Click here to switch to the map view.

The map label for this gene is puuE

Identifier: 218694878

GI number: 218694878

Start: 1507276

End: 1508541

Strand: Direct

Name: puuE

Synonym: EC55989_1464

Alternate gene names: 218694878

Gene position: 1507276-1508541 (Clockwise)

Preceding gene: 218694877

Following gene: 218694880

Centisome position: 29.24

GC content: 57.58

Gene sequence:

>1266_bases
ATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCTACCCCACGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCA
GTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTAAATA
CCGGCCACCGCCACCCTGAACTGGTCGCGGCGGTAGAGCAGCAACTGCAACAGTTTACCCACACCGCGTATCAGATTGTG
CCGTATGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCCCTTGCCCCGGTGAGCGGGCAGGCCAAAACCGCGTTCTT
CACTACCGGCGCGGAAGCGGTGGAAAACGCGGTGAAAATTGCTCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTA
GCGGCGGCTTCCACGGTCGTACGTATATGACTATGGCGTTAACTGGAAAGGTCGCTCCGTACAAAATCGGCTTCGGCCCG
TTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTCGACGCCATCGA
ACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCA
ACGTTGCGCCAAAAGAGCTGGTTGCCGCCATTCGCCGCCTGTGCGACGAGCACGGCATTGTGATGATTGCCGATGAAGTG
CAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGTCGATAAGCCGGATTTAATGACGATGGCGAA
AAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCGCCCGCGCCGGGCGGGTTGG
GTGGCACCTACGCCGGGAACCCGCTGGCGGTGGCTGCCGCGCCCGCGGTGCTCAATATTATCGACAAAGAATCACTCTGC
GAACGCGCGAATCAACTGGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGT
ACGCGGCCTGGGGTCGATGATTGCGGCAGAGTTTAACGATCCGCAAACGGGCAAGCCGTCAGCGGCGATTGCACAGAAAA
TCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCG
CTGACCATCCCGGATGTGCAATTCGATGCGGCAATGAAAATTTTGCAGGATGCGCTGAGCGATTAA

Upstream 100 bases:

>100_bases
CGAGTACCGTTTACCGTGATGGGCGCAGCCTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACAGTGA
AGAGTCAAAAGGTGTGAAAC

Downstream 100 bases:

>100_bases
TAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGC
ATTCACGCCGCATCCTGCAA

Product: 4-aminobutyrate transaminase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIV
PYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
QSGFARTGKLFAMDHYVDKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAPAVLNIIDKESLC
ERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYP
LTIPDVQFDAAMKILQDALSD

Sequences:

>Translated_421_residues
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIV
PYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
QSGFARTGKLFAMDHYVDKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAPAVLNIIDKESLC
ERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYP
LTIPDVQFDAAMKILQDALSD
>Mature_420_residues
SNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIVP
YESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPF
PGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEVQ
SGFARTGKLFAMDHYVDKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAPAVLNIIDKESLCE
RANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPL
TIPDVQFDAAMKILQDALSD

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=400, Percent_Identity=32.5, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI284507298, Length=322, Percent_Identity=32.6086956521739, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI13994255, Length=417, Percent_Identity=30.2158273381295, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI37574042, Length=448, Percent_Identity=28.125, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI188536080, Length=445, Percent_Identity=28.5393258426966, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI38679950, Length=445, Percent_Identity=28.5393258426966, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI38679946, Length=445, Percent_Identity=28.5393258426966, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI24119277, Length=392, Percent_Identity=27.5510204081633, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI226442709, Length=389, Percent_Identity=29.0488431876607, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI226442705, Length=448, Percent_Identity=27.6785714285714, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787560, Length=421, Percent_Identity=98.5748218527316, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=421, Percent_Identity=54.8693586698337, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1789759, Length=404, Percent_Identity=33.1683168316832, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788044, Length=388, Percent_Identity=35.0515463917526, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI145693181, Length=403, Percent_Identity=34.9875930521092, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1786349, Length=348, Percent_Identity=31.6091954022989, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1786991, Length=349, Percent_Identity=29.7994269340974, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25144271, Length=407, Percent_Identity=32.9238329238329, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI32564660, Length=422, Percent_Identity=32.2274881516588, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71992977, Length=410, Percent_Identity=29.7560975609756, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25144274, Length=295, Percent_Identity=32.5423728813559, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17541228, Length=426, Percent_Identity=25.3521126760563, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71981843, Length=155, Percent_Identity=33.5483870967742, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323470, Length=408, Percent_Identity=32.5980392156863, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6324432, Length=406, Percent_Identity=31.2807881773399, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324386, Length=314, Percent_Identity=25.4777070063694, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321456, Length=358, Percent_Identity=26.536312849162, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357415, Length=404, Percent_Identity=31.9306930693069, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI161085790, Length=420, Percent_Identity=30.2380952380952, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28574759, Length=419, Percent_Identity=29.8329355608592, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21356575, Length=430, Percent_Identity=28.6046511627907, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI281366494, Length=364, Percent_Identity=27.1978021978022, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24667139, Length=364, Percent_Identity=27.1978021978022, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24667143, Length=364, Percent_Identity=27.1978021978022, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 44731; Mature: 44599

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPE
CCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCCEEHHHHHHHHCCCCCCCHH
LVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
ARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHGISTQ
HHHCCCCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCEEECCCCCCCCCCCCH
DSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
HHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHH
QSGFARTGKLFAMDHYVDKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGN
HCCHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEECCCCEECCCCCCCCCCCCCCC
PLAVAAAPAVLNIIDKESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAAEFND
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
PQTGKPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDVQFDAAMKILQDALS
CCCCCCHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
D
C
>Mature Secondary Structure 
SNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPE
CCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCCEEHHHHHHHHCCCCCCCHH
LVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
ARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHGISTQ
HHHCCCCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCEEECCCCCCCCCCCCH
DSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
HHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHH
QSGFARTGKLFAMDHYVDKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGN
HCCHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEECCCCEECCCCCCCCCCCCCCC
PLAVAAAPAVLNIIDKESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAAEFND
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
PQTGKPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDVQFDAAMKILQDALS
CCCCCCHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]