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The map label for this gene is gltS [H]

Identifier: 218698043

GI number: 218698043

Start: 4970396

End: 4971535

Strand: Reverse

Name: gltS [H]

Synonym: EC55989_4853

Alternate gene names: 218698043

Gene position: 4971535-4970396 (Counterclockwise)

Preceding gene: 218698047

Following gene: 218698042

Centisome position: 96.44

GC content: 38.95

Gene sequence:

>1140_bases
ATGATCCTAGATGCCAGTTATACATTATTAGTCGCATGTATCGCGCTACTCATAGGAATGTTTGTCGTAAAATTTACCCC
GTTCCTACAAAAAAACCACATACCAGAAGCCGTCGTTGGTGGCTTTATTGTTGCAATTGTTCTGTTAATTATTGATAAAA
CATCAGGTTATTCGTTTACTTTTGATGCTTCATTGCAAAGTTTATTAATGCTCACATTCTTTTCCTCTATCGGGCTAAGT
TCTGACTTTTCTCGACTGATTAAAGGAGGAAAGCCGTTAGTTCTATTAACTATTGCAGTAACGATCCTAATCGCCATTCA
AAATACTGTCGGCATGAGTATGGCTGTCATGATGAATGAAAGTCCATTTATTGGCTTAATTGCAGGTTCAATTACTCTAA
CAGGTGGTCATGGTAATGCCGGAGCATGGGGCCCTATTCTCGCTGATAAATATGGTGTAACAGGCGCCGTTGAATTAGCG
ATGGCTTGTGCAACACTTGGATTAGTGTTGGGTGGTTTAGTTGGCGGCCCTGTTGCCCGTCATCTTTTGAAAAAGGTCTC
TATTCCTAAAACAACCGAGCAAGAGCGCGACACTATCGTTGAAGCTTTTGAGCAACCAAGCGTCAAAAGAAAAATCAATG
CAAATAACGTTATTGAAACCATTTCAATGCTGATTATCTGTATTGTTGTTGGTGGCTATATCAGTGCATTGTTTAAAGAT
ACTTTTCTGCAACTGCCTACTTTTGTCTGGTGTTTATTTGTCGGTATTATTATCCGTAATACACTGACTCATGTATTTAA
ACACGAAGTGTTTGAGCCGACCGTCGATGTATTAGGTAGCGTTGCTTTATCGCTTTTCTTGGCAATGGCGTTAATGTCAT
TAAAATTTGGTCAATTGGCAAGCATGGCAGGGCCAGTATTAATTATCATTGCTGTACAAACTGTTGTCATGGTGCTATTT
GCCTGCTTTGTCCTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGA
TCAAATGGTTTCGCCAAAAATCAATAATCAGACAACAAAATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATT
CTGCCCCGAATTACACTTAA

Upstream 100 bases:

>100_bases
AAAACTCATTGTTAAAGATTAGTTAACCAAAGTAACTTCATTTTTCACTATTGTTAAATTGTTATAGTGAAAATTCTGGT
AAACTAATAGAGGTAAAAAA

Downstream 100 bases:

>100_bases
AACGACTCAACAGCTTAACGTTGGCTTGCCACGCCTTACTTGACTGTAAAACTCTCACTCTTACCGAACTTGGCCGTAAC
CTGCCAACCAAAGCGAGAAC

Product: conserved hypothetical protein JemA

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MILDASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFTFDASLQSLLMLTFFSSIGLS
SDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMMNESPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELA
MACATLGLVLGGLVGGPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYISALFKD
TFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALMSLKFGQLASMAGPVLIIIAVQTVVMVLF
ACFVLMNPLMILVKIIKLRWIHILSYDQMVSPKINNQTTKCANSIFYTTLFTNSAPNYT

Sequences:

>Translated_379_residues
MILDASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFTFDASLQSLLMLTFFSSIGLS
SDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMMNESPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELA
MACATLGLVLGGLVGGPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYISALFKD
TFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALMSLKFGQLASMAGPVLIIIAVQTVVMVLF
ACFVLMNPLMILVKIIKLRWIHILSYDQMVSPKINNQTTKCANSIFYTTLFTNSAPNYT
>Mature_379_residues
MILDASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFTFDASLQSLLMLTFFSSIGLS
SDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMMNESPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELA
MACATLGLVLGGLVGGPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYISALFKD
TFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALMSLKFGQLASMAGPVLIIIAVQTVVMVLF
ACFVLMNPLMILVKIIKLRWIHILSYDQMVSPKINNQTTKCANSIFYTTLFTNSAPNYT

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=306, Percent_Identity=47.7124183006536, Blast_Score=234, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 40932; Mature: 40932

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFT
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
FDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMMNE
EHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECC
SPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELAMACATLGLVLGGLVGGPVAR
CCCEEEEEEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
HLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYISALFKD
HHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALMSLKFGQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMAGPVLIIIAVQTVVMVLFACFVLMNPLMILVKIIKLRWIHILSYDQMVSPKINNQTTK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
CANSIFYTTLFTNSAPNYT
HHHHHHHHHEEECCCCCCC
>Mature Secondary Structure
MILDASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFT
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
FDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMMNE
EHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECC
SPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELAMACATLGLVLGGLVGGPVAR
CCCEEEEEEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
HLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYISALFKD
HHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALMSLKFGQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMAGPVLIIIAVQTVVMVLFACFVLMNPLMILVKIIKLRWIHILSYDQMVSPKINNQTTK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
CANSIFYTTLFTNSAPNYT
HHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]