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The map label for this gene is hemL

Identifier: 218693620

GI number: 218693620

Start: 173580

End: 174860

Strand: Reverse

Name: hemL

Synonym: EC55989_0148

Alternate gene names: 218693620

Gene position: 174860-173580 (Counterclockwise)

Preceding gene: 218693623

Following gene: 218693613

Centisome position: 3.39

GC content: 55.58

Gene sequence:

>1281_bases
ATGAGTAAGTCTGAAAATCTTTACAGCGCAGCGCGCGAGCTGATCCCTGGCGGTGTGAACTCCCCTGTTCGCGCCTTTAC
TGGCGTGGGCGGCACTCCACTGTTTATCGAAAAAGCGGACGGCGCCTATCTGTACGATGTTGATGGCAAAGCCTATATCG
ATTATGTCGGTTCCTGGGGGCCGATGGTGCTGGGCCATAACCATCCGGCGATCCGCAATGCCGTGATTGAAGCCGCCGAG
CGTGGTTTAAGCTTTGGTGCACCAACCGAAATGGAAGTGAAAATGGCGCAACTGGTGACCGAACTGGTCCCGACCATGGA
TATGGTGCGCATGGTGAACTCCGGCACTGAAGCGACCATGAGCGCCATCCGCCTGGCCCGTGGTTTTACCGGTCGCGACA
AAATCATTAAATTTGAAGGTTGTTACCACGGTCACGCTGACTGCCTGCTGGTGAAAGCCGGTTCTGGCGCACTCACGTTA
GGCCAGCCAAACTCGCCGGGTGTTCCGGCAGATTTCGCCAAACATACCTTAACCTGTACTTATAACGATCTGGCTTCTGT
ACGCGCCGCGTTTGAGCAATACCCGCAAGAGATTGCCTGTATTATCGTCGAGCCGGTGGCAGGCAATATGAACTGCGTTC
CACCGCTGCCAGAGTTCCTGCCAGGTCTGCGCGCGCTGTGCGACGAATTTGGCGCATTGCTGATCATCGATGAAGTAATG
ACTGGCTTCCGCGTAGCGCTAGCTGGCGCACAGGATTATTACGGTGTGGAACCGGATCTCACCTGCCTGGGCAAAATCAT
CGGCGGTGGAATGCCGGTAGGCGCATTCGGTGGTCGTCGTGATGTAATGGATGCGCTGGCCCCGACGGGTCCGGTCTATC
AGGCGGGTACGCTTTCCGGTAACCCAATTGCGATGGCAGCGGGTTTCGCCTGTCTGAATGAAGTCGCGCAACCGGGCGTT
CACGAAACGTTGGATGAGCTGACATCACGTCTGGCAGAAGGTCTGCTGGAAGCGGCAGAAGAAGCCGGAATTCCGCTGGT
CGTTAACCACGTTGGCGGCATGTTCGGTATTTTCTTTACCGACGCCGAGTCCGTGACGTGCTATCAGGATGTGATGGCCT
GTGACGTGGAACGCTTTAAGCGTTTCTTCCATATGATGCTGGATGAAGGTGTTTACCTGGCACCGTCAGCGTTTGAAGCG
GGCTTTATGTCCGTGGCGCACAGCATGGAAGATATCAATAACACCATCGATGCTGCACGTCGGGTGTTTGCGAAGTTGTG
A

Upstream 100 bases:

>100_bases
TGCACCAGTACAAGCAGCCTGATGTTTGACGAGTATTTAACTTGTTATGAATAACATAGAATAGCAGCCATTCACTTTTT
CTACGAATCAGGAACCCTCC

Downstream 100 bases:

>100_bases
TGATTTTGCCTCGCCCACCGGGCGAGGCATTCAATTAACGGCTCTGCTTTCTCAACAAATAGATAAAATATGGCGCGCCG
ATAAAGGTTGACAGCAGCCC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAE
RGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGV
HETLDELTSRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEA
GFMSVAHSMEDINNTIDAARRVFAKL

Sequences:

>Translated_426_residues
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAE
RGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGV
HETLDELTSRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEA
GFMSVAHSMEDINNTIDAARRVFAKL
>Mature_425_residues
SKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAER
GLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLG
QPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVMT
GFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVH
ETLDELTSRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAG
FMSVAHSMEDINNTIDAARRVFAKL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=315, Percent_Identity=29.8412698412698, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI13994255, Length=376, Percent_Identity=26.8617021276596, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI284507298, Length=230, Percent_Identity=30.4347826086957, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=99.2957746478873, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=446, Percent_Identity=30.2690582959641, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1787560, Length=346, Percent_Identity=32.0809248554913, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1788044, Length=312, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789759, Length=317, Percent_Identity=31.8611987381703, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693181, Length=334, Percent_Identity=29.6407185628743, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1786991, Length=294, Percent_Identity=27.2108843537415, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=299, Percent_Identity=29.0969899665552, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=360, Percent_Identity=28.6111111111111, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32564660, Length=330, Percent_Identity=25.1515151515152, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=142, Percent_Identity=34.5070422535211, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=340, Percent_Identity=29.7058823529412, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6324432, Length=323, Percent_Identity=27.5541795665635, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21357415, Length=301, Percent_Identity=27.2425249169435, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356575, Length=321, Percent_Identity=28.0373831775701, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45357; Mature: 45225

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
CCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEECCCCC
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
HHHHHHEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLNEVAQPGVHETLDELTSRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFAKL
HHHHCC
>Mature Secondary Structure 
SKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
CCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEECCCCC
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
HHHHHHEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLNEVAQPGVHETLDELTSRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFAKL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA