| Definition | Escherichia coli 55989, complete genome. |
|---|---|
| Accession | NC_011748 |
| Length | 5,154,862 |
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The map label for this gene is thrA
Identifier: 218693478
GI number: 218693478
Start: 349
End: 2811
Strand: Direct
Name: thrA
Synonym: EC55989_0002
Alternate gene names: 218693478
Gene position: 349-2811 (Clockwise)
Preceding gene: 218693477
Following gene: 218693479
Centisome position: 0.01
GC content: 53.19
Gene sequence:
>2463_bases ATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAA TGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAA CCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCC GCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGG CATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTA TGGCCGGCGTGTTAGAAGCGCGTGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTAC CTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGTTGAT GGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGC TGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTG CCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCC CCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGC TCATTGGTGCCAGTCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTT TCCGGCCCGGGGATGAAAGGAATGGTCGGCATGGCGGCGCGCGTCTTTGCCGCGATGTCACGCGCCCGTATTTCCGTGGT GCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAA TGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCG GTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACAT TGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCG TTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTG CTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAA GGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATC TCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACCTCCAGCCAGGCAGTG GCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGA TTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTAC CGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTT TCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGA ACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAAC TGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCG AATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGG CAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATG GCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGAC GTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGA
Upstream 100 bases:
>100_bases CAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCA AAGGTAACGAGGTAACAACC
Downstream 100 bases:
>100_bases CATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTG TTGATGGTGCATTGCTCGGA
Product: bifunctional aspartokinase I/homeserine dehydrogenase I
Products: NA
Alternate protein names: Aspartokinase I/homoserine dehydrogenase I; AKI-HDI; Aspartokinase; Homoserine dehydrogenase [H]
Number of amino acids: Translated: 820; Mature: 820
Protein sequence:
>820_residues MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDALPNISDAERIFAELLTGLAA AQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSV SGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIIS VVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAV ADQYADFLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSL SYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAGND VTAAGVFADLLRTLSWKLGV
Sequences:
>Translated_820_residues MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDALPNISDAERIFAELLTGLAA AQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSV SGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIIS VVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAV ADQYADFLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSL SYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAGND VTAAGVFADLLRTLSWKLGV >Mature_820_residues MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDALPNISDAERIFAELLTGLAA AQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSV SGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIIS VVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAV ADQYADFLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSL SYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAGND VTAAGVFADLLRTLSWKLGV
Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]
COG id: COG0527
COG function: function code E; Aspartokinases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 2 ACT domains [H]
Homologues:
Organism=Escherichia coli, GI1786183, Length=820, Percent_Identity=99.6341463414634, Blast_Score=1679, Evalue=0.0, Organism=Escherichia coli, GI1790376, Length=821, Percent_Identity=30.5724725943971, Blast_Score=345, Evalue=7e-96, Organism=Escherichia coli, GI1790455, Length=471, Percent_Identity=30.1486199575372, Blast_Score=162, Evalue=1e-40, Organism=Saccharomyces cerevisiae, GI6322599, Length=340, Percent_Identity=33.8235294117647, Blast_Score=168, Evalue=3e-42, Organism=Saccharomyces cerevisiae, GI6320893, Length=531, Percent_Identity=26.7419962335217, Blast_Score=166, Evalue=1e-41,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002912 - InterPro: IPR001048 - InterPro: IPR005106 - InterPro: IPR001341 - InterPro: IPR018042 - InterPro: IPR011147 - InterPro: IPR001342 - InterPro: IPR019811 - InterPro: IPR016040 [H]
Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]
EC number: =2.7.2.4; =1.1.1.3 [H]
Molecular weight: Translated: 89052; Mature: 89052
Theoretical pI: Translated: 5.47; Mature: 5.47
Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 4.3 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCCCC LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP EEEECCCCCEEHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV CCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD CHHHHHHHCCHHHHHHHHHHCHHHCCCCCCCCHHHEECCEEEECCCCCCCCCEEEECCCC EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF CCCCCCHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCEEEEE CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL HHCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCHHHEEEEEEEECCCCCHHH LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL HHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHH VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSR HHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA CCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHH REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHH NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAF HHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCEEEEECCCCHHHH YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV HHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCCCC LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP EEEECCCCCEEHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV CCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD CHHHHHHHCCHHHHHHHHHHCHHHCCCCCCCCHHHEECCEEEECCCCCCCCCEEEECCCC EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF CCCCCCHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCEEEEEEEEECCCCEEEEE CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL HHCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCHHHEEEEEEEECCCCCHHH LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL HHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHH VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSR HHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA CCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHH REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA HHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHH NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAF HHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCEEEEECCCCHHHH YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV HHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7003595; 1630901; 7610040; 9278503; 6277952; 2410621; 387092; 6298218; 390305 [H]