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The map label for this gene is ilvG

Identifier: 218697486

GI number: 218697486

Start: 4330853

End: 4332499

Strand: Direct

Name: ilvG

Synonym: EC55989_4240

Alternate gene names: 218697486

Gene position: 4330853-4332499 (Clockwise)

Preceding gene: 218697485

Following gene: 218697487

Centisome position: 84.01

GC content: 54.46

Gene sequence:

>1647_bases
ATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAAT
TATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATGCCGACATGAGCAGGGTGCGGCAATGGCGG
CTATCGGTTATGCCCGTGCTACCGGCAAAACTGGCGTATGTATCGCCACGTCTGGTCCGGGCGCAACCAACCTGATAACC
GGGCTTGCGGACGCACTGTTAGATTCCATCCCTGTCGTTGCCATCACCGGTCAAGTGTCCGCACCTTTTATCGGCACCGA
CGCATTTCAGGAAGTGGATGTCCTGGGATTGTCGTTAGCCTGTACCAAGCACAGCTTTCTGGTGCAGTCGCTGGAAGAGT
TGCCGCGCATCATGGCTGCAGCATTCGACGTTGCCAGCTCAGGTCGTCCTGGTCCGGTTCTGGTCGATATCCCAAAAGAT
ATCCAGCTAGCCAGCGGTGACCTGGAACCGTGGTTCACCACCGTTGAAAACGAAGTGACTTTCCCACATGCCGAAGTTGA
GCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGTGGTGGCGTGGGTATGGCGCAGGCAGTTC
CTGCTTTACGAGAATTTCTCGCTACCACAAAAATGCCTGCCACCTGCACGCTGAAAGGGCTGGGCGCAGTTGAAGCAGAT
TATCCGTACTACCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGAT
CGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGG
ATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCA
TTACAGCAGCCGTTAAATATCAATGACTGGCAGCAATACTGCGCGCAGCTGCGTGATGAACATACCTGGCGTTACGACCA
TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAG
ATGTGGGGCAGCACCAGATGTGGGCCGCGCAGCACATCGCACACACTCGCCCGGAAAATTTCATTACCTCCAGCGGCTTA
GGCACCATGGGTTTCGGTTTACCAGCGGCGGTTGGCGCACAAGTCGCACGACCGAACGATACTGTCGTCTGTATCTCCGG
TGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCG
ATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGAT
AACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGC
ACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGG
TGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGA

Upstream 100 bases:

>100_bases
GTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGA
TTCAGGACGGGGAACTAACT

Downstream 100 bases:

>100_bases
TGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCGGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGT
TTCCACGTCTGCTCAATGAA

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIATSGPGATNLIT
GLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPA
LQQPLNINDWQQYCAQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGL
GTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
NPDFLMLASAFGIPGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS

Sequences:

>Translated_548_residues
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIATSGPGATNLIT
GLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPA
LQQPLNINDWQQYCAQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGL
GTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
NPDFLMLASAFGIPGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS
>Mature_548_residues
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIATSGPGATNLIT
GLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPA
LQQPLNINDWQQYCAQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGL
GTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
NPDFLMLASAFGIPGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=555, Percent_Identity=24.1441441441441, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI21361361, Length=498, Percent_Identity=24.8995983935743, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=46.7391304347826, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=43.8721136767318, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=29.8747763864043, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1787096, Length=541, Percent_Identity=30.3142329020333, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788716, Length=552, Percent_Identity=27.7173913043478, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=27.5922671353251, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=27.5922671353251, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=570, Percent_Identity=23.6842105263158, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=577, Percent_Identity=43.5008665511265, Blast_Score=458, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=481, Percent_Identity=23.2848232848233, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922626, Length=559, Percent_Identity=25.7602862254025, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59177; Mature: 59177

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEEHHHHHC
TGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLA
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHEEEEH
CTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKDIQLASGDLEPWFTTVENEVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEECCCCCCCHHHCCCCCCC
FPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEM
CCHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
NKLRQAHVALQGDLNALLPALQQPLNINDWQQYCAQLRDEHTWRYDHPGDAIYAPLLLKQ
HHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGLGTMGFGLPAAVGAQVARPND
HCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHCCEEECCCC
TVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
EEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
NPDFLMLASAFGIPGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASN
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
SEMLEKLS
HHHHHHCC
>Mature Secondary Structure
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEEHHHHHC
TGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLA
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHEEEEH
CTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKDIQLASGDLEPWFTTVENEVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEECCCCCCCHHHCCCCCCC
FPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEM
CCHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
NKLRQAHVALQGDLNALLPALQQPLNINDWQQYCAQLRDEHTWRYDHPGDAIYAPLLLKQ
HHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGLGTMGFGLPAAVGAQVARPND
HCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHCCEEECCCC
TVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
EEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
NPDFLMLASAFGIPGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASN
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
SEMLEKLS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]