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The map label for this gene is gspE [H]

Identifier: 218691611

GI number: 218691611

Start: 3899726

End: 3901207

Strand: Direct

Name: gspE [H]

Synonym: ECED1_3991

Alternate gene names: 218691611

Gene position: 3899726-3901207 (Clockwise)

Preceding gene: 218691610

Following gene: 218691612

Centisome position: 74.86

GC content: 52.16

Gene sequence:

>1482_bases
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAAGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGACTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCCAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATCAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCATCCATCTATGGTGAACGCGCTGTGCTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTAACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATTCTTTCGGCGCTTAA
TACCCCTGGCCGTAATATTCTGACGGTAGAAGATCCCGTGGAATATGAACTGGAAGGCATAGGACAAACGCAGGTGAATA
CCCGCGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAGATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACAGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTCACCCGGCTACGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCGCCCCAGCAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACGACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTACCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Upstream 100 bases:

>100_bases
GAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGAATGAGAACACGTTCAACAGTCACGCCCCTGCGCCAT
CGTCACGGTGAGGCATTCTT

Downstream 100 bases:

>100_bases
ATTATCGCTATCGCGCCATGACCCAGGATGGTCAAAAATTGCAGGGGATCATTGATGCTAACGATGAACGTCAGGCACGA
CTGCGGCTGCGTGAAGAAGG

Product: general secretory pathway component, cryptic

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Sequences:

>Translated_493_residues
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA
>Mature_493_residues
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=493, Percent_Identity=99.3914807302231, Blast_Score=1002, Evalue=0.0,
Organism=Escherichia coli, GI1786296, Length=390, Percent_Identity=43.8461538461538, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI87082188, Length=279, Percent_Identity=36.2007168458781, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54694; Mature: 54694

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEA
CCCCCCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALLISRIKVMARLDIAEKRIPQD
HHHHHCCEEEEECCEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
CCEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHC
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSF
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHH
ARGLRAILRQDPDVVMVGEIRDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGV
HHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCH
ESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFKYHQLAVTTIGTPVGCPHCHQ
HHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCHHC
SGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
CCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VMRVASATLENEA
HHHHHHHHHCCCC
>Mature Secondary Structure
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEA
CCCCCCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALLISRIKVMARLDIAEKRIPQD
HHHHHCCEEEEECCEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
CCEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHC
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSF
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHH
ARGLRAILRQDPDVVMVGEIRDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGV
HHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCH
ESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFKYHQLAVTTIGTPVGCPHCHQ
HHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCHHC
SGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
CCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VMRVASATLENEA
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]