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The map label for this gene is hyaD

Identifier: 218694508

GI number: 218694508

Start: 1144437

End: 1145024

Strand: Direct

Name: hyaD

Synonym: EC55989_1083

Alternate gene names: 218694508

Gene position: 1144437-1145024 (Clockwise)

Preceding gene: 218694507

Following gene: 218694509

Centisome position: 22.2

GC content: 57.99

Gene sequence:

>588_bases
ATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGA
ACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGT
ATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCC
GGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGCTGGCGCTGGC
AGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCTATGCTCGACGACTACGGCGGCAGCC
TGAGCGAACTGGCACGGGATCAACTGCCCGCTGCGGAACTATCGGCGTTGGCGCAACTGGCTGCGTGGGGAATTGTGCCG
CAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGTTTGCGCCAGTA
CAGGATGACACTGGAGGAGCAGGGATGA

Upstream 100 bases:

>100_bases
CGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAA
ATAAGTAACAAGGAGCGTTC

Downstream 100 bases:

>100_bases
GCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCGGTCAGTGAATCCCGTCTTGAC
GACTGGCTTACGCAAGCGCC

Product: hydrogenase 1 maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAELSALAQLAAWGIVP
QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG

Sequences:

>Translated_195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAELSALAQLAAWGIVP
QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG
>Mature_194_residues
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAG
ERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAELSALAQLAAWGIVPQ
PANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG

Specific function: Protease involved in the C-terminal processing of hyaB, the large subunit of hydrogenase 1 (Potential) [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=195, Percent_Identity=97.9487179487179, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1789367, Length=162, Percent_Identity=38.2716049382716, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 21519; Mature: 21388

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASH
CCCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELARDQLPAAELSALAQLAAWGIVPQPANESRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCC
GVRLRQYRMTLEEQG
CCEEEEEEEHHHCCC
>Mature Secondary Structure 
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASH
CCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELARDQLPAAELSALAQLAAWGIVPQPANESRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCC
GVRLRQYRMTLEEQG
CCEEEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]