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The map label for this gene is uvrD

Identifier: 218697531

GI number: 218697531

Start: 4380389

End: 4382551

Strand: Direct

Name: uvrD

Synonym: EC55989_4287

Alternate gene names: 218697531

Gene position: 4380389-4382551 (Clockwise)

Preceding gene: 218697530

Following gene: 218697533

Centisome position: 84.98

GC content: 57.7

Gene sequence:

>2163_bases
ATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGT
GCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGCTGATGAGCGTGGAAAACTGCTCGC
CATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACC
AGCCAGGGCGGCATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGCCTGCTGCGTGCGCACCATATGGACGCCAATCT
GCCGCAGGATTTCCAGATCCTCGATAGCGAGGACCAGCTGCGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACG
AGAAGCAGTGGCCGCCGCGCCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAA
AGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACCGCGCGGGCCTGGT
GGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAACTGTGGCTTAACAAGCCGCATATCCTACAACACTACCGCGAACGTT
TTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACC
GGCAAAGTGATGATTGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTT
CCTTAATGATTTCCCCGGTGCCGAAACTATTCGTCTGGAGCAAAACTACCGCTCTACCAGCAATATTCTGAGCGCCGCTA
ACGCCCTGATTGAAAACAATAACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTC
TATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGGCAGGACAACGGCGGGGCGCT
TGCCGAGTGCGCCATTCTCTACCGCAGTAACGCCCAGTCGCGTGTACTGGAAGAAGCACTGTTGCAGGCCAGTATGCCGT
ACCGTATTTACGGCGGGATGCGCTTCTTCGAACGCCAGGAAATCAAAGACGCGCTCTCGTATCTGCGTCTGATTGCCAAC
CGTAACGACGACGCGGCGTTTGAGCGCGTGGTGAATACGCCAACGCGGGGTATTGGTGACCGGACGCTGGACGTGGTACG
TCAGACATCGCGCGATCGCCAGTTAACACTCTGGCAGGCATGTCGTGAACTGTTGCAGGAAAAAGCCCTCGCCGGGCGAG
CTGCCAGCGCCTTGCAGCGATTTATGGAATTAATCGATGCCTTAGCGCAAGAAACTGCCGATATGCCGCTGCATGTACAG
ACTGACCGGGTAATTAAAGACTCCGGCCTGCGCACCATGTACGAGCAGGAGAAGGGCGAAAAAGGCCAGACGCGTATCGA
AAACTTAGAGGAACTGGTGACGGCAACGCGCCAGTTCAGCTACAACGAAGAAGATGAAGATTTAATGCCGCTGCAGGCAT
TCCTCTCCCATGCGGCGCTGGAAGCGGGCGAGGGGCAGGCGGATACCTGGCAGGATGCAGTGCAGTTGATGACGCTGCAC
TCGGCGAAAGGCCTGGAGTTCCCGCAGGTGTTTATTGTCGGTATGGAAGAGGGTATGTTCCCAAGCCAGATGTCGCTGGA
TGAAGGCGGGCGTCTGGAAGAAGAACGCCGTCTGGCCTACGTTGGTGTAACCCGCGCGATGCAGAAACTGACGCTGACCT
ACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGT
GTGGAAGAGGTGCGCCTGCGCGCTACGGTAAGTCGTCCGGTCAGCCATCAACGGATGGGGACGCCGATGGTCGAGAACGA
CAGCGGCTACAAGCTCGGTCAGCGTGTACGCCACGCTAAGTTTGGTGAAGGCACCATCGTCAATATGGAAGGCAGCGGCG
AGCATAGCCGTTTGCAGGTGGCATTCCAGGGCCAGGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTG
TAA

Upstream 100 bases:

>100_bases
TGACCTCGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCT
ATTTTTACGCGGCGGTGCCA

Downstream 100 bases:

>100_bases
CGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCATATTCAATAAATTGCAACTTCATGTAGGCC
GGATAGGGCGTTTACGCCGC

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGT
SQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDT
GKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISL
YCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIAN
RNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQ
TDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLH
SAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEEC
VEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV

Sequences:

>Translated_720_residues
MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGT
SQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDT
GKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISL
YCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIAN
RNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQ
TDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLH
SAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEEC
VEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV
>Mature_720_residues
MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGT
SQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDT
GKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISL
YCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIAN
RNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQ
TDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLH
SAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEEC
VEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI2367296, Length=720, Percent_Identity=100, Blast_Score=1494, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=641, Percent_Identity=38.3775351014041, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=28.5294117647059, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=734, Percent_Identity=26.7029972752044, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324477, Length=693, Percent_Identity=23.2323232323232, Blast_Score=131, Evalue=4e-31,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): UVRD_ECOLI (P03018)

Other databases:

- EMBL:   X00738
- EMBL:   D00069
- EMBL:   X04037
- EMBL:   M87049
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X00225
- EMBL:   M38257
- PIR:   F65185
- RefSeq:   AP_003986.1
- RefSeq:   NP_418258.1
- PDB:   2IS1
- PDB:   2IS2
- PDB:   2IS4
- PDB:   2IS6
- PDBsum:   2IS1
- PDBsum:   2IS2
- PDBsum:   2IS4
- PDBsum:   2IS6
- ProteinModelPortal:   P03018
- SMR:   P03018
- DIP:   DIP-11103N
- IntAct:   P03018
- MINT:   MINT-1223204
- STRING:   P03018
- EnsemblBacteria:   EBESCT00000002457
- EnsemblBacteria:   EBESCT00000002458
- EnsemblBacteria:   EBESCT00000015898
- GeneID:   948347
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3786
- KEGG:   eco:b3813
- EchoBASE:   EB1057
- EcoGene:   EG11064
- eggNOG:   COG0210
- GeneTree:   EBGT00050000008889
- HOGENOM:   HBG467281
- OMA:   WRGAEVE
- ProtClustDB:   PRK11773
- BioCyc:   EcoCyc:EG11064-MONOMER
- BioCyc:   MetaCyc:EG11064-MONOMER
- Genevestigator:   P03018
- GO:   GO:0005737
- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016
- Gene3D:   G3DSA:1.10.10.160
- PANTHER:   PTHR11070
- TIGRFAMs:   TIGR01075

Pfam domain/function: PF00580 UvrD-helicase

EC number: =3.6.4.12

Molecular weight: Translated: 81991; Mature: 81991

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS51198 UVRD_HELICASE_ATP_BIND; PS51217 UVRD_HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAV
CCHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQL
EECCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHH
RLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEA
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
CDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDT
HHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCC
GKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENN
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCC
NGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQS
CCCCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHH
RVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGD
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCH
RTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQ
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAAL
CCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAY
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
VGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHCC
TPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV
CCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAV
CCHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQL
EECCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHH
RLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEA
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
CDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDT
HHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCC
GKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENN
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCC
NGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQS
CCCCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHH
RVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGD
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCH
RTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQ
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAAL
CCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAY
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
VGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHCC
TPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV
CCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285