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The map label for this gene is yihU [H]

Identifier: 218697591

GI number: 218697591

Start: 4448978

End: 4450057

Strand: Direct

Name: yihU [H]

Synonym: EC55989_4353

Alternate gene names: 218697591

Gene position: 4448978-4450057 (Clockwise)

Preceding gene: 218697588

Following gene: 218697592

Centisome position: 86.31

GC content: 48.61

Gene sequence:

>1080_bases
GTGATACCCACGCATGCAATTTTGGTCATATTTGTCTTGTCCTGTTTAATTTTGTCGTCTTCGGTCAATTTAAATGACGC
AAATGATCAAATTAGTGATGCGTGTCGTGTTTTTGAACATAAATTACAAATATGCTCAAAAACAGACGAAAACGAATTTT
CAATTGTCATTATTTTGTCAATGTCAGACAGGAGAGTATTCATGGCAACCATTGCATTTCTCGGGCTGGGGCAGATGGGT
AGCCCAATGGCTAACAATCTATTGCAAAAAGGGCATTCATTGCAGGTATTTGATGTCAATGCACAGGCCGTCGATGCGCT
AGTTACGCAGGGGGCCACTGCGGCACAAACCCCAGCGGAAGCGGCAGCAGGCGCTGAATTTATCATCACTATGCTTCCCA
ATGGTGACATTGTTCGACAGGTTCTGTTGGGGGAAAAAGGCGTCTGTGAGGCTGTTTCATCTGACGCGTTATTGATTGAT
ATGTCGACTATTCATCCCTTACAGACCGATGCATTAATACGGGAGTTACAAGAAAAAGGCATCAGTATGATGGACGCCCC
CGTGGGCAGAACGTCCGTGAATGCGATTGATGGCACATTGCTGATTTTAGCGGGTGGCACTGATGAGCAGATTGCACGTG
CACGCCCGATTCTCATGTGCATGGGTAATGAGCTGGTCGAGGCTGGCGGCCCAGGAATGGGGATCCGCGTGAAGCTAATT
AACAACTATATGAGCATTGCGCTTAATGCGCTGTCCTCCGAGGCCGCTGTGTTATGTGAGTCACTGGGATTAAATCTTGA
TGTCGCAATTAAGGTGATGAGTGGTACGGCAGCAGGGAAAGGACATTTTACGACCACCTGGCCTTGCAAAGTGCTGAAAG
GCGATCTTTCACCCGCCTTCATGGTGGATCTGGCGTTGAAAGATCTGCGTATTGCAGTTGACGTCGCTCGGGAGACGGGG
GCACCGCTCAATATGGGGATTGCAGCAGAAAGTTATTACGCTGCAGCAAGTCAAGACGGCAAAGGGCGGCAGGACTGGAG
TGCCCTGCTAAATCAGGTTCGCCAGCAGGCTGGTCTTTAA

Upstream 100 bases:

>100_bases
TGTTGCTGCCGGGCCGCCACCAATCTCAAGATAATGATTTGCCTGATATTTTCCGCCGCCATCCGGCAGAGTGAGCAAAG
AATAAATCCTGTCCTGAACT

Downstream 100 bases:

>100_bases
TTTTATCTATTAACAGGAGTTTCTATGTCATCCCACTTCACCCCATTTACGCTGAAAGATATCTCCCGCCCAGGTGGTGG
ATTTGCCATGTTAGCTGTCG

Product: putative oxidoreductase with NAD(P)-binding Rossmann-fold domain, putative 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MIPTHAILVIFVLSCLILSSSVNLNDANDQISDACRVFEHKLQICSKTDENEFSIVIILSMSDRRVFMATIAFLGLGQMG
SPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLID
MSTIHPLQTDALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVEAGGPGMGIRVKLI
NNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETG
APLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL

Sequences:

>Translated_359_residues
MIPTHAILVIFVLSCLILSSSVNLNDANDQISDACRVFEHKLQICSKTDENEFSIVIILSMSDRRVFMATIAFLGLGQMG
SPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLID
MSTIHPLQTDALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVEAGGPGMGIRVKLI
NNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETG
APLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL
>Mature_359_residues
MIPTHAILVIFVLSCLILSSSVNLNDANDQISDACRVFEHKLQICSKTDENEFSIVIILSMSDRRVFMATIAFLGLGQMG
SPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLID
MSTIHPLQTDALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVEAGGPGMGIRVKLI
NNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETG
APLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=294, Percent_Identity=32.312925170068, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=26.056338028169, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=72.6027397260274, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=38.2142857142857, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1786719, Length=282, Percent_Identity=30.4964539007092, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789092, Length=283, Percent_Identity=29.3286219081272, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=313, Percent_Identity=28.7539936102236, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24655230, Length=323, Percent_Identity=31.2693498452012, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19922568, Length=323, Percent_Identity=31.2693498452012, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24655240, Length=93, Percent_Identity=40.8602150537634, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574115, Length=299, Percent_Identity=21.7391304347826, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 37970; Mature: 37970

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPTHAILVIFVLSCLILSSSVNLNDANDQISDACRVFEHKLQICSKTDENEFSIVIILS
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
MSDRRVFMATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAE
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCHH
AAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQTDALIRELQEKG
HHCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCC
ISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVEAGGPGMGIRVKLI
CEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCEEEECCCHHHHCCCCCCEEEEEEH
NNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAF
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCEEECCHHHEECCCCHHH
MVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL
HHHHHHHHHHHHEEEHHHCCCCCEECEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPTHAILVIFVLSCLILSSSVNLNDANDQISDACRVFEHKLQICSKTDENEFSIVIILS
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
MSDRRVFMATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAE
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCHH
AAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQTDALIRELQEKG
HHCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCC
ISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVEAGGPGMGIRVKLI
CEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCEEEECCCHHHHCCCCCCEEEEEEH
NNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAF
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCEEECCHHHEECCCCHHH
MVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL
HHHHHHHHHHHHEEEHHHCCCCCEECEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503 [H]