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The map label for this gene is pheA

Identifier: 218696223

GI number: 218696223

Start: 2960993

End: 2962153

Strand: Direct

Name: pheA

Synonym: EC55989_2888

Alternate gene names: 218696223

Gene position: 2960993-2962153 (Clockwise)

Preceding gene: 218696222

Following gene: 218696226

Centisome position: 57.44

GC content: 48.84

Gene sequence:

>1161_bases
ATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCATTACTGGCAGA
GCGGCGCGAATTGGCCGTCGAGGTAGGAAAAGCCAAACTGCTCTCGCATCGCCCGGTACGAGATATTGATCGTGAACGCG
ATTTACTGGAAAGATTAATTACGCTCGGTAAAGCGCACCATCTGGACGCCCATTACATTACTCGCCTGTTCCAGCTCATC
ATTGAAGATTCCGTATTAACTCAGCAGGCTTTGCTCCAGCAACATCTCAATAAAATTAATCCGCACTCAGCACGCATCGC
TTTTCTCGGCCCCAAAGGTTCTTATTCCCATCTTGCGGCGCGCCAGTATGCTGCCCGTCACTTTGAGCAATTCATTGAAA
GTGGCTGCGCCAAATTTGCCGATATTTTTAATCAGGTGGAAACCGGCCAGGCCGACTATGCCGTCGTACCGATTGAAAAT
ACCAGCTCCGGTGCCATAAACGACGTTTACGATCTGCTGCAACATACCAGCTTGTCGATTGTTGGCGAGATGACGTTAAC
TATCGACCATTGTTTGTTGGTCTCCGGCACGACTGATTTATCCACCATTAATACGGTCTACAGCCATCCGCAGCCATTCC
AGCAATGCAGCAAATTCCTTAATCGTTATCCTCACTGGAAGATTGAATATACCGAAAGTACGTCTGCGGCAATGGAAAAG
GTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGA
GCGTATTGAAGCGAATCAGCAACAAAACTTCACCCGATTTGTGGTGTTGGCACGTAAAGCCATTAACGTGTCTGACCAGG
TTCCGGCGAAAACGACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTGCTGCGCAAC
CACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTTTATCTGGATATTCA
GGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATTACCCGTTCAATGAAGGTATTGGGCT
GTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGA

Upstream 100 bases:

>100_bases
GAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGCCTTTTTTATT
GATAACAAAAAGGCAACACT

Downstream 100 bases:

>100_bases
TGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGGTTGTCATTCGCCTGACGCAATAACACGC
GGCTTTCACTCTGAAAACGC

Product: bifunctional chorismate mutase/prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLI
IEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRN
HNLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT

Sequences:

>Translated_386_residues
MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLI
IEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRN
HNLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT
>Mature_385_residues
TSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLII
EDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENT
SSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEKV
AQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNH
NLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=386, Percent_Identity=99.7409326424871, Blast_Score=798, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324013, Length=323, Percent_Identity=26.625386996904, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 43084; Mature: 42953

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAA
HHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
RQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENTSSGAINDVYDLLQHTSLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHH
VGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
HHHHHEEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQNFTRFVVLARKAINVSDQVPAKTTL
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEE
LMATGQQAGALVEALLVLRNHNLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALK
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHEEEEEEECCCCHHHHHHHHH
ELGEITRSMKVLGCYPSENVVPVDPT
HHHHHHHHHEEEEECCCCCEEECCCC
>Mature Secondary Structure 
TSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAA
HHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
RQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENTSSGAINDVYDLLQHTSLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHH
VGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
HHHHHEEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQNFTRFVVLARKAINVSDQVPAKTTL
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEE
LMATGQQAGALVEALLVLRNHNLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALK
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHEEEEEEECCCCHHHHHHHHH
ELGEITRSMKVLGCYPSENVVPVDPT
HHHHHHHHHEEEEECCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]