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The map label for this gene is rluB

Identifier: 218694839

GI number: 218694839

Start: 1468556

End: 1469455

Strand: Direct

Name: rluB

Synonym: EC55989_1425

Alternate gene names: 218694839

Gene position: 1468556-1469455 (Clockwise)

Preceding gene: 218694838

Following gene: 218694843

Centisome position: 28.49

GC content: 54.33

Gene sequence:

>900_bases
GTGAAGGCGACACTAAAAGGAAGCTCTATGAGCGAAAAATTACAGAAAGTGCTGGCCCGCGCTGGCCACGGCTCCCGCCG
TGAAATCGAATCTATTATTGAAGCCGGTCGTGTGAGTGTTGATGGCAAAATTGCTAAACTCGGCGATCGCGTTGAAGTTA
CCCCTGGGCTGAAAATTCGTATCGATGGTCATCTGATTTCGGTACGTGAGTCCGCTGAACAAATTTGTCGCGTTCTGGCC
TATTACAAACCGGAAGGTGAGTTGTGTACCCGTAACGATCCGGAAGGGCGTCCGACGGTGTTTGACCGTCTGCCAAAGCT
GCGTGGCGCACGCTGGATTGCCGTAGGTCGTCTGGACGTTAACACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAAC
TGGCAAACCGTTTAATGCATCCAAGCCGTGAAGTTGAACGTGAATACGCCGTGCGTGTATTTGGTCAGGTTGACGACGCG
AAACTGCGTGATTTGAGTCGTGGCGTGCAACTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGA
AGGGATCAACCAGTGGTACAACGTGACTCTGACCGAAGGCCGTAACCGTGAAGTTCGTCGTCTGTGGGAAGCGGTTGGTG
TGCAGGTGAGCCGCCTGATCCGTGTTCGTTACGGTGATATCCCACTGCCAAAAGGTCTGCCACGCGGTGGCTGGACGGAG
CTGGACCTCGCCCAGACTAACTATCTGCGCGAACTGGTGGAGCTACCGCCAGAAACCAGCTCTAAAGTCGCTGTAGAAAA
AGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTG
GCGGGCGTAATAACGGTTAA

Upstream 100 bases:

>100_bases
GTAGGTGATGTGAAGCCTTTCTTATAAGTGCGTAACTCTGTATACTACGCGGCCTTAAAATGGCGCTATACGGCGCCATT
GATTCTCCTGTTCGACGCCT

Downstream 100 bases:

>100_bases
TCAAAGTATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCAATCCCTATTTGCGCCTTAACACCGGCATCAA
ACGCATGTTTGATGGGGCGC

Product: 23S rRNA pseudouridylate synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKATLKGSSMSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRESAEQICRVLA
YYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDA
KLRDLSRGVQLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTE
LDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG

Sequences:

>Translated_299_residues
MKATLKGSSMSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRESAEQICRVLA
YYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDA
KLRDLSRGVQLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTE
LDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG
>Mature_299_residues
MKATLKGSSMSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRESAEQICRVLA
YYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDA
KLRDLSRGVQLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTE
LDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=289, Percent_Identity=100, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1790453, Length=293, Percent_Identity=29.6928327645051, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=30.3418803418803, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=33.6787564766839, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 33502; Mature: 33502

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATLKGSSMSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIR
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEECCCEEEE
IDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDV
ECCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEC
NTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQLEDGPAAFKT
CCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEE
IKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTE
EEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
LDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG
EHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKATLKGSSMSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIR
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEECCCEEEE
IDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDV
ECCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEC
NTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQLEDGPAAFKT
CCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEE
IKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTE
EEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
LDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHSQVSGGRRSGGRNNG
EHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]