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The map label for this gene is yafC

Identifier: 218693670

GI number: 218693670

Start: 231938

End: 232852

Strand: Reverse

Name: yafC

Synonym: EC55989_0207

Alternate gene names: 218693670

Gene position: 232852-231938 (Counterclockwise)

Preceding gene: 218693673

Following gene: 218693665

Centisome position: 4.52

GC content: 49.4

Gene sequence:

>915_bases
ATGAAAGCCACGTCGGAAGAACTCGCCATTTTTGTTTCGGTCGTCGAAAGCGGCAGCTTTAGCCGGGCAGCGGAACAATT
AGGGCAAGCAAACTCAGCGGTAAGCCGGGCGGTGAAAAAGCTGGAGATGAAACTTGGCGTTAGCCTGCTTAATCGGACCA
CGCGACAACTTAGCCTGACGGAAGAAGGCGAGCGTTATTTTCGTCGCGTACAGTCAATTTTGCAGGAGATGGCATCGGCA
GAATCAGAAATTATGGAGACGCGTAATACACCGCGTGGACTGTTACGGATCGATGCCGCAACTCCAGTGGTGCTGCACTT
TCTGATGCCGTTAATTAAGCCTTTCCGTGAACGCTATCCGGAAGTCACTTTGTCGCTAGTCTCCTCCGAAACGATTATTA
ATTTGATCGAAAGAAAAGTGGATGTCGCGATACGCGCTGGTACGTTAACGGATTCCAGCTTACGTGCCAGGCCGTTATTT
AACAGTTATCGAAAAATTATCGCCTCCCCCGATTATATTTCCCGCTACGGGAAGCCAGAAACGATCGACGATTTAAAGCA
ACATATTTGCCTGGGATTCACTGAACCCGCTTCCCTCAATACCTGGCCGATAGCCCGTAGCGATGGACAATTACATGAGG
TGAAGTACGGTTTGTCATCCAATAGTGGGGAAACACTGAAACAGCTTTGCCTGAGTGGCAACGGGATTGCGTGTTTGTCC
GACTACATGATCGACAAAGAAATCGCTCGCGGAGAATTGGTGGAGTTAATGGCAGATAAAGTGTTGCCAGTGGAAATGCC
ATTCAGTGCAGTCTATTACAGCGACCGTGCGGTAAGTACGCGCATCCGGGCTTTTATCGATTTCCTTAGCGAGCATGTAA
AAACAGCTCCCGGAGGAGCTGTCAGAGAGGCTTAA

Upstream 100 bases:

>100_bases
CATTATGGAGAACATTAAATCGCATGACATATCACGCATCCTGATGGTTTAATGACAAATAAATTCAAAATATCTCTTCT
ATTTTGCAACAGGAGCAAAA

Downstream 100 bases:

>100_bases
TCCTGAGAGATCCCCTCATAATTTCCCCAAAACGTAACCATGTGTGAATAGATTTTGAGTAAGCAGGGTTGCAGCCACGA
GTGAGTCTTCCCTTGTTATT

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA

Sequences:

>Translated_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA
>Mature_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=99.6710526315789, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=33.5640138408304, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=28.7162162162162, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.3515358361775, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=31.5972222222222, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=28.1879194630872, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=29.1666666666667, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789173, Length=284, Percent_Identity=28.8732394366197, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=26.1744966442953, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=171, Percent_Identity=32.1637426900585, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788481, Length=303, Percent_Identity=25.7425742574257, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082132, Length=313, Percent_Identity=26.8370607028754, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=26.7716535433071, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=165, Percent_Identity=28.4848484848485, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788748, Length=126, Percent_Identity=34.9206349206349, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786508, Length=263, Percent_Identity=25.8555133079848, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=245, Percent_Identity=24.4897959183673, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789506, Length=281, Percent_Identity=25.9786476868327, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787806, Length=169, Percent_Identity=25.4437869822485, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786713, Length=122, Percent_Identity=31.9672131147541, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33792; Mature: 33792

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISRYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC
>Mature Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISRYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]