Click here to switch to the map view.

The map label for this gene is hyaF

Identifier: 218694510

GI number: 218694510

Start: 1145416

End: 1146273

Strand: Direct

Name: hyaF

Synonym: EC55989_1085

Alternate gene names: 218694510

Gene position: 1145416-1146273 (Clockwise)

Preceding gene: 218694509

Following gene: 218694511

Centisome position: 22.22

GC content: 54.66

Gene sequence:

>858_bases
ATGAGCGAAACTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGAT
CACCTGTCAGGTGAATGATGAACCGAGTATGGCGGCCCTGGAACAATGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAA
ACGAGTTACAACATCAAATGAGTCAACGCCAGCCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCCACGCC
GACGATCGTCACTTTATCAATACCCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAG
TGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGG
AAGCTGGCTGTGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCC
ATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAG
CATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGA
TTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACC
TTAAAAGGCCCTTTACTGGAAAGTTATGAAATCTGCCCGATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGA
CTCTGCGCAGCGGCTTAGCGAGGTATGTCAGTGGCTGGCGGAAGCTGCACCGACGTAA

Upstream 100 bases:

>100_bases
CCGGCGGAAACTATCGCGGTGTGCTGAATGGTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAA
CCGCAGCAGGAGCGTGCCTC

Downstream 100 bases:

>100_bases
AAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGA
GGGAGATCTACCATTATCGG

Product: protein involved in nickel incorporation into hydrogenase-1 proteins

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHA
DDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT

Sequences:

>Translated_285_residues
MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHA
DDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT
>Mature_284_residues
SETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHAD
DRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPI
DGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTL
KGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT

Specific function: Not known. Could enhance the incorporation of nickel to the hydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupH/hyaF family [H]

Homologues:

Organism=Escherichia coli, GI1787211, Length=285, Percent_Identity=98.9473684210526, Blast_Score=574, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006894 [H]

Pfam domain/function: PF04809 HupH_C [H]

EC number: NA

Molecular weight: Translated: 31471; Mature: 31340

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQM
CCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SQRQPPLGEVLAVDLLNLHADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHEEEEEECHH
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT
HCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQM
CCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SQRQPPLGEVLAVDLLNLHADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHEEEEEECHH
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT
HCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]