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The map label for this gene is ubiH

Identifier: 218696503

GI number: 218696503

Start: 3277454

End: 3278632

Strand: Reverse

Name: ubiH

Synonym: EC55989_3195

Alternate gene names: 218696503

Gene position: 3278632-3277454 (Counterclockwise)

Preceding gene: 218696504

Following gene: 218696502

Centisome position: 63.6

GC content: 60.05

Gene sequence:

>1179_bases
ATGAGCGTAATCATCGTCGGTGGCGGCATGGCGGGCGCGACGCTGGCGCTGGCTATTTCCCGGTTAAGTCACGGGGCGCT
GCCGGTACATTTGATTGAAGCGACCGCGCCAGAGTCACATGCTCATCCGGGCTTTGATGGACGAGCTATTGCGCTGGCTG
CGGGGACCTGTCAGCAACTGGCGCGCATCGGCGTCTGGCAATCTCTGGCGGATTACGCAACCGCCATCACTACCGTGCAT
GTCAGCGATCGTGGTCACGCTGGATTTGTCACCCTCGCCGCAGAAGATTACCAACTGGCGGCGCTGGGACAGGTTGTCGA
ATTGCACAATGTCGGGCAACGGCTGTTTGCATTGCTGCGTAAAGCACCTGGCGTAACGCTGCATTGCCCTGATCGCGTGG
CTAACGTTGCCCGTACTCAGAGTCACGTTGAAGTGACGCTGGAGAGTGGCGAGACGCTGACGGGCCGCGTGCTGGTAGCA
GCTGATGGCACCCATTCAGCGTTAGCCACCGCGTGCGGCGTTGACTGGCAGCAGGAGCCTTACGAACAACTGGCCGTGAT
TGCCAACGTTGCGACTTCCGTTGCGCACGAAGGCCGCGCTTTTGAACGCTTCACGCAACATGGCCCGCTGGCGATGTTGC
CGATGTCTGACGGACGCTGTTCGCTGGTCTGGTGTCATCCACTGGAACGGCGCGAAGAGGTGCTGTCGTGGAGCGACGAG
AAGTTTTGCCGTGAACTCCAGTCGGCCTTTGGCTGGCGACTTGGAAAAATTACCCACGCCGGTAAACGCAGTGCTTATCC
GCTGGCGTTAACCCGCGCCGCCAGAGCTATTACCCATCGTACCGTGCTGGTGGGCAATGCGGCGCAAACTCTGCACCCGA
TTGCCGGGCAAGGGTTTAACCTCGGTATGCGAGATGTCATGAGCCTTGCGGAAACCCTGACTCAGGCGCATGAGCGCGGA
GAAGACATCGGTGATTACGGCATATTGTGCCGCTATCAGCAGCGTCGACAGAGCGATCGCGAAGCAACCATCGGCGTCAC
GGACAGCCTGGTACATCTTTTTGCCAACCGTTGGGCACCGCTGGTTGTCGGGCGCAACATCGGACTGATGACGATGGAAT
TATTCACCCCGGCACGCGATGTGCTGGCGCAGCGCACCCTCGGTTGGGTGGCGCGTTGA

Upstream 100 bases:

>100_bases
ACGACATTGTGATTACCGAAACCGGCAACGAAAACCTCACCGCCAGCGTGGTGAAAAAGCCGGAAGAAATCGAAGCGTTG
ATGGCAGCTGCGAGAAAGCA

Downstream 100 bases:

>100_bases
AGGCGGTTTTAAGGAATAAACAATGCAAAGTGTTGATGTAGCCATTGTTGGTGGCGGCATGGTGGGGCTGGCGGTTGCCT
GCGGCTTACAGGGGAGCGGC

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADYATAITTVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
KFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERG
EDIGDYGILCRYQQRRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR

Sequences:

>Translated_392_residues
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADYATAITTVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
KFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERG
EDIGDYGILCRYQQRRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
>Mature_391_residues
SVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHV
SDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVAA
DGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDEK
FCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGE
DIGDYGILCRYQQRRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=384, Percent_Identity=29.4270833333333, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI32967307, Length=374, Percent_Identity=29.9465240641711, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=98.469387755102, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=30.7888040712468, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1786883, Length=372, Percent_Identity=31.7204301075269, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI25146631, Length=425, Percent_Identity=26.1176470588235, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321694, Length=453, Percent_Identity=24.9448123620309, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920760, Length=421, Percent_Identity=23.9904988123515, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42357; Mature: 42226

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
CEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVAADGTHSALATACGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEEECCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCHH
KFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHCEECCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQRRQSDREATIGVTDSLVHLFANRWAP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEHHHCCCCCCEECCHHHHHHHHHHCCCCE
LVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
EEEECCCCEEEHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
EEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVAADGTHSALATACGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEEECCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCHH
KFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHCEECCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQRRQSDREATIGVTDSLVHLFANRWAP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEHHHCCCCCCEECCHHHHHHHHHHCCCCE
LVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
EEEECCCCEEEHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]