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The map label for this gene is nrdF

Identifier: 218696273

GI number: 218696273

Start: 3014190

End: 3015149

Strand: Direct

Name: nrdF

Synonym: EC55989_2944

Alternate gene names: 218696273

Gene position: 3014190-3015149 (Clockwise)

Preceding gene: 218696272

Following gene: 218696274

Centisome position: 58.47

GC content: 48.12

Gene sequence:

>960_bases
ATGAAACTCTCACGTATCAGCGCCATCAACTGGAACAAGATATCTGACGATAAAGATCTGGAGGTGTGGAATCGCCTGAC
CAGCAATTTCTGGCTACCAGAAAAGGTGCCGCTGTCGAACGATATTCCTGCCTGGCAGACATTAACTGTCGTAGAACAAC
AACTGACGATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCGCTGATGCCC
GATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTC
GATTTTCTCGACGCTGTGTCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAA
AAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTT
TTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCAGATCTGATCCGTCT
GATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGAC
AACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTG
TACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTA
CGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATC
ACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGA

Upstream 100 bases:

>100_bases
CAAAGGTATCAAAACGCTCTATTACATTCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTCTCCT
GTGCACTTTAAGGAATATCT

Downstream 100 bases:

>100_bases
GGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATATTCT
GTAGTGATCTACACTGATAC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
DALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF

Sequences:

>Translated_319_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
DALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF
>Mature_319_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
DALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family

Homologues:

Organism=Escherichia coli, GI1789031, Length=319, Percent_Identity=100, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI1788567, Length=265, Percent_Identity=24.9056603773585, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIR4_ECOLI (P37146)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M24856
- PIR:   E65047
- RefSeq:   AP_003249.1
- RefSeq:   NP_417162.1
- PDB:   3N37
- PDB:   3N38
- PDB:   3N39
- PDB:   3N3A
- PDB:   3N3B
- PDBsum:   3N37
- PDBsum:   3N38
- PDBsum:   3N39
- PDBsum:   3N3A
- PDBsum:   3N3B
- ProteinModelPortal:   P37146
- SMR:   P37146
- DIP:   DIP-374N
- STRING:   P37146
- EnsemblBacteria:   EBESCT00000000726
- EnsemblBacteria:   EBESCT00000015330
- GeneID:   947149
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2651
- KEGG:   eco:b2676
- EchoBASE:   EB2283
- EcoGene:   EG12381
- eggNOG:   COG0208
- GeneTree:   EBGT00050000010239
- HOGENOM:   HBG516660
- OMA:   TLCSTPD
- ProtClustDB:   PRK09614
- BioCyc:   EcoCyc:NRDF-MONOMER
- BioCyc:   MetaCyc:NRDF-MONOMER
- Genevestigator:   P37146
- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358
- Gene3D:   G3DSA:1.10.620.20
- PANTHER:   PTHR23409

Pfam domain/function: PF00268 Ribonuc_red_sm; SSF47240 Ferritin/RR_like

EC number: =1.17.4.1

Molecular weight: Translated: 36443; Mature: 36443

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00368 RIBORED_SMALL

Important sites: ACT_SITE 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLTN
HEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCH
TADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
HHHHHHHHHHHCCCCEEEEEEEHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGS
HHCCCCHHHHHHHEEECCCHHHHHCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCC
GSSYVMGKAVETEDEDWNF
CCCEEEECEECCCCCCCCC
>Mature Secondary Structure
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLTN
HEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCH
TADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
HHHHHHHHHHHCCCCEEEEEEEHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGS
HHCCCCHHHHHHHEEECCCHHHHHCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCC
GSSYVMGKAVETEDEDWNF
CCCEEEECEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2649479; 8195103