDCIIMPHPEQFTSRED
YYGTLLHELTHATGHASRLNRDGITAGKHTFGDPTYSFEELVAEMGAAFLCAHVGIQSKLQHDSYIASWLKVLQQDKKAI
FRASGLARNACEYLLERAQQPVSAERMTFISTAEHPALPFFSTPINLRNPINSFAPNLTAPKGKIQRVKGKRVKGERAYP
KRGFPSSFELQVRWLSSLTPVTYLCKLQGIHCVTAFLHLKIHWE

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 50529; Mature: 50529

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITTCWLLPFTPADYLSILYSSCRATPDRERCTRLLYHEVTLMNRSNDLYQKVTDEIIA
CCEEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ALEKGVLPWVRPWREGEPVVPMNALSGRFYHGINIPLLWNSAERQGYENDRWLTFTQIRN
HHHHCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCEEEECCHHHCCCCCCCEEEEEHHHC
AGGNIHKGERSTLAVFYLPQQREVVDSNGNTVLDADGNPKVMSYAVVREFRLFNIQQCEG
CCCCCCCCCCCEEEEEECCCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHCC
LPEAFSQPVVMVDDPIAATEQVTRQSAVMITHRRQNRAYYSPGRDCIIMPHPEQFTSRED
CHHHHCCCEEEECCCCHHHHHHHHHHHEEEEEECCCCCEECCCCCEEEECCCHHHCCCCH
YYGTLLHELTHATGHASRLNRDGITAGKHTFGDPTYSFEELVAEMGAAFLCAHVGIQSKL
HHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHH
QHDSYIASWLKVLQQDKKAIFRASGLARNACEYLLERAQQPVSAERMTFISTAEHPALPF
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCC
FSTPINLRNPINSFAPNLTAPKGKIQRVKGKRVKGERAYPKRGFPSSFELQVRWLSSLTP
CCCCCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCH
VTYLCKLQGIHCVTAFLHLKIHWE
HHHHHHHCCHHHEEEEEEEEEEEC
>Mature Secondary Structure
MKITTCWLLPFTPADYLSILYSSCRATPDRERCTRLLYHEVTLMNRSNDLYQKVTDEIIA
CCEEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ALEKGVLPWVRPWREGEPVVPMNALSGRFYHGINIPLLWNSAERQGYENDRWLTFTQIRN
HHHHCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCEEEECCHHHCCCCCCCEEEEEHHHC
AGGNIHKGERSTLAVFYLPQQREVVDSNGNTVLDADGNPKVMSYAVVREFRLFNIQQCEG
CCCCCCCCCCCEEEEEECCCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHCC
LPEAFSQPVVMVDDPIAATEQVTRQSAVMITHRRQNRAYYSPGRDCIIMPHPEQFTSRED
CHHHHCCCEEEECCCCHHHHHHHHHHHEEEEEECCCCCEECCCCCEEEECCCHHHCCCCH
YYGTLLHELTHATGHASRLNRDGITAGKHTFGDPTYSFEELVAEMGAAFLCAHVGIQSKL
HHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHH
QHDSYIASWLKVLQQDKKAIFRASGLARNACEYLLERAQQPVSAERMTFISTAEHPALPF
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCC
FSTPINLRNPINSFAPNLTAPKGKIQRVKGKRVKGERAYPKRGFPSSFELQVRWLSSLTP
CCCCCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCH
VTYLCKLQGIHCVTAFLHLKIHWE
HHHHHHHCCHHHEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]