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The map label for this gene is clcB

Identifier: 218695154

GI number: 218695154

Start: 1814230

End: 1815486

Strand: Direct

Name: clcB

Synonym: EC55989_1757

Alternate gene names: 218695154

Gene position: 1814230-1815486 (Clockwise)

Preceding gene: 218695153

Following gene: 218695158

Centisome position: 35.19

GC content: 54.42

Gene sequence:

>1257_bases
ATGTTCCGCCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCT
GCTACTGGAGTGGTTGTTCCTCAATAATGACTCCGGCAGTCTGGTCAATGCAGCGACAAACCTTTCCCCCTGGCGACGGT
TGCTAACTCCGGCGCTCGGCGGACTGGCGGCGGGTTTGTTGCTGATGGGCTGGCAGAAATTTACCCAACAACGCCCTCAT
GCGCCGACCGATTACATGGAAGCGTTGCAAACCGATGGACAGTTCGATTACGCAGCAAGCCTGGTTAAATCGCTTGCCTC
GCTGCTGGTAGTAACCAGCGGCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTT
TTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGCCGCGGCGGGAATGGCTGCGGCCTAT
CGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGAT
TATTTCCGCCGTCGTGGCATTGCTGGTTAGCAATCTGATTAATCATAGCGACGCGTTACTCTACAACGTACAACTCTCAG
TGACGGTTCAGGCTCGTGACTATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACG
TTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGT
GGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCAC
TGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGT
GTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGG
CGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGA
CGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATT
TCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAA

Upstream 100 bases:

>100_bases
GCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCACATTTCTGCTCATGCATCATCTACACA
TCTATCCGGATCTGCGCACT

Downstream 100 bases:

>100_bases
GCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATAAGGCAGTTCACCAATGAGCGGTGCCGGAAGTTTTT
TACCCAGCACATCAATGATT

Product: putative voltage-gated ClC-type chloride channel ClcB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MFRRLLIATVVGILAAFAVAGFRHAMLLLEWLFLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPH
APTDYMEALQTDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWIACGAAAGMAAAY
RAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDALLYNVQLSVTVQARDYALIISTGVLAGLCGPLLLT
LMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGG
VFTPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVI
SRTLHRDSIYRQHTAQHS

Sequences:

>Translated_418_residues
MFRRLLIATVVGILAAFAVAGFRHAMLLLEWLFLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPH
APTDYMEALQTDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWIACGAAAGMAAAY
RAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDALLYNVQLSVTVQARDYALIISTGVLAGLCGPLLLT
LMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGG
VFTPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVI
SRTLHRDSIYRQHTAQHS
>Mature_418_residues
MFRRLLIATVVGILAAFAVAGFRHAMLLLEWLFLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPH
APTDYMEALQTDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWIACGAAAGMAAAY
RAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDALLYNVQLSVTVQARDYALIISTGVLAGLCGPLLLT
LMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGG
VFTPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVI
SRTLHRDSIYRQHTAQHS

Specific function: Probably acts as an electrical shunt for an outwardly- directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily

Homologues:

Organism=Escherichia coli, GI87081943, Length=418, Percent_Identity=99.7607655502392, Blast_Score=826, Evalue=0.0,
Organism=Escherichia coli, GI1786350, Length=263, Percent_Identity=28.1368821292776, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLCB_ECO24 (A7ZM51)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462880.1
- ProteinModelPortal:   A7ZM51
- SMR:   A7ZM51
- STRING:   A7ZM51
- EnsemblBacteria:   EBESCT00000020157
- GeneID:   5589110
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1800
- eggNOG:   COG0038
- GeneTree:   EBGT00050000010040
- HOGENOM:   HBG437193
- OMA:   SAPFAYA
- ProtClustDB:   PRK01610
- BioCyc:   ECOL331111:ECE24377A_1800-MONOMER
- HAMAP:   MF_01203
- InterPro:   IPR014743
- InterPro:   IPR001807
- Gene3D:   G3DSA:1.10.3080.10
- PANTHER:   PTHR11689
- PRINTS:   PR00762

Pfam domain/function: PF00654 Voltage_CLC; SSF81340 Cl-channel_core

EC number: NA

Molecular weight: Translated: 44173; Mature: 44173

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x159652a4)-; HASH(0x19378e68)-; HASH(0x1835505c)-; HASH(0x166ec658)-; HASH(0x19a22218)-; HASH(0x19b188b0)-; HASH(0x1885aa90)-; HASH(0x1930c558)-; HASH(0x409c126c)-; HASH(0x19793024)-;

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRLLIATVVGILAAFAVAGFRHAMLLLEWLFLNNDSGSLVNAATNLSPWRRLLTPALG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHH
GLAAGLLLMGWQKFTQQRPHAPTDYMEALQTDGQFDYAASLVKSLASLLVVTSGSAIGRE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GAMILLAALAASCFAQRFTPRQEWKLWIACGAAAGMAAAYRAPLAGSLFIAEVLFGTMML
HHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ASLGPVIISAVVALLVSNLINHSDALLYNVQLSVTVQARDYALIISTGVLAGLCGPLLLT
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCEEEEHHHHHHHHHHHHHHHH
LMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAG
HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
IFLCKLCAVLASSGSGAPGGVFTPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHH
TLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MFRRLLIATVVGILAAFAVAGFRHAMLLLEWLFLNNDSGSLVNAATNLSPWRRLLTPALG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHH
GLAAGLLLMGWQKFTQQRPHAPTDYMEALQTDGQFDYAASLVKSLASLLVVTSGSAIGRE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GAMILLAALAASCFAQRFTPRQEWKLWIACGAAAGMAAAYRAPLAGSLFIAEVLFGTMML
HHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ASLGPVIISAVVALLVSNLINHSDALLYNVQLSVTVQARDYALIISTGVLAGLCGPLLLT
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCEEEEHHHHHHHHHHHHHHHH
LMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAG
HHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
IFLCKLCAVLASSGSGAPGGVFTPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHH
TLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA