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The map label for this gene is srlD

Identifier: 218696296

GI number: 218696296

Start: 3038741

End: 3039520

Strand: Direct

Name: srlD

Synonym: EC55989_2967

Alternate gene names: 218696296

Gene position: 3038741-3039520 (Clockwise)

Preceding gene: 218696295

Following gene: 218696297

Centisome position: 58.95

GC content: 52.95

Gene sequence:

>780_bases
ATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTA
TCGCGTCGCGGTTGTCGATATTCAGAGCGACAAAGCCGCAAATGTGGCACAAGAAATTAACGCCGAATATGGTGAAAGTA
TGGCGTACGGTTTTGGTGCTGACGCCACTAGCGAGCAAAGCGTTCTGGCGCTCTCTCGTGGGGTAGATGAAATCTTTGGT
CGCGTGGATTTGCTGGTCTATAGCGCCGGAATAGCAAAAGCAGCCTTTATCAGCGACTTCCAGCTCGGCGATTTTGACCG
TTCGCTACAAGTGAATCTGGTGGGTTATTTCCTGTGTGCCCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGG
GTCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGT
GGCGTCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCT
GCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGT
ATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCT
AAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGA

Upstream 100 bases:

>100_bases
CGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTACCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTG
AATCTGTTAAGGAGTAAAAA

Downstream 100 bases:

>100_bases
TCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTT
CCGCCACTACATTAAGGAAA

Product: sorbitol-6-phosphate dehydrogenase

Products: NA

Alternate protein names: Glucitol-6-phosphate dehydrogenase; Ketosephosphate reductase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQEINAEYGESMAYGFGADATSEQSVLALSRGVDEIFG
RVDLLVYSAGIAKAAFISDFQLGDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDKVPLKRGCDYQDVLNMLLFYASP
KASYCTGQSINVTGGQVMF

Sequences:

>Translated_259_residues
MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQEINAEYGESMAYGFGADATSEQSVLALSRGVDEIFG
RVDLLVYSAGIAKAAFISDFQLGDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDKVPLKRGCDYQDVLNMLLFYASP
KASYCTGQSINVTGGQVMF
>Mature_259_residues
MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQEINAEYGESMAYGFGADATSEQSVLALSRGVDEIFG
RVDLLVYSAGIAKAAFISDFQLGDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDKVPLKRGCDYQDVLNMLLFYASP
KASYCTGQSINVTGGQVMF

Specific function: Sorbitol metabolism. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family

Homologues:

Organism=Homo sapiens, GI15277342, Length=265, Percent_Identity=32.8301886792453, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4758504, Length=233, Percent_Identity=32.1888412017167, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI40254992, Length=256, Percent_Identity=27.34375, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI19923817, Length=266, Percent_Identity=26.6917293233083, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI83715985, Length=233, Percent_Identity=30.9012875536481, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI59889578, Length=231, Percent_Identity=27.7056277056277, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789057, Length=259, Percent_Identity=100, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1787335, Length=256, Percent_Identity=28.90625, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787905, Length=255, Percent_Identity=29.0196078431373, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082160, Length=259, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788459, Length=264, Percent_Identity=28.4090909090909, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI2367175, Length=257, Percent_Identity=29.9610894941634, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=27.6264591439689, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790717, Length=253, Percent_Identity=22.5296442687747, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789208, Length=257, Percent_Identity=26.4591439688716, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=28.740157480315, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25147288, Length=254, Percent_Identity=30.3149606299213, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508895, Length=169, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17538182, Length=228, Percent_Identity=32.0175438596491, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=270, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=219, Percent_Identity=29.2237442922374, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=243, Percent_Identity=25.9259259259259, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=269, Percent_Identity=24.1635687732342, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=243, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=211, Percent_Identity=31.7535545023697, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24639444, Length=255, Percent_Identity=28.2352941176471, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI17737361, Length=233, Percent_Identity=30.9012875536481, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355631, Length=170, Percent_Identity=30, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357041, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI23397609, Length=259, Percent_Identity=25.8687258687259, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24581726, Length=194, Percent_Identity=23.1958762886598, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SRLD_ECOLI (P05707)

Other databases:

- EMBL:   J02708
- EMBL:   U29579
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E65050
- RefSeq:   AP_003272.1
- RefSeq:   NP_417185.1
- ProteinModelPortal:   P05707
- SMR:   P05707
- DIP:   DIP-10917N
- STRING:   P05707
- EnsemblBacteria:   EBESCT00000003950
- EnsemblBacteria:   EBESCT00000018220
- GeneID:   948937
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2674
- KEGG:   eco:b2705
- EchoBASE:   EB0964
- EcoGene:   EG10971
- eggNOG:   COG1028
- GeneTree:   EBGT00070000031731
- HOGENOM:   HBG750976
- OMA:   SEEASYC
- ProtClustDB:   PRK12384
- BioCyc:   EcoCyc:SORB6PDEHYDROG-MONOMER
- BioCyc:   MetaCyc:SORB6PDEHYDROG-MONOMER
- Genevestigator:   P05707
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081
- PRINTS:   PR00080

Pfam domain/function: PF00106 adh_short

EC number: =1.1.1.140

Molecular weight: Translated: 27858; Mature: 27858

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 154-154 BINDING 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQEINAEYGESMAYGFGA
CCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEECCCC
DATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQLGDFDRSLQVNLVGYFLCA
CCCCCHHHHHHHCCHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCCCCEEHHHHHHHHHH
REFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFGGVGLTQSLALDLAEYGITVH
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCHHHH
SLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDKVPLKRGCDYQDVLNMLLFYASP
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHCCC
KASYCTGQSINVTGGQVMF
CCCCCCCCEEECCCCEEEC
>Mature Secondary Structure
MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQEINAEYGESMAYGFGA
CCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEECCCC
DATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQLGDFDRSLQVNLVGYFLCA
CCCCCHHHHHHHCCHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCCCCEEHHHHHHHHHH
REFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFGGVGLTQSLALDLAEYGITVH
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCHHHH
SLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDKVPLKRGCDYQDVLNMLLFYASP
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHCCC
KASYCTGQSINVTGGQVMF
CCCCCCCCEEECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3553176; 9205837; 9278503; 6384188