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The map label for this gene is yiaI [H]

Identifier: 218691862

GI number: 218691862

Start: 4182245

End: 4182718

Strand: Reverse

Name: yiaI [H]

Synonym: ECED1_4261

Alternate gene names: 218691862

Gene position: 4182718-4182245 (Counterclockwise)

Preceding gene: 218691863

Following gene: 218691859

Centisome position: 80.29

GC content: 53.16

Gene sequence:

>474_bases
ATGAACCGGTTTATTATTGCGGATGCGACGAAATGTATCGGTTGCCGTACCTGTGAAGTGGCTTGCGCAGTGTCGCATCA
GGAGAATCAGGATTGCGCTGCGTTGTCACCAGACGAGTTTATTTCCCGTATTCGTGTCATTAAAGATCATACTTACACCA
CGGCAGTAGCCTGTCATCAGTGTGAAGATGCACCGTGCGCAAATGTGTGCCCTGTTGACGCGATAAGCCGCGAACATGGG
CATATTTTCGTTGAACAATCACGTTGCATTGGCTGTAAAAGCTGTATGCTGGCTTGCCCGTTTGGTGCGATGGAGGTCGT
TTCTTCGCGCAAAAAGGCGAGGGCGATTAAGTGCGATCTGTGCTGGCATCGGGCGACGGGGCCGGCCTGTGTTGAAGCCT
GCCCGACAAAGGCGTTGCAGTGCATGGATGTCGAGAAAGTGCAGCGGCATCGGCTACGGCAGCAGCCTGTTTGA

Upstream 100 bases:

>100_bases
CCTTTGGACAAGTGCCAAAACTTTAACATTTCCTTCGTTGGATCAAAGCAGTAGGGACGCGCTCTCTGGCACTCTGCTGT
TTTAGTGCAAAGGAGTGATC

Downstream 100 bases:

>100_bases
AACGTTTTTCCGGCGTTTCTCAACGCAGGGATAGTGCGCTTTGTTTATGCCGGATGCGGCTAAAACGCCTTATCCGGCCT
ACAAATTCTACCAAATTCAA

Product: putative hydrogenase, 4Fe-4S ferredoxin-type component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHTYTTAVACHQCEDAPCANVCPVDAISREHG
HIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV

Sequences:

>Translated_157_residues
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHTYTTAVACHQCEDAPCANVCPVDAISREHG
HIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV
>Mature_157_residues
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHTYTTAVACHQCEDAPCANVCPVDAISREHG
HIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=96.8152866242038, Blast_Score=309, Evalue=7e-86,
Organism=Escherichia coli, GI1789067, Length=162, Percent_Identity=61.7283950617284, Blast_Score=196, Evalue=6e-52,
Organism=Escherichia coli, GI1788811, Length=147, Percent_Identity=51.7006802721088, Blast_Score=144, Evalue=2e-36,
Organism=Escherichia coli, GI87082179, Length=153, Percent_Identity=45.0980392156863, Blast_Score=137, Evalue=2e-34,
Organism=Escherichia coli, GI87082180, Length=156, Percent_Identity=44.8717948717949, Blast_Score=136, Evalue=7e-34,
Organism=Escherichia coli, GI1789079, Length=189, Percent_Identity=38.6243386243386, Blast_Score=127, Evalue=3e-31,
Organism=Escherichia coli, GI87082114, Length=190, Percent_Identity=34.7368421052632, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=33.9393939393939, Blast_Score=90, Evalue=6e-20,
Organism=Escherichia coli, GI1787122, Length=163, Percent_Identity=31.9018404907975, Blast_Score=90, Evalue=7e-20,
Organism=Escherichia coli, GI1787963, Length=161, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI226510944, Length=144, Percent_Identity=36.8055555555556, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI2367345, Length=171, Percent_Identity=32.1637426900585, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17353; Mature: 17353

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
14.0 %Cys+Met (Translated Protein)
11.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
14.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHTYTTAVACHQ
CCEEEEECCCHHHCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHC
CEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDL
CCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHEEEEEE
CWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHTYTTAVACHQ
CCEEEEECCCHHHCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHC
CEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDL
CCCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHEEEEEE
CWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]