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The map label for this gene is eptA [H]

Identifier: 218691196

GI number: 218691196

Start: 3475015

End: 3475890

Strand: Reverse

Name: eptA [H]

Synonym: ECED1_3549

Alternate gene names: 218691196

Gene position: 3475890-3475015 (Counterclockwise)

Preceding gene: 218691197

Following gene: 218691195

Centisome position: 66.72

GC content: 34.47

Gene sequence:

>876_bases
ATGTTTTTAGTTGTCGGGGAGACAGCAAGAAGTCAAAACTATGCTCTGAATGGATATTCTCGTGGTACCAATGATTTTAC
AAAGAAATATAATGAACTTATATCCTTTCATAATGTGCAGTCGTGTGGCACATCCATAGCCATATCTGTTCCATGCATGT
TTTCAGATATGAAGCGAAAAGAGTTCAATTCCCGTAAAGCGGTAAATTCGGAAAATGTACTGGATATTCTGTACAGAACG
GGGGTAAATTTACTCTGGATTGAAAATGACGGGGGATGTAAAGGTGTATGTAAGCGAATCCCTACAATTAATATTGAGCC
ATCAAATTCAGATAATACATTATGCAAGAAAAACTCATGTTATGATGAGGTTATGCTAAAAAATATTGATGAGTATATAA
TAAATAACTCAGAGGATAAACTTATCGTATTTCATTTGATAGGTAGTCACGGTCCTACCTACTATCTCCGTTATCCTGAG
TCACATAAATATTTTAAACCAACATGTGATCGTAGTGATATCGAAAATTGTACGCATGAGCAATTGATTAATACTTATGA
TAATACAATTCGCTATACTGACTATATAATATCAAAATTAATTGACAAGCTTATCGAATATAAAGATGAGTATGATGCTG
TTTTATTATATGTCTCTGACCATGGAGAATCTCTGGGAGAAAATGGATTATACTTACATGGAACACCATATAATGTTGCA
CCAGCTGAGCAAACGCATGTGCCTCTTATTACCTGGATGTCTCCGGGGTTCGTTTCCTCTAAAAAAATTGATTTGAATTG
TTTGTCAGAACATGCTCTAAACAGAACAGTAAGTCATGATAATATATTTTCATCGTTACTGGGGTATGGAATGTAA

Upstream 100 bases:

>100_bases
TTAAATATGTAAGGGATTTTTTTTTGTTTCTCCTGGCGAGTTTCGTCAGATTGGAACGAATGCATCCCGCACAATAAATG
AAAAACAAAAGCCGGTAATT

Downstream 100 bases:

>100_bases
ATACTTCAGTTTATAATAAAGAAGATGATATTATATCCTCCTGCAGATAGATACTGACATTTTACTGAGGATAAGAGGTG
AATACCTCTTATCCTTAAGT

Product: hypothetical protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSIAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRT
GVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYIINNSEDKLIVFHLIGSHGPTYYLRYPE
SHKYFKPTCDRSDIENCTHEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYDAVLLYVSDHGESLGENGLYLHGTPYNVA
PAEQTHVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGYGM

Sequences:

>Translated_291_residues
MFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSIAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRT
GVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYIINNSEDKLIVFHLIGSHGPTYYLRYPE
SHKYFKPTCDRSDIENCTHEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYDAVLLYVSDHGESLGENGLYLHGTPYNVA
PAEQTHVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGYGM
>Mature_291_residues
MFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSIAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRT
GVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYIINNSEDKLIVFHLIGSHGPTYYLRYPE
SHKYFKPTCDRSDIENCTHEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYDAVLLYVSDHGESLGENGLYLHGTPYNVA
PAEQTHVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGYGM

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=287, Percent_Identity=51.219512195122, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI87082286, Length=263, Percent_Identity=31.5589353612167, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI87082223, Length=258, Percent_Identity=27.1317829457364, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 33156; Mature: 33156

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSIAISVPCMFSDMKRK
CEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCHHHCCCEEEEEECHHHHHHHHH
EFNSRKAVNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSC
HHHHHHCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCEEEECCCCCCCCEECCCCC
YDEVMLKNIDEYIINNSEDKLIVFHLIGSHGPTYYLRYPESHKYFKPTCDRSDIENCTHE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
QLINTYDNTIRYTDYIISKLIDKLIEYKDEYDAVLLYVSDHGESLGENGLYLHGTPYNVA
HHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCC
PAEQTHVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGYGM
CCCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSIAISVPCMFSDMKRK
CEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCHHHCCCEEEEEECHHHHHHHHH
EFNSRKAVNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSC
HHHHHHCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCEEEECCCCCCCCEECCCCC
YDEVMLKNIDEYIINNSEDKLIVFHLIGSHGPTYYLRYPESHKYFKPTCDRSDIENCTHE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
QLINTYDNTIRYTDYIISKLIDKLIEYKDEYDAVLLYVSDHGESLGENGLYLHGTPYNVA
HHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCC
PAEQTHVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGYGM
CCCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]