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The map label for this gene is rpiR

Identifier: 218692386

GI number: 218692386

Start: 4789498

End: 4790388

Strand: Reverse

Name: rpiR

Synonym: ECED1_4823

Alternate gene names: 218692386

Gene position: 4790388-4789498 (Counterclockwise)

Preceding gene: 218692389

Following gene: 218692385

Centisome position: 91.95

GC content: 47.81

Gene sequence:

>891_bases
ATGAGCCAGTCAGAGTTTGATTCAGCGCTTCCGAACGGTATCGGGTTAGCGCCTTACCTGCGAATGAAGCAGGAAGGAAT
GACAGAAAACGAAAGCCGCATCGTGGAGTGGTTACTCAAACCCGGTAACCTGAGTTGTGCACCCGCAATTAAAGATGTCG
CAGAAGCTCTGGCGGTATCTGAAGCGATGATCGTTAAGGTATCAAAGCTGCTGGGGTTTAGCGGCTTTCGTAACTTACGC
AGTGCGCTGGAAGATTATTTTTCTCAGTCAGAACAAGTATTGCCTTCCGAGTTGGCTTTTGATGAAGCGCCGCAGGATGT
GGTGAATAAGGTATTTAACATCACTTTACGCACCATTATGGAAGGTCAGTCGATCGTCAACGTTGATGAGATCCACCGTG
CCGCCCGCTTTTTCTATCAGGCCAGACAGCGGGATTTGTACGGTGCCGGAGGATCAAATGCTATCTGTGCTGATGTACAG
CACAAGTTTTTGCGCATTGGCGTACGCTGCCAGGCCTATCCGGACGCTCACATCATGATGATGTCTGCTTCGTTGTTACA
GGAAGGAGATGTGGTGCTGGTGGTGACTCATTCCGGGCGAACCAGTGATGTAAAAGCGGCTGTAGAACTGGCAAAAAAGA
ACGGGGCAAAGATTATTTGTATAACGCATAGCTATCATTCACCAATAGCGAAGCTGGCCGATTATATTATTTGCTCACCG
GCCCCGGAAACACCGTTATTAGGTCGTAATGCCTCGGCAAGAATATTACAACTGACTTTGCTGGACGCGTTTTTTGTCTC
TGTCGCCCAGCTCAATATTGAACAAGCTAATATTAATATGCAAAAAACCGGCGCAATTGTTGATTTCTTCTCACCAGGCG
CGCTGAAATAA

Upstream 100 bases:

>100_bases
AAACTTTATAAATTTTATTTTTTTGTGAAGTTGCCAGCATCTTTTCTGTTCTTGCTGTGGTGATATAGTGGCGTCTTCAA
TTCAAGGACAAGAGAACGTG

Downstream 100 bases:

>100_bases
GAAAAAGGTAATTCCGCCATCGCGGAGTTACCTCTCCCCTTACCGTGAGAACATGATTATGAATAAATATCTGAAATATT
TCAGCGGCACACTCGTGGGC

Product: DNA-binding transcriptional repressor RpiR

Products: NA

Alternate protein names: Als operon repressor

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLR
SALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSP
APETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANINMQKTGAIVDFFSPGALK

Sequences:

>Translated_296_residues
MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLR
SALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSP
APETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANINMQKTGAIVDFFSPGALK
>Mature_295_residues
SQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRS
ALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQH
KFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSPA
PETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANINMQKTGAIVDFFSPGALK

Specific function: Regulatory protein involved in rpiB gene repression. Also involved in als operon repression

COG id: COG1737

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain

Homologues:

Organism=Escherichia coli, GI87082369, Length=296, Percent_Identity=100, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1788767, Length=254, Percent_Identity=27.1653543307087, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1788159, Length=240, Percent_Identity=24.5833333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082130, Length=274, Percent_Identity=21.5328467153285, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPIR_ECOL6 (P0ACS8)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_756946.2
- ProteinModelPortal:   P0ACS8
- SMR:   P0ACS8
- EnsemblBacteria:   EBESCT00000042410
- GeneID:   1038300
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c5095
- GeneTree:   EBGT00050000010596
- HOGENOM:   HBG725281
- OMA:   NENSIIE
- ProtClustDB:   PRK11337
- InterPro:   IPR000281
- InterPro:   IPR001347
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF01418 HTH_6; PF01380 SIS

EC number: NA

Molecular weight: Translated: 32363; Mature: 32231

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS51071 HTH_RPIR; PS51464 SIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSCAPAIKDVAEALAVS
CCCHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
EAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIM
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHCCCHHHCCCCCCHHHHHHHHHHHHHHHH
EGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQHKFLRIGVRCQAYPDAHIMM
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHH
MSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSP
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHEEECC
APETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANINMQKTGAIVDFFSPGALK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEECCCCCCC
>Mature Secondary Structure 
SQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSCAPAIKDVAEALAVS
CCHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
EAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDEAPQDVVNKVFNITLRTIM
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHCCCHHHCCCCCCHHHHHHHHHHHHHHHH
EGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQHKFLRIGVRCQAYPDAHIMM
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCHHHHH
MSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSP
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHEEECC
APETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANINMQKTGAIVDFFSPGALK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157