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The map label for this gene is hybC [H]

Identifier: 218691285

GI number: 218691285

Start: 3572625

End: 3574328

Strand: Reverse

Name: hybC [H]

Synonym: ECED1_3644

Alternate gene names: 218691285

Gene position: 3574328-3572625 (Counterclockwise)

Preceding gene: 218691286

Following gene: 218691284

Centisome position: 68.61

GC content: 53.23

Gene sequence:

>1704_bases
ATGAGCCAGAGAATTACTATTGATCCGGTAACCCGTATTGAAGGGCATTTACGCATCGATTGCGAAATCGAAAATGGCGT
CGTTTCGAAAGCATGGGCTTCCGGTACCATGTGGCGCGGCATGGAAGAGATCGTGAAAAACCGCGATCCGCGCGATGCAT
GGATGATTGTGCAACGTATCTGTGGCGTATGTACTACCACTCACGCGCTGTCTTCCGTTCGTGCGGCAGAAAGTGCGCTG
AATATCGACGTTCCGGTTAACGCGCAATACATCCGTAACATCATTCTGGCTGCGCACACCACGCATGACCATATTGTTCA
TTTCTATCAGCTTTCGGCGCTGGACTGGGTGGACATCACTTCTGCACTGCAAGCTGATCCAGCCAAAGCCTCCGAAATGC
TGAAAGGCGTTTCGACCTGGCATCTGAACAGCCCGGAAGAGTTCACCAAAGTTCAGAACAAGATCAAAGATCTGGTTGCC
AGCGGTCAGTTGGGTATTTTCGCCAATGGCTACTGGGGTCACCCGGCAATGAAACTGCCGCCGGAAGTGAACCTGATTGC
GGTAGCGCACTACCTGCAGGCGCTGGAGTGCCAGCGTGACGCTAACCGCGTTGTGGCGCTGCTGGGCGGTAAAACGCCGC
ATATTCAGAACCTGGCGGTAGGTGGTGTCGCGAACCCGATCAACCTCGACGGTCTGGGTGTGCTGAACCTTGAGCGCCTG
ATGTACATCAAGTCCTTCATCGACAAGCTGAGCGACTTTGTTGAGCAGGTTTACAAGGTTGATACTGCAGTTATCGCTGC
GTTCTACCCGGAATGGCTGACGCGCGGTAAAGGTGCGGTGAACTACCTGAGCGTGCCGGAATTCCCGACCGACAGTAAAA
ATGGCAGCTTCCTGTTCCCGGGCGGCTACATTGAGAATGCGGATCTGTCCTCGTATCGTCCGATCACTTCTCATTCCGAT
GAATACCTGATCAAAGGGATTCAGGAAAGCGCGAAGCACTCCTGGTATAAAGACGAAGCGCCGCAGGCACCGTGGGAAGG
TACCACCATTCCGGCTTATGATGGTTGGTCTGACGACGGTAAATATTCCTGGGTGAAATCACCGACTTTCTACGGCAAAA
CGGTAGAAGTGGGTCCGCTGGCTAATATGCTGGTGAAACTGGCGGCAGGACGCGAGTCTACCCAGAACAAACTGAATGAA
ATCGTTGCGATTTATCAGAAACTGACTGGCAACACGCTGGAAGTGGCGCAGCTGCACTCCACGCTGGGCCGTATTATTGG
TCGTACCGTTCACTGCTGTGAATTGCAGGATATCCTGCAAAATCAATACAGTGCACTGATCACCAATATCGGCAAAGGCG
ATCACACCACCTTTGTGAAGCCGAACATTCCGGCAACGGGTGAGTTCAAAGGCGTAGGCTTCCTCGAAGCACCGCGCGGT
ATGCTCTCTCACTGGATGGTGATCAAAGACGGTATTATCAGTAACTACCAGGCGGTTGTTCCATCAACCTGGAACTCTGG
CCCGCGTAACTTCAATGATGACGTCGGTCCTTACGAGCAGTCGCTGGTGGGTACACCGGTTGCCGATCCGAATAAACCGC
TGGAAGTGGTGCGTACCATTCACTCCTTCGACCCGTGCATGGCCTGTGCGGTACACGTAGTGGATGCCGACGGCAACGAA
GTGGTTTCAGTGAAGGTTCTGTAA

Upstream 100 bases:

>100_bases
TGGCTATTGAGATTTGCGCTTATATCGTACTCATTCGTCTACTGCCGATACTTCCTCCTTTAAAACAAAACGATCATAAT
CGTCATGAGGCGAGCAAAGC

Downstream 100 bases:

>100_bases
TGCGTATTTTAGTCTTAGGGGTCGGCAATATTTTGCTGACCGATGAAGCCATCGGTGTGCGGATTGTTGAAGCGTTAGAG
CAACGATACATTCTGCCGGA

Product: hydrogenase 2 large subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESAL
NIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSD
EYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNE
IVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNE
VVSVKVL

Sequences:

>Translated_567_residues
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESAL
NIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSD
EYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNE
IVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNE
VVSVKVL
>Mature_566_residues
SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALN
IDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVAS
GQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERLM
YIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDE
YLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEI
VAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRGM
LSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEV
VSVKVL

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=567, Percent_Identity=99.8236331569665, Blast_Score=1176, Evalue=0.0,
Organism=Escherichia coli, GI1787207, Length=591, Percent_Identity=42.8087986463621, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 62462; Mature: 62331

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CCCCEECCCHHHCCCEEEEEEEECCCEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEECCHHHHEEEHHHCCCCHHHHH
SALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVASGQLGIFANGYWGHPAMKLP
HHHCCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERL
CCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
MYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEECC
GGYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEIVAIYQKLTGNTLEVAQLHS
CEEEECCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHCCHH
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTI
HHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
HSFDPCMACAVHVVDADGNEVVSVKVL
HHCCHHHHHEEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CCCEECCCHHHCCCEEEEEEEECCCEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEECCHHHHEEEHHHCCCCHHHHH
SALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVASGQLGIFANGYWGHPAMKLP
HHHCCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNLAVGGVANPINLDGLGVLNLERL
CCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
MYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRGKGAVNYLSVPEFPTDSKNGSFLFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEECC
GGYIENADLSSYRPITSHSDEYLIKGIQESAKHSWYKDEAPQAPWEGTTIPAYDGWSDDG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTFYGKTVEVGPLANMLVKLAAGRESTQNKLNEIVAIYQKLTGNTLEVAQLHS
CEEEECCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLGRIIGRTVHCCELQDILQNQYSALITNIGKGDHTTFVKPNIPATGEFKGVGFLEAPRG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHCCHH
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPVADPNKPLEVVRTI
HHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
HSFDPCMACAVHVVDADGNEVVSVKVL
HHCCHHHHHEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]