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The map label for this gene is rfaJ [H]

Identifier: 218691911

GI number: 218691911

Start: 4236501

End: 4237514

Strand: Reverse

Name: rfaJ [H]

Synonym: ECED1_4310

Alternate gene names: 218691911

Gene position: 4237514-4236501 (Counterclockwise)

Preceding gene: 218691912

Following gene: 218691910

Centisome position: 81.34

GC content: 30.77

Gene sequence:

>1014_bases
TTGGATTTTAAACATCTTACTCAATTTAAAGATATAATTGAACTGGACAAGCGCCCCGTTAAACTTGATGAACGGGAAAC
GTTTAATGTCTCATGGGGTATTGATGAGAATTACCAAGTTGGGGCTGCGATTTCAATTGCTTCAATTCTTGAAAATAATA
AGCAAAACAAATTTACCTTTCACATAATCGCTGATTACCTAGACAAAGAGTATATTGAATTATTATCACAATTAGCAACG
AAGTATCAAACAGTAATTAAATTATATCATATTGATTCTGAGCCATTGAAGGAGCTACCTCAATCAAATATCTGGCCAGT
ATCTATTTATTATCGTTTGCTTTCATTTGATTATTTTTCTGCGCGATTGGATTCATTATTATATCTTGATGCTGATATCG
TCTGTAAGGGGTCATTGAACGAGTTAATAGCATTAGAGTTTAAAGATGAATATGGGGCCGTGGTAATTGATGTAGATGCC
ATGCAAAGTAAAAGTGCTGAGCGTTTGTGTAATGAGGATTTTAACGGTATCTATTTTAACTCTGGTGTAATGTATATTAA
TTTACGGGAATGGTTAAAACAAAGGCTAACGGAGAAATTCTTTGATCTATTATCAGATGAATCAATTATAAAAAAATTAA
AGTATCCGGATCAAGATATTTTAAACTTGATGTTTCTACACCATGCAAAAATATTACCGAGAAAATATAATTGTATTTAT
ACAATAAAGTCAGAATTTGAAGAAAAAAATAGTGAATATTACACCCGGTTTATTAATGATGAAACTGTCTTCATACATTA
TACTGGTGTAACTAAGCCATGGCATGATTGGGCGAACTACGCATCTGCAGATTATTTTCGTAATATTTATAATATATCGC
CATGGAGAAATATACCTTATAAAAAAGCTGTTAAAAAACATGAGTACAAAGAAAAATATAAACACTTGCTTTACCAGAAA
AAATTTCTTGATGGTGTTTTTACAGCAATTAAATATAATGTTATGAAAGGTTAA

Upstream 100 bases:

>100_bases
ATATCTTATATTCAAAAAGAAAATTAAAAAAGCAATCAGGGATGTAAAAGTCAAACTAGGATTGAAGAGCAAATAATAAT
TATGATGAAGGGTAATTAAA

Downstream 100 bases:

>100_bases
TATTCGAGATGGTTGATAAAATAATATTTACAGTTACTCCTATTTTTTCAATTCCTCCTCGCGGAGCGGCCGCTGTTGAA
ACGTGGATGTACCAGGTTGC

Product: lipopolysaccharide 1,2-glucosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAISIASILENNKQNKFTFHIIADYLDKEYIELLSQLAT
KYQTVIKLYHIDSEPLKELPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
MQSKSAERLCNEDFNGIYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQDILNLMFLHHAKILPRKYNCIY
TIKSEFEEKNSEYYTRFINDETVFIHYTGVTKPWHDWANYASADYFRNIYNISPWRNIPYKKAVKKHEYKEKYKHLLYQK
KFLDGVFTAIKYNVMKG

Sequences:

>Translated_337_residues
MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAISIASILENNKQNKFTFHIIADYLDKEYIELLSQLAT
KYQTVIKLYHIDSEPLKELPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
MQSKSAERLCNEDFNGIYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQDILNLMFLHHAKILPRKYNCIY
TIKSEFEEKNSEYYTRFINDETVFIHYTGVTKPWHDWANYASADYFRNIYNISPWRNIPYKKAVKKHEYKEKYKHLLYQK
KFLDGVFTAIKYNVMKG
>Mature_337_residues
MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAISIASILENNKQNKFTFHIIADYLDKEYIELLSQLAT
KYQTVIKLYHIDSEPLKELPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
MQSKSAERLCNEDFNGIYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQDILNLMFLHHAKILPRKYNCIY
TIKSEFEEKNSEYYTRFINDETVFIHYTGVTKPWHDWANYASADYFRNIYNISPWRNIPYKKAVKKHEYKEKYKHLLYQK
KFLDGVFTAIKYNVMKG

Specific function: Adds the glucose(II) group on the galactose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790056, Length=326, Percent_Identity=40.1840490797546, Blast_Score=257, Evalue=9e-70,
Organism=Escherichia coli, GI1790057, Length=305, Percent_Identity=34.4262295081967, Blast_Score=201, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.58 [H]

Molecular weight: Translated: 40154; Mature: 40154

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAISIASILENNKQNKFTF
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEE
HIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQSNIWPVSIYYRLLSFDYFS
EHHHHHHCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
ARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGIYFN
HHHHHHEEECCCEEECCCCCCEEEEEEECCCCEEEEEEHHHHCHHHHHHHCCCCCEEEEE
SGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQDILNLMFLHHAKILPRKYNCIY
CCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
TIKSEFEEKNSEYYTRFINDETVFIHYTGVTKPWHDWANYASADYFRNIYNISPWRNIPY
EEHHHHHHCCCHHEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
KKAVKKHEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAISIASILENNKQNKFTF
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEE
HIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQSNIWPVSIYYRLLSFDYFS
EHHHHHHCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHH
ARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGIYFN
HHHHHHEEECCCEEECCCCCCEEEEEEECCCCEEEEEEHHHHCHHHHHHHCCCCCEEEEE
SGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQDILNLMFLHHAKILPRKYNCIY
CCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
TIKSEFEEKNSEYYTRFINDETVFIHYTGVTKPWHDWANYASADYFRNIYNISPWRNIPY
EEHHHHHHCCCHHEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
KKAVKKHEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]