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The map label for this gene is frmB

Identifier: 218693820

GI number: 218693820

Start: 404593

End: 405426

Strand: Reverse

Name: frmB

Synonym: EC55989_0364

Alternate gene names: 218693820

Gene position: 405426-404593 (Counterclockwise)

Preceding gene: 218693821

Following gene: 218693810

Centisome position: 7.86

GC content: 49.04

Gene sequence:

>834_bases
ATGGAACTCATTGAAAAACATGCCAGCTTTGGCGGCTGGCAAAATGTGTATCGGCATTATTCCCAATCACTGAAATGTGA
AATGAATGTCGGCGTCTATCTCCCACCAAAAGCCGCGAATGAAAAATTGCCGGTGCTGTACTGGCTTTCGGGCCTGACCT
GCAACGAGCAGAATTTCATTACTAAATCGGGGATGCAGCGTTACGCGGCTGAACACAACATTATTGTTGTTGCGCCGGAC
ACCAGTCCGCGAGGCAGTCATGTCGCAGATGCCGACCGTTACGATCTCGGGCAAGGTGCCGGGTTTTACCTGAACGCAAC
GCAAGCGCCGTGGAATGAACATTACAAAATGTATGACTATATCCGCAACGAGCTGCCGGATTTAGTGATGCAACATTTTC
CGGCAACGACCAGAAAGTCTATCTCTGGTCATTCTATGGGCGGGCTGGGCGCGCTGGTGCTGGCGTTACGTAACCCAGAT
GAATATGTCAGCGTCTCGGCGTTTTCGCCCATTGTCTCCCCATCGCAAGTACCGTGGGGACAGCAAGCCTTTGCCGCATA
TCTTGGTGAAAATAAAGATGCCTGGTTGGATTACGATCCGGTGAGTCTTATTTCACAAGGTCAACGCGTTGCGGAAATCA
TGGTTGATCAGGGGTTGAGTGATGATTTTTACGCGGAACAGCTGCGGACTCCAAATCTTGAAAAGATCTGCCAGGAGATG
AATATCAAGACGTTAATCCGTTATCACGAGGGTTACGACCACAGCTATTATTTTGTCTCCAGTTTTATTGGCGAGCATAT
TGCCTACCACGCCAATAAACTGAATATGCGTTGA

Upstream 100 bases:

>100_bases
GTTACTGATTTCCCGCAGGTATACCCCGTCCACTTCAGACGGGGTTCTTAATACTCTCCCTGGGCAGCCGTCCGGGGGAT
TAACCGTGAGATAATGACTG

Downstream 100 bases:

>100_bases
TAATAGTGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACACTTCGCCCGTAAACCGTAGGCCTGATAAGA
CGCGCAAAGCGTCGCATCAG

Product: S-formylglutathione hydrolase

Products: NA

Alternate protein names: FGH

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPD
TSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR

Sequences:

>Translated_277_residues
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPD
TSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
>Mature_277_residues
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPD
TSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family

Homologues:

Organism=Homo sapiens, GI33413400, Length=280, Percent_Identity=49.6428571428571, Blast_Score=289, Evalue=2e-78,
Organism=Escherichia coli, GI1786551, Length=277, Percent_Identity=97.8339350180505, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1788477, Length=276, Percent_Identity=54.3478260869565, Blast_Score=315, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17510185, Length=280, Percent_Identity=46.0714285714286, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6322393, Length=298, Percent_Identity=41.2751677852349, Blast_Score=216, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45551932, Length=281, Percent_Identity=50.5338078291815, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24648347, Length=281, Percent_Identity=50.5338078291815, Blast_Score=285, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SFGH1_ECO24 (A7ZIA3)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461532.1
- ProteinModelPortal:   A7ZIA3
- SMR:   A7ZIA3
- STRING:   A7ZIA3
- EnsemblBacteria:   EBESCT00000020302
- GeneID:   5587379
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0380
- eggNOG:   COG0627
- GeneTree:   EBGT00050000011328
- HOGENOM:   HBG286713
- OMA:   KRSIMGH
- ProtClustDB:   CLSK879655
- BioCyc:   ECOL331111:ECE24377A_0380-MONOMER
- InterPro:   IPR000801
- InterPro:   IPR014186
- PANTHER:   PTHR10061
- TIGRFAMs:   TIGR02821

Pfam domain/function: PF00756 Esterase

EC number: =3.1.2.12

Molecular weight: Translated: 31400; Mature: 31400

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: ACT_SITE 145-145 ACT_SITE 221-221 ACT_SITE 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFI
CCHHHHHHCCCCHHHHHHHHHHCCEEEECCCEEECCCCCCCCCCEEEEECCCEECCCCCH
TKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDY
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
IRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPDEYVSVSAFSPIVSPSQVPWG
HHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHEEEEECCCCCEEEEECCCCCCCCCCCCCC
QQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
HHHHHHHHCCCCCCEECCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHC
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFI
CCHHHHHHCCCCHHHHHHHHHHCCEEEECCCEEECCCCCCCCCCEEEEECCCEECCCCCH
TKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDY
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
IRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPDEYVSVSAFSPIVSPSQVPWG
HHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHEEEEECCCCCEEEEECCCCCCCCCCCCCC
QQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
HHHHHHHHCCCCCCEECCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHC
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA