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The map label for this gene is hycG

Identifier: 218696313

GI number: 218696313

Start: 3057067

End: 3057834

Strand: Reverse

Name: hycG

Synonym: EC55989_2985

Alternate gene names: 218696313

Gene position: 3057834-3057067 (Counterclockwise)

Preceding gene: 218696314

Following gene: 218696312

Centisome position: 59.32

GC content: 57.16

Gene sequence:

>768_bases
ATGAGCAATTTATTAGGCCCCCGTGACGCCAACGGCATTCCGGTCCCGATGACGGTGGATGAATCCATCGCCAGCATGAA
GGCGTCGTTACTGAAAAAAATCAAACGTTCTGCCTACGTTTACCGCGTGGACTGCGGCGGCTGCAACGGCTGCGAAATCG
AAATTTTCGCCACGCTTTCGCCGCTGTTTGATGCAGAACGCTTCGGCATTAAAGTCGTTCCTTCACCGCGTCATGCGGAT
ATTTTACTGTTTACCGGCGCGGTCACCCGTGCAATGCGATCCCCTGCGCTGTGTGCGTGGCAGTCCGCGCCGGACCCGAA
AATCTGTATCTCCTACGGTGCCTGCGGTAACAGTGGCGGGATCTTCCACGATCTCTACTGCGTGTGGGGCGGTACGGATA
AAATCGTCCCGGTAGATGTCTACATCCCTGGTTGCCCGCCAACGCCTGCCGCCACGCTGTACGGCTTTGCAATGGCGCTC
GGCCTGCTGGAGCAGAAAATTCACGCCCGTGGCCCGGGTGAGCAGGATGAACAACCGGCGGAAATCCTGCATGGCGATAT
GGTGCAGCCGCTGCGTGTGAAAGTGGATCGCGAAGCACGCCGCCTGGCAGGTTATCGTTACGGTCGTCAGATTGCCGATG
ACTTCCTGACACAGTTAGGGCAGGGCGAAGAACAGGTTGCACGCTGGCTGGAAGCGGAAAACGATCCGCGTCTGAACGAG
ATTGTCAGCCATCTGAATCATGTTGTAGAAGAGGCGCGTATCCGATGA

Upstream 100 bases:

>100_bases
ACCGCGAAAGCTTTGAGACTTGCCCGGAATGTAAGCGCCAGAAATGCCTGGTGCCGTCCGACCGTATTGAACTGACTCGC
CATATGAAAGAGGCCATCTG

Downstream 100 bases:

>100_bases
GTGAAAAGGTGGTGTTCAGTCAACTGAGCCGTAAATTTATTGATGAGAACGATGCCACGCCCGCCGAGGCGCAGCAGGTG
GTCTATTACAGCCTGGCGAT

Product: hydrogenase 3 and formate hydrogenase complex, HycG subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHAD
ILLFTGAVTRAMRSPALCAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQLGQGEEQVARWLEAENDPRLNE
IVSHLNHVVEEARIR

Sequences:

>Translated_255_residues
MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHAD
ILLFTGAVTRAMRSPALCAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQLGQGEEQVARWLEAENDPRLNE
IVSHLNHVVEEARIR
>Mature_254_residues
SNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADI
LLFTGAVTRAMRSPALCAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALG
LLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQLGQGEEQVARWLEAENDPRLNEI
VSHLNHVVEEARIR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=111, Percent_Identity=43.2432432432432, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789074, Length=255, Percent_Identity=98.8235294117647, Blast_Score=519, Evalue=1e-149,
Organism=Escherichia coli, GI1788834, Length=251, Percent_Identity=61.7529880478088, Blast_Score=304, Evalue=3e-84,
Organism=Escherichia coli, GI1788624, Length=157, Percent_Identity=36.3057324840764, Blast_Score=94, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=125, Percent_Identity=39.2, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859983, Length=110, Percent_Identity=41.8181818181818, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24642371, Length=110, Percent_Identity=41.8181818181818, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24651058, Length=109, Percent_Identity=41.2844036697248, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 27945; Mature: 27814

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLS
CCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECC
PLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALCAWQSAPDPKICISYGACGNSGG
HHHHHHHCCEEEECCCCCCEEEEEHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCC
IFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALGLLEQKIHARGPGEQDEQPA
CEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQLGQGEEQVARWLEAENDPRLNE
HHHHCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
IVSHLNHVVEEARIR
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLS
CCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECC
PLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALCAWQSAPDPKICISYGACGNSGG
HHHHHHHCCEEEECCCCCCEEEEEHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCC
IFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALGLLEQKIHARGPGEQDEQPA
CEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQLGQGEEQVARWLEAENDPRLNE
HHHHCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
IVSHLNHVVEEARIR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]