| Definition | Escherichia coli 55989, complete genome. |
|---|---|
| Accession | NC_011748 |
| Length | 5,154,862 |
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The map label for this gene is metE
Identifier: 218697550
GI number: 218697550
Start: 4397110
End: 4399371
Strand: Direct
Name: metE
Synonym: EC55989_4306
Alternate gene names: 218697550
Gene position: 4397110-4399371 (Clockwise)
Preceding gene: 218697548
Following gene: 218697552
Centisome position: 85.3
GC content: 55.84
Gene sequence:
>2262_bases ATGACAATTCTTAATCACACCCTCGGTTTCCCTCGCGTTGGCCTGCGTCGCGAGCTGAAAAAAGCGCAAGAAAGTTATTG GGCGGGGAACTCCACGCGTGAAGAACTGCTGACAGTAGGGCGTGAACTGCGTGCTCGTCACTGGGATCAACAAAAGCAAG CGGGTATCGACCTGCTGCCGGTGGGCGATTTTGCCTGGTACGATCATGTTCTGACCACCAGTCTGCTGCTGGGTAACGTT CCGCCACGTCATCAGAACAAAGACGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGG CGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCC AACAGTTCAAACTGACCTGGACGCAGCTGCTGGAGGAAGTGGACGAGGCGCTGGCGCTGGGCCACAACGTTAAGCCTGTG CTGCTGGGGCCGGTTACTTACCTGTGGCTGGGGAAAGTGAAAGGTGAACAGTTTGACCGCCTTAGCCTGCTGAACGACAT TCTGCCGGTTTATCAGCAAATGCTGGCAGAACTGGCGAAACGCGGCATCGAGTGGGTACAGATTGATGAACCCGCGCTGG TACTGGAACTACCACAGGCGTGGCTGGACGCATACAAACCCGCTTACGACGCGCTCCAGGGCCAGGTGAAACTGCTGCTG ACCACTTATTTTGAAGGCGTAACGCCAAACCTCGACACGATTACTGCGCTGCCTGTTCAGGGGCTGCATGTGGACCTTGT ACATGGTAAAGATGACGTTGTTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC GTAACGTCTGGCGTGCCGATCTTAGCGAGAAATATGCGCAAATTAAGGACATTGTCGGCAAACGTGATTTGTGGGTGGCA TCTTCCTGCTCGTTGCTGCACAGCCCCATCGACCTGAGCGTGGAAACGCGTCTTGATGCAGAAGTGAAAAGCTGGTTTGC CTTCGCCCTACAAAAATGCCATGAACTGGCACTGCTGCGCGATGCGCTGAACAGTGGTGACACGGCAGCTCTGGCAGAGT GGAGCGCCCCGATTCAGGCGCGTCGTCACTCTACTCGTGTACATAATCCGGCGGTAGAAAAGCGTCTGGCGGCGATCACC GCCCAGGACAGCCAGCGTGCGAATGTCTATGAAGTGCGTGCTGAAGCCCAGCGTGCGCGTTTTAAACTGCCAGCGTGGCC GACCACCACGATTGGTTCCTTCCCGCAAACCACGGAAATTCGTACCCTGCGTCTGGATTTCAAAAAGGGCAATCTCGACG CCAACAACTACCGCACGGGCATTGCGGAACATATCAAGCAGGCCATTGTTGAGCAGGAACGTTTGGGACTGGATGTGCTG GTACATGGCGAGGCCGAGCGTAATGACATGGTGGAATACTTTGGCGAGCACCTCGACGGATTTGTCTTTACGCAAAACGG TTGGGTACAGAGCTACGGTTCCCGCTGCGTGAAGCCACCGATTGTTATTGGTGACGTGAGCCGACCGGCACCGATTACCG TGGAGTGGGCGAAATATGCGCAGTCGCTGACCGACAAACCGGTGAAAGGGATGCTGACTGGTCCGGTGACGATTCTCTGC TGGTCGTTCCCGCGTGAAGATGTGAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCGGA CCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGATGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACT GGGACGCCTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACT CACATGTGTTATTGCGAGTTCAACGACATCATGGATTCCATTGCGGCGCTGGATGCGGATGTGATTACCATCGAAACTTC GCGTTCTGACATGGAGTTGCTGGAGTCGTTCGAAGAGTTTGATTATCCCAATGAAATCGGTCCAGGCGTCTATGATATTC ACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCCTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTG TGGGTTAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGC GCAGAACTTGCGTCGGGGGTAA
Upstream 100 bases:
>100_bases GCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAATACTAAA AATTCCATAAGGGGCATATA
Downstream 100 bases:
>100_bases ATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAG CATACGCTGCCAGCCATCTT
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 753; Mature: 752
Protein sequence:
>753_residues MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNV PPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQMLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVELHKRLPSDWLLSAGLINGRNVWRADLSEKYAQIKDIVGKRDLWVA SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAIT AQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILC WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHT HMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG
Sequences:
>Translated_753_residues MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNV PPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQMLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVELHKRLPSDWLLSAGLINGRNVWRADLSEKYAQIKDIVGKRDLWVA SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAIT AQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILC WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHT HMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG >Mature_752_residues TILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVP PRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPVL LGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQMLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLLT TYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVELHKRLPSDWLLSAGLINGRNVWRADLSEKYAQIKDIVGKRDLWVAS SCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITA QDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVLV HGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCW SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTH MCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLW VNPDCGLKTRGWPETRAALANMVQAAQNLRRG
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=753, Percent_Identity=98.804780876494, Blast_Score=1531, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=761, Percent_Identity=49.1458607095926, Blast_Score=715, Evalue=0.0,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 84739; Mature: 84608
Theoretical pI: Translated: 5.65; Mature: 5.65
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.3 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLP CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEE VGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC TNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPVLLGPVTYLWLGKVKGEQFDR CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCHHHH LSLLNDILPVYQQMLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHCHHHHHHHHHHHHHH TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVELHKRLPSDWLLSAGLINGRNVWRAD HHHHCCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHH LSEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR HHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHH DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR HHHCCCCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL EECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYA EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCCEEEHHHHHH QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD HHHHCCCCCCCCCHHHHHHHCCHHEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCC VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure TILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLP CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEE VGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC TNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPVLLGPVTYLWLGKVKGEQFDR CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCHHHH LSLLNDILPVYQQMLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHCHHHHHHHHHHHHHH TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVELHKRLPSDWLLSAGLINGRNVWRAD HHHHCCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHH LSEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR HHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHH DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR HHHCCCCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL EECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYA EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCCEEEHHHHHH QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD HHHHCCCCCCCCCHHHHHHHCCHHEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCC VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA