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The map label for this gene is fumB

Identifier: 218697824

GI number: 218697824

Start: 4729930

End: 4731576

Strand: Reverse

Name: fumB

Synonym: EC55989_4613

Alternate gene names: 218697824

Gene position: 4731576-4729930 (Counterclockwise)

Preceding gene: 218697825

Following gene: 218697823

Centisome position: 91.79

GC content: 54.52

Gene sequence:

>1647_bases
ATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGA
TTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAG
CCTTTCACGACGCTTCTTTTATGCTCCGCCCGGCACACCAGAAACAGGTTGCGGCTATTCTTCACGATCCAGAAGCCAGC
GAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCCGCCAAAGGCGTGCTGCCGACCTGCCAGGA
TACCGGCACCGCGATCATCGTCGGTAAAAAAGGCCAGCGCGTGTGGACCGGTGGCGGTGATGAAGAAGCGCTGTCGAAAG
GCGTCTATAACACCTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACC
GGCACTAACCTGCCTGCGCAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAAATTCCTTTGCGTTGCGAAAGGCGG
CGGCTCTGCCAACAAAACGTATCTCTACCAGGAAACCAAAGCCCTGCTGACTCCCGGCAAACTGAAAAACTTCCTCGTCG
AGAAAATGCGTACCCTCGGTACTGCAGCCTGCCCGCCGTACCATATCGCGTTTGTGATTGGCGGTACGTCTGCGGAAACC
AACCTGAAAACCGTCAAGTTAGCAAGCGCTCACTATTACGATGAACTGCCGACGGAAGGGAACGAACATGGTCAGGCGTT
CCGCGATGTCCAGCTGGAACAGGAACTGCTGGAAGAGGCCCAGAAACTCGGTCTTGGCGCGCAGTTTGGCGGTAAATACT
TCGCGCACGACATTCGCGTTATCCGTCTGCCACGTCACGGCGCATCCTGCCCGGTCGGCATGGGCGTCTCCTGCTCCGCT
GACCGTAACATTAAAGCGAAAATCAACCGCGAAGGTATCTGGATCGAAAAACTGGAACACAACCCAGGCCAGTACATTCC
ACAAGAACTGCGCCAGGCCGGTGAAGGCGAAGCGGTGAAAGTTGACCTTAACCGCCCGATGAAAGAGATCCTCGCCCAGC
TTTCGCAATACCCGGTATCCACTCGTTTGTCGCTCACCGGCACCATTATCGTGGGCCGAGATATTGCACACGCCAAGCTG
AAAGAGCTGATTGACGCCGGTAAAGAACTTCCGCAGTACATCAAAGATCACCCGATCTACTACGCGGGTCCGGCGAAAAC
CCCTGCCGGTTATCCATCAGGTTCACTTGGCCCAACCACCGCAGGTCGTATGGACTCCTACGTGGATCTGCTGCAATCCC
ACGGCGGCAGCATGATCATGCTAGCGAAAGGTAACCGCAGTCAGCAGGTTACCGACGCGTGTCATAAACACGGCGGCTTC
TACCTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATCTGGAATGCGTCGCTTATCCGGA
GCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACT
TCTTCCAGCAGATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAA

Upstream 100 bases:

>100_bases
CCGCGATGTACGGGTTCTTATAAATGCACTTTGCGTGCCGCCCGTAACTACGCGGCACGCCATTTTCGAATAACAAATAC
AGAGTTACAGGCTGGAAGCT

Downstream 100 bases:

>100_bases
CCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGTGATCCCCCTGGGCCACCTCTTTTGCGATTGTAATTTCACGCTT
GCTGGTGAATAGTCAGTATT

Product: anaerobic class I fumarate hydratase (fumarase B)

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEAS
ENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET
NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKL
KELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK

Sequences:

>Translated_548_residues
MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEAS
ENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET
NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKL
KELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK
>Mature_547_residues
SNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASE
NDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNTG
TNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAETN
LKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINREGIWIEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLK
ELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGFY
LGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=99.8175182481752, Blast_Score=1137, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=90.1459854014599, Blast_Score=1030, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=29.8342541436464, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60076; Mature: 59945

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASF
CCCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCEEEEECHHHHHHHHHHHHCCCHH
MLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHEEECCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EECCCCCHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRV
CEEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIPQELRQAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKELIDAGKELPQYIKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEE
YAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVN
EEECCCCHHHHHHHHHHHHHEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KQCANCTK
HHHCCCCC
>Mature Secondary Structure 
SNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASF
CCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCEEEEECHHHHHHHHHHHHCCCHH
MLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHEEECCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EECCCCCHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQFGGKYFAHDIRV
CEEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIPQELRQAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKELIDAGKELPQYIKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEE
YAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVN
EEECCCCHHHHHHHHHHHHHEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KQCANCTK
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]