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The map label for this gene is potI

Identifier: 218694331

GI number: 218694331

Start: 958459

End: 959304

Strand: Direct

Name: potI

Synonym: EC55989_0902

Alternate gene names: 218694331

Gene position: 958459-959304 (Clockwise)

Preceding gene: 218694330

Following gene: 218694332

Centisome position: 18.59

GC content: 54.02

Gene sequence:

>846_bases
ATGAATAATTTACCGGTAGTTCGTTCACCCTGGCGGATTGTGATTTTGCTGCTGGGCTTTACCTTTCTCTACGCGCCAAT
GCTGATGCTGGTTATCTATTCGTTTAACAGCTCGAAGCTGGTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGTG
AGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGCCTGACAATTGCTGCCTGTGCGGCAACGGCGGCGGCG
ATCCTCGGGACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGATTTCGCGGATCAAATGGCTTTGCCTTTATGATCAC
CGCGCCGCTGGTGATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCT
GGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCT
TCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGT
CATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGCTGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGA
TCGCCAGCTTTGTTTCCGGGCCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCCG
GAAATCAACGCCCTTGCAACGTTAATTCTCGGTGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGC
AGAAAAACAGCGGATACGCGATATCCAGCGTGCAAGACATGGCTGA

Upstream 100 bases:

>100_bases
TGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCA
AAAAGCGTGGGAGAACACGG

Downstream 100 bases:

>100_bases
AGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGCCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGA
TTTTTTAAGAAAACATCTTC

Product: putrescine transporter subunit: membrane component of ABC superfamily

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAA
ILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARHG

Sequences:

>Translated_281_residues
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAA
ILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARHG
>Mature_281_residues
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAA
ILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARHG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=281, Percent_Identity=99.644128113879, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=40.5857740585774, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=31.25, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=27.3584905660377, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30522; Mature: 30522

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
MMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
LLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARHG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
MMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
LLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQRARHG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]