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The map label for this gene is phnE

Identifier: 218697806

GI number: 218697806

Start: 4707197

End: 4708039

Strand: Reverse

Name: phnE

Synonym: EC55989_4594

Alternate gene names: 218697806

Gene position: 4708039-4707197 (Counterclockwise)

Preceding gene: 218697807

Following gene: 218697805

Centisome position: 91.33

GC content: 62.04

Gene sequence:

>843_bases
GTGCAGGCGTTGATGCCGGATGCGGTGCAAGCACCTTATCCGGCCTACAGACCGGAGCCAAACATGCAAACCATCACCAT
CGCCCCACCCAAGCGTAGCTGGTTCTCGCTTCTGAGCTGGGCCGTTGTTCTCGCCGTGTTGGTCGTCTCGTGGCAGGGCG
CGGAAATGGCCCCGCTCACGCTGATTAAAGACGGCGGCAACATGGCGACGTTCGCCGCCGACTTCTTCCCGCCCGATTTC
AGCCAGTGGCAGGATTACCTCACCGAAATGGCCGTCACGCTGCAAATCGCCGTCTGGGGCACGGCGCTGGCGGTGGTTCT
CTCCATCCCCTTCGGCCTGATGAGCGCCGAAAACCTGGTGCCGTGGTGGGTTTACCAGCCCGTTCGCCGCCTGATGGACG
CCTGCCGCGCCATTAACGAAATGGTCTTCGCCATGCTGTTCGTGGTCGCCGTCGGCCTCGGCCCGTTCGCTGGCGTGCTG
GCGCTGTTTATCCACACCACCGGCGTGCTCTCCAAACTGCTTTCCGAAGCGGTGGAAGCGATTGAACCCGGCCCGGTGGA
AGGCATTCGCGCCACCGGCGCCAACAAGCTCGAAGAGATCCTCTACGGCGTGCTGCCGCAGGTGATGCCGCTGCTGATCT
CCTACTCCCTCTATCGCTTCGAATCCAACGTCCGCTCGGCGACCGTCGTCGGCATGGTCGGTGCAGGCGGGATCGGCGTC
ACCCTGTGGGAAGCGATTCGCGGTTTCCAGTTCCAACAAACCTGCGCCCTGATGGTGCTTATCATCGTCACGGTCAGCCT
GCTGGATTTCCTCTCTCAACGGTTGCGTAAGCACTTTATCTGA

Upstream 100 bases:

>100_bases
GGACCGCCTGAACAACGCGTTAAGCGCGATGAGTTCGGTGAGTAAAGCGGTGCAGTAAATCGTAGGTCGGATAAGACGCC
CCGGCGTCGCATCCGACAAT

Downstream 100 bases:

>100_bases
TAAGCGAGGCATTGATATCTATGCACTTGTCTACACATCCGACCAGCTACCCAACACGCTATCAGGAGATAGCCGCAAAA
CTTGAGCAGGAGCTTCGTCA

Product: phosphonates transport system, permease protein

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MQALMPDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDF
SQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVL
ALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGV
TLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI

Sequences:

>Translated_280_residues
MQALMPDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDF
SQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVL
ALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGV
TLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI
>Mature_280_residues
MQALMPDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDF
SQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVL
ALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGV
TLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30726; Mature: 30726

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALMPDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLT
CCCCCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LIKDGGNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLV
EEECCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IEPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGV
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCHHH
TLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQALMPDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLT
CCCCCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LIKDGGNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLV
EEECCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IEPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGV
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCHHH
TLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]