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The map label for this gene is ynfE

Identifier: 218695149

GI number: 218695149

Start: 1806984

End: 1809410

Strand: Direct

Name: ynfE

Synonym: EC55989_1752

Alternate gene names: 218695149

Gene position: 1806984-1809410 (Clockwise)

Preceding gene: 218695148

Following gene: 218695150

Centisome position: 35.05

GC content: 50.56

Gene sequence:

>2427_bases
ATGTCCAAAAATGACCGAATGGTGGGCATCAGCCGCAGAACACTCGTTAAATCAACCGCGATAGGTTCTCTGGCGCTGGC
TGCAGGCGGTTTTTCTTTACCGTTCACCCTGCGCAGTGCAGCGGCAGCGGTACAACAGGCCAGCGAAAAAGTGATCTGGG
GTGCCTGTTCCGTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGACCTGGGTGGAAACT
GACAATACCGGCAGCGATGAGTACGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCGCCGGCGTATTAA
TCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGTACACGCGGTGAAGGCAAATTCGAACGGATTAGCTGGGATG
AAGCCCTGGATACTATCGCCAGTAGCCTGAAAAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCT
TCGGGGATTGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGG
TTCACTCAATCAGTATGGCTCCTACAGCACCGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCA
ACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCGGCGGT
GGCATCACTTATCTTCTTGAAAAAGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATAC
GGCTGCTGGTCGTGAAGATGAATGGCTCCCTATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTAT
TGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGAT
GCACCCAAAAATGGTCACTATAAAGCCTATATTCTTGGTGAAGGTGATGATAACACAGCGAAAACGCCGCAGTGGGCTTC
GCAAATTACCGGTATCCCGGTAGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCC
AGGGCTGGGGGCCGCAACGCCAGGCAAACGGCGAACTGACTGCACGCGCTATTGCTATGTTACCTATTTTGACGGGCAAT
GTCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAACGCCTGCCGGTGCTGGATAA
TCCGGTCAAAACGTCAATCTCCTGCTTCAGCTGGACAGATGCTATCGATCATGGTCCGCAAATGACGGCAATCCGCGACG
GCGTCCGCGGCAAAGATAAACTGGATGTGTCCATTAAGTTCATCTGGAACTACGCGGGAAATACCCTCGTTAATCAGCAT
TCTGACATCAACAAAACGCATGAAATTCTGCAAGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGAC
CTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATTATTCCTAACGACTACG
CCGGTAACATGGGATATCTCATTTTCCTCCAGCCAGTCACCAGCGAAAAATTCGAACGTAAACCGATTTACTGGATCCTG
AGTGAAGTCGCGAAACGTCTTGGACCTGACGTCTATCAAAAGTTCACTGAAGGTCGCACGCAGGAACAATGGTTACAACA
TCTGTACGCCAAAATGCTTGCCAAAGATCCGGCGTTACCGTCTTACGACGAACTGAAAAAAATGGGTATCTATAAGCGTA
AAGATCCCAATGGTCATTTTGTCGCCTACAAAGCATTTCGTGACGACCCCGAGGCAAATCCACTTAAAACACCTTCCGGT
AAGATTGAAATTTATTCCAGCAAGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCACT
GCCAGTTTATGCCTCTACCTTTGAAGGCTGGGACTCCCCTGAACGTAGAACCTTCCCACTTCAACTGTTTGGTTTCCATT
ACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCT
ATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGC
CAAAGTGACACCACGCATTCTCCCTGGGGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATA
AAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACT
AATCTGGTCGAGATCGAAAAAATATAA

Upstream 100 bases:

>100_bases
ATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACATTTATATAACGA
TAAAGAACAGGGAGTGAGTT

Downstream 100 bases:

>100_bases
CCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGA
GGCGCTCATGAAGGCTGAAA

Product: oxidoreductase subunit

Products: NA

Alternate protein names: DMSO reductase [H]

Number of amino acids: Translated: 808; Mature: 807

Protein sequence:

>808_residues
MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVET
DNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVSIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
KIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINP
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI

Sequences:

>Translated_808_residues
MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVET
DNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVSIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
KIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINP
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI
>Mature_807_residues
SKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETD
NTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSS
GIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGGG
ITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADA
PKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNV
GISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVSIKFIWNYAGNTLVNQHS
DINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILS
EVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGK
IEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPI
DAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTN
LVEIEKI

Specific function: Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=808, Percent_Identity=98.7623762376238, Blast_Score=1667, Evalue=0.0,
Organism=Escherichia coli, GI171474008, Length=801, Percent_Identity=70.4119850187266, Blast_Score=1224, Evalue=0.0,
Organism=Escherichia coli, GI87081797, Length=805, Percent_Identity=67.2049689440994, Blast_Score=1136, Evalue=0.0,
Organism=Escherichia coli, GI145693196, Length=753, Percent_Identity=30.2788844621514, Blast_Score=289, Evalue=4e-79,
Organism=Escherichia coli, GI87081994, Length=848, Percent_Identity=29.0094339622642, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1787231, Length=760, Percent_Identity=29.3421052631579, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI3868721, Length=793, Percent_Identity=24.2118537200504, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI3868720, Length=457, Percent_Identity=26.0393873085339, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787741, Length=429, Percent_Identity=25.4079254079254, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI3868719, Length=455, Percent_Identity=23.956043956044, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788534, Length=889, Percent_Identity=22.384701912261, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011888
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 89577; Mature: 89445

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVIWGACSVNC
CCCCCCEEECHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCHHHCC
TRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNMTRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCHHCCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARESTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVSIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCEEEEEEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINP
HHCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEECCCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEKVIWGACSVNC
CCCCCEEECHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCHHHCC
TRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNMTRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCHHCCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARESTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVSIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCEEEEEEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINP
HHCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEECCCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]