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The map label for this gene is ptsA

Identifier: 218697662

GI number: 218697662

Start: 4526864

End: 4529365

Strand: Reverse

Name: ptsA

Synonym: EC55989_4430

Alternate gene names: 218697662

Gene position: 4529365-4526864 (Counterclockwise)

Preceding gene: 218697668

Following gene: 218697661

Centisome position: 87.87

GC content: 55.08

Gene sequence:

>2502_bases
ATGGCCCTGATTGTGGAATTTATTTGTGAGCTACCTAACGGCGTACATGCACGTCCGGCAAGCCACGTTGAAACGCTGTG
TAATACTTTTTCATCACAAATTGAGTGGCATAACCTGCGCACTGACCGCAAGGGCAACGCCAAAAGCGCCCTTGCGCTGA
TTGGCACCGATACGCTGGCGGGCGATAACTGCCAGTTACTGATTTCCGGGGCCGACGAACAGGAAGCGCACCAGCGTTTA
AGCCAATGGCTGCGCGATGAATTCCCCCACTGCGATGCGCCGCTGGCGGAAGTTAAATCTGACGAACTGGAACCACTGCC
GGTTTCACTGACCAATCTGAATCCGCAAATTATCCGCGCCCGCACCGTGTGCAGCGGTAGTGCAGGCGGCATTCTGACGC
CGATCTCTTCTTTAGATCTCAATGCGCTGGGTAATCTTCCCGCAGCCAAAAGCGTTGACGCCGAGCAATCCGCACTGGAA
AACGGCCTGACGCTGGTACTGAAAAACATTGAGTTTCGTCTGCTGGATAGCGACGGTGCCACCAGCGCGATTCTGGAAGC
GCACCGATCCCTGGCTGGCGATACTTCCCTGCGCGAACATTTACTGGCAGGCGTCAGCGCCGGATTAAGCTGCGCCGAAG
CGATTGTCACCAGTGCGAATCACTTTTGCGAAGAGTTCGCCCGTTCCAGCAGCAGCTACCTGCAAGAACGTGCCCTGGAC
GTACGCGACGTCTGCTTCCAGTTACTCCAGCAAATCTACGGTGAGCAACGCTTCCCGGCACCGGGCAAACTGACGCAGCC
CGCCATTTGTATGGCTGATGAACTGACTCCCAGCCAGTTCCTCGAACTGGATAAAAATCACCTCAAAGGTCTGTTACTGA
AAAGTGGCGGCACCACCTCACATACCGTAATTCTTGCCCGTTCGTTCAACATTCCGACACTGGTTGGTGTAGATATTGAT
GCCCTTACTCCGTGGCAGCATCAAACGATTTATATCGACGGCAACGCTGGGGCGATTGTGGTTGAGCCAGGAGAAGCCGT
AGCTCGTTATTATCAGCAAGAAGCCCGCGTACAGGACGCCCTGCGTGAGCAACAGCGTGTCTGGCTGACCCAACAAGCCC
GTACCGCTGACGGTATCCGCATTGAAATTGCCGCTAACATCGCTCACTCCGTGGAAGCGCAGGCCGCATTCGGCAATGGT
GCGGAAGGCGTTGGTTTGTTCCGCACTGAAATGCTCTATATGGATCGCACCAGTGCACCGGGCGAAAGCGAGCTGTACAA
CATTTTTTGTCAGGCGCTGGAGTCTGCCAACGGACGCAGCATTATTGTGCGCACTATGGACATTGGCGGCGACAAACCCG
TTGATTATCTGAACATTCCGGCAGAAGCTAACCCGTTCCTCGGTTATCGCGCTGTGCGTATTTATGAAGAATACGCCTCG
CTGTTCACCACACAACTACGGTCGATCCTGCGCGCCTCCGCTCACGGCAGCCTGAAAATCATGATCCCGATGATCTCCTC
AATGGAAGAGATCTTATGGGTGAAAGAAAAACTGGCGGAAGCCAAACAGCAACTGCGTAACGAACACATTCCGTTTGATG
AGAAGATCCAGCTTGGCATTATGCTGGAAGTACCGTCGGTGATGTTCATCATCGATCAATGCTGCGAAGAGACTGATTTC
TTTAGTATTGGTAGTAATGACCTGACGCAATATCTGCTGGCAGTGGATCGCGATAACGCTAAGGTTACTCGTCACTACAA
CAGCCTGAATCCGGCATTCTTGCGGGCGCTCGATTACGCCGTGCAGGCGGTGCATCGCCAGGGCAAATGGATTGGTCTGT
GCGGTGAGCTGGGAGCGAAAGGTTCCGTGCTGCCGTTGCTGGTCGGCTTAGGGCTGGATGAACTCAGCATGAGCGCACCA
TCAATTCCGGCGGCGAAAGCGCGGATGGCGCAACTTGATAGCCGTGAGTGCCGCAAGTTGCTCAACCAAGCAATGGCCTG
CCGTACTTCGCTGGAAGTGGAACACCTGCTGGCGCAATTCCGCATGACCCAACAGGACGCACCGCTGGTCACCGCCGAGT
GCATCACGCTGGAAAGTGACTGGCGCAGCAAAGAAGAAGTGCTCAAAGGCATGACCGATAACCTGCTGCTGGCGGGCCGC
TGCCGCTATCCGCGTAAACTGGAAGCCGACTTATGGGCGCGCGAGGCCGTTTTCTCTACCGGTCTGGGCTTTAGTTTTGC
CATTCCACACAGCAAATCAGAACACATTGAGCAATCCACCATCAGCGTGGCGCGTCTGCAAGCGCCGGTGCGCTGGGGCG
ATGATGAAGCGCAATTCATCATTATGTTAACCCTGAACAAACACGCTGCGGGCGATCAGCACATGCGCATTTTCTCGCGC
CTCGCTCGCCGCATCATGCACGAAGAATTCCGTAACGCGCTGGTTAACGCCGCCTCTGCCGACGCTATCGCCAGCCTGCT
GCAACATGAACTGGAACTGTAA

Upstream 100 bases:

>100_bases
TTACATTACATTTGTCCAGCTTTTGGCAGATTTGTCACATGGCAGGCCGTTGCCGTGCGCTCTTATCCTGTAAACAGAAA
ACCGTACCGGGAGAGAGGCA

Downstream 100 bases:

>100_bases
AAGGAAACATCATGGAACTGTATCTGGACACCGCTAACGTCGCAGAAGTCGAACGTCTGGCACGCATATTCCCCATTGCC
GGGGTGACAACTAACCCGAG

Product: fused putative PTS enzymes: Hpr component ; enzyme I component ; enzyme IIA component

Products: NA

Alternate protein names: MTP 2; Phosphoenolpyruvate-protein phosphotransferase; Enzyme I-Ani; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-like phosphotransferase enzyme IIA component; PTS system fructose-like EIIA component [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRL
SQWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALE
NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALD
VRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDID
ALTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNG
AEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYAS
LFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEETDF
FSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAP
SIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGR
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSR
LARRIMHEEFRNALVNAASADAIASLLQHELEL

Sequences:

>Translated_833_residues
MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRL
SQWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALE
NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALD
VRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDID
ALTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNG
AEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYAS
LFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEETDF
FSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAP
SIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGR
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSR
LARRIMHEEFRNALVNAASADAIASLLQHELEL
>Mature_832_residues
ALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLS
QWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALEN
GLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALDV
RDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDA
LTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNGA
EGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYASL
FTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEETDFF
SIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPS
IPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRC
RYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRL
ARRIMHEEFRNALVNAASADAIASLLQHELEL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI48994992, Length=833, Percent_Identity=99.3997599039616, Blast_Score=1705, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=834, Percent_Identity=44.9640287769784, Blast_Score=693, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=458, Percent_Identity=39.5196506550218, Blast_Score=331, Evalue=1e-91,
Organism=Escherichia coli, GI1789193, Length=502, Percent_Identity=33.8645418326693, Blast_Score=288, Evalue=7e-79,
Organism=Escherichia coli, GI1787994, Length=227, Percent_Identity=29.5154185022026, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1786951, Length=153, Percent_Identity=24.8366013071895, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 91816; Mature: 91685

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00369 PTS_HPR_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLA
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEECCCCC
GDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRA
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEEECCCHHHEEE
RTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALENGLTLVLKNIEFRLLDSDGA
EEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHCCCEEEEECCEEEEECCCCC
TSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
VRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCHHHHCEEEEECCCCCC
HTVILARSFNIPTLVGVDIDALTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDA
EEEEEEEECCCCEEEECCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHH
LREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEMLYMDRTSAP
HHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHEEEEECCCCC
GESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYAS
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
LFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGI
HHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
MLEVPSVMFIIDQCCEETDFFSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYA
EEECHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHHHHH
VQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKL
HHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHH
LNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCCEEEECC
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFI
CCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
IMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAASADAIASLLQHELEL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEECCCCC
GDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRA
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEEECCCHHHEEE
RTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALENGLTLVLKNIEFRLLDSDGA
EEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHCCCEEEEECCEEEEECCCCC
TSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
VRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCHHHHCEEEEECCCCCC
HTVILARSFNIPTLVGVDIDALTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDA
EEEEEEEECCCCEEEECCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHH
LREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEMLYMDRTSAP
HHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHEEEEECCCCC
GESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYAS
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
LFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGI
HHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
MLEVPSVMFIIDQCCEETDFFSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYA
EEECHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHHHHH
VQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKL
HHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHH
LNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCCEEEECC
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFI
CCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
IMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAASADAIASLLQHELEL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]