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The map label for this gene is yfiN

Identifier: 218696228

GI number: 218696228

Start: 2965631

End: 2966857

Strand: Direct

Name: yfiN

Synonym: EC55989_2893

Alternate gene names: 218696228

Gene position: 2965631-2966857 (Clockwise)

Preceding gene: 218696227

Following gene: 218696229

Centisome position: 57.53

GC content: 48.41

Gene sequence:

>1227_bases
ATGATGGATAACGATAATTCTCTTAATAAGCGCCCCACGTTTAAAAGAGCATTACGCAACATCAGTATGACCAGCATATT
TATCACTATGATGCTGATCTGGTTGCTGCTTTCCGTGACCTCGGTGCTGACCCTGAAACAGTACGCGCAAAAAAACCTGG
CACTGACAGCAGCAACAATGACTTACAGTCTGGAAGCAGCTGTCGTTTTTGCCGATGGCCCTGCAGCAACTGAAACACTG
GCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTA
CACCCGTAAGGATCCAGGCGATACTTTCAGCAATTTCATAAGCCACTGGCTCTTTCCTGCCCCCATCATTCAGCCGATTC
GTCACAATGGCGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTC
GCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGAATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTGGA
AGCACTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTA
TCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTACAGGCT
AAAAATGCGCAGCTTCTACGTACCGCGCTACATGACCCATTAACCGGGCTGGCTAACCGCGCAGCGTTTCGTAGCGGCAT
CAACACGTTGATGAACAATTCCGATGCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCA
ATGATACCTGGGGTCATGCGACGGGCGATAGGGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGA
CATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCAGAATCTGAAGTGCAGCAGAT
ATGCTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTG
GTTACGCGATGACCATTGAGCACGCCTCTGCGGAAAAATTACAAGAGCTTGCCGATCACAATATGTATCAGGCCAAACAC
CAGCGTGCCGAAAAGCTGCTGAGATAA

Upstream 100 bases:

>100_bases
ATCCACAATAATGACGTCAGATTTGCCGTAAACCTGGATGCCTTATCGCGTAGCGGTGTAAAGGTCAACCCGGATGTCTT
AATGCTCGCACGGAAGAAAA

Downstream 100 bases:

>100_bases
CAAGGATATATCGATATGATAAAGCACCTGGTAGCACCCCTGATTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCC
TCAGGGAAAGTTTACTCCTG

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATETL
AALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLR
HKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKH
QRAEKLLR

Sequences:

>Translated_408_residues
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATETL
AALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLR
HKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKH
QRAEKLLR
>Mature_408_residues
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATETL
AALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLR
HKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKH
QRAEKLLR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=408, Percent_Identity=99.7549019607843, Blast_Score=843, Evalue=0.0,
Organism=Escherichia coli, GI145693134, Length=153, Percent_Identity=32.6797385620915, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787541, Length=152, Percent_Identity=35.5263157894737, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=32.0512820512821, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788381, Length=100, Percent_Identity=42, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=28.8343558282209, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=31.1764705882353, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786584, Length=150, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=101, Percent_Identity=41.5841584158416, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46004; Mature: 46004

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATM
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHH
TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFI
EEEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRH
HHHCCCHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCC
TGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFD
CCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
LHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLLR
CCCCCCEEEEEEECEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATM
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHH
TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFI
EEEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRH
HHHCCCHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCC
TGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFD
CCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
LHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLLR
CCCCCCEEEEEEECEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]