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The map label for this gene is ycjW

Identifier: 218694895

GI number: 218694895

Start: 1524688

End: 1525686

Strand: Reverse

Name: ycjW

Synonym: EC55989_1484

Alternate gene names: 218694895

Gene position: 1525686-1524688 (Counterclockwise)

Preceding gene: 218694899

Following gene: 218694879

Centisome position: 29.6

GC content: 45.45

Gene sequence:

>999_bases
ATGTCCCCTACTATTTATGATATTGCCAGGGTTGCAGGCGTATCAAAATCCACCGTATCACGCGTGCTGAATAAGCAAAC
TAATATCTCCCCGGAAGCGCGCGAAAAAGTGTTACGGGCCATTGAAGAATTACAGTATCAGCCAAACAAGCTAGCCCGCG
CGCTGACCTCTTCGGGTTTTGATGCCATTATGGTGATTTCTACCCGTTCGACCAAAACTACGGCGGGTAATCCGTTTTTC
TCTGAAGTTTTGCATGCCATCACCGCCAAAGCTGAAGAGGAAGGTTTCGACGTGATATTGCAGACGTCGCACAATCCGGC
AGAAGACTTACAAAAATGTGAAAGCAAAATTAAGCAGAAAATGATTAAAGGCATTATTATGCTCAGTTCGCCAGCGGATG
AGTCATTTTTTGCCCAACTCGATAAATACGATATTCCGGTGGTGGTAATTGGCAAAGTTGAAGGTCAATATGCCCATGTT
TATTCTGTCGATACCGATAATTTTGGCGACAGCATTGCGTTGACCGATGCATTAATTGAAAGTGGGCATCAAAATATTGC
CTGCCTGCATGCTCCGCTTGATGTCCATGTTTCGGTTGATCGCGTTAATGGCTATAAGCAAAGTCTGTCCGCGCATAATA
TTGCAGTGCGTGATAAATGGATTGTTGACGGCGGTTATACCCATGAAACAGCACTGCAAGCAGCACGGCAATTATTAAGT
CAGTCACCGTTGCCTGAGGCAGTGTTTGCCACTGACAGCCTGAAATTAATGAGCATTTATCGTGCGGCGGCAGAGAAAAA
TATTGCTATTCCGCAGCAGTTAGCGGTGGTGGGTTATAGTAATGAAACGCTGTCGTTTATTTTAACGCCTGCACCAGGCG
GCATCGATGTTCCGACGCAGGAGTTAGGTCAACAAAGCTGCGAGTTATTATTCCGCTTAATTTCTGGAAAACCGTCACCA
CAGAATATTACCGTTGCCACGCATATGACGCTGAAATAA

Upstream 100 bases:

>100_bases
TGTGGGGGCCAGGCCATTGATTATCAACTGCCCGTGTTGTCATCGACGGGCGAACTCCATAAGATGTGCAGCATTCACGT
TCGGACGGAGTTCCTGACCT

Downstream 100 bases:

>100_bases
ATACCAGCTACCTTGCCTGAATGCCGGATGCGATGCTAGCGCATCTTATCCGGCCTACATTTATAGATCGGATAAAGTAC
TCGCGCCGCATCCGGCAATT

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRDKWIVDGGYTHETALQAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSP
QNITVATHMTLK

Sequences:

>Translated_332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRDKWIVDGGYTHETALQAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSP
QNITVATHMTLK
>Mature_331_residues
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFS
EVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVY
SVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRDKWIVDGGYTHETALQAARQLLSQ
SPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQ
NITVATHMTLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=99.0963855421687, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=324, Percent_Identity=30.8641975308642, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=30.3514376996805, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789068, Length=323, Percent_Identity=29.1021671826625, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=27.2151898734177, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=28.75, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=25.5520504731861, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789202, Length=320, Percent_Identity=26.5625, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786540, Length=329, Percent_Identity=25.2279635258359, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787906, Length=313, Percent_Identity=26.1980830670927, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786268, Length=289, Percent_Identity=23.8754325259516, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790689, Length=330, Percent_Identity=27.5757575757576, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=21.0526315789474, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36150; Mature: 36018

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRDKWIVDGGYTHETALQAARQLLS
CCCCCEEEEECCCEEEEEEECCCCHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLISGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC
>Mature Secondary Structure 
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRDKWIVDGGYTHETALQAARQLLS
CCCCCEEEEECCCEEEEEEECCCCHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLISGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]