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The map label for this gene is pilQ [H]

Identifier: 218691158

GI number: 218691158

Start: 3437229

End: 3438749

Strand: Direct

Name: pilQ [H]

Synonym: ECED1_3507

Alternate gene names: 218691158

Gene position: 3437229-3438749 (Clockwise)

Preceding gene: 218691157

Following gene: 218691159

Centisome position: 65.98

GC content: 55.36

Gene sequence:

>1521_bases
ATGCCAGAGACTATCGTACTGCCTCCTGAAGATATTCGTGATTTCGTCGCAATTCAGCATGACGCTGACGGAAAAATCAG
GGTGAAAGTCGTCGAGGCGAAGAAGCACATCACCTCTGTACAACGTTACCTGACGCTCTTACGCTCCCGCTATCCGGGAA
AAATCAGCAAATTCGATTTTACCACTCTTGACGTTATCGCAACCCTGAACAGCAATAATACGGAACGTGCACCAGGCGTT
TTATCCGACGGAGGGATCAACACAGTACAAAGAAAAGTTCTTGATTACATCAGAAAAGCCGCTCAACTGGATGCCTCAGA
TCTGCATATCACACCAGGACGCGACAACACGGACTTCACCTATGTGGAAGCCCGGGTTCACGGCGAACTGGAAGTGCTCG
ATATCCTCCGGAAAGAAGAAGGTCTCGAATTACTGGGGGCCACCTATTCCGGCATGACCGACGTCATCAAGGGCACACAG
TTTGATCCCGGGGTGCCTCAGGATGCGCGTCTTTCAGAGCAGTACCTGAAACTGGCCGGTCTGTTTGGTGCACGCTACTC
CCACTATCCCTGCGTGGGCGGCCTCTATGCTGTGCTCCGTCTGATCAAGGATGACAGTCTGCAAATCCCGACCTTCAGCA
TGCTGGGCTATCACCCTGACCAGGAGCTCACCCTGCGCCGTATTCTGCAGCGCCCTGAAGGTATTATCACGCTCTCAGGA
CCAACAGGTTCAGGAAAATCCACCACGCTGAGAACCGCCTCTGCGGCATACCTTGAACAGTACGGCTTCAACAATACCGG
CGGTATTCTTCTGCCCCGTCGCAGGCTGTTCACCATTGAATCCCCGCCAGAGGGCCGTATACCCGGTGCCATTCAGACTG
CCGTCATGGATTCCGCACAGGGCTGGGTGGACTCCATTAAATCAGCACTGCGTCTTGACCCTGACGGGATCCTGAACGGC
GAAATCCGTGACCACGATTCCGCCATTACGGCAATAAAAGCCGCCATGACCGGGCATCTGATGCTGACCACCATCCATGC
GAACGACCCGATTAACATTCTCGAACGTCTTGAGATGGAGGGGGTACAGGCAAGGATGATTGCCGATCCGCAGCTGTTCA
TTGGCCTGCTGAGCCAGCGTCTGGTTCAGCTGATATGTCCGCACTGCCGTCGCCCCTGGCATGAGGTGGCCACCGACCGC
ACGGATGATGAACGGCGCCTGGTGGAAAGCGTCTGTAACCCGGACCAGGTATATCTGAGAAACCATGAAGGCTGCCCGCA
CTGCTGGCGTGGCGTGACGGGGCGAACGGTGATCGCAGAAGTTATCTCCCCGGATGCGCGTTTTTTCCAGCTGTACCGGG
AAAAGGGGCGTATCGAGGCCAGAACCTACTGGCACCGGGAACTGGGCGGAATGACCCGTAATCAGCATCTGCTGGGAAAA
ATCAACAGCGGACAGGTTGATCCGCTGGCGGCCCATTACATCTCTCCCGTCGATGAGGACAGCTATACGCTGCTGCACTG
A

Upstream 100 bases:

>100_bases
ATTACCCGGTACACTGTTCATGGTGGAACAAATCACACCGGACAGTGTGATTCTGGAATACAACGGCAACAGGCAGACGC
TACGCCCGTGAGGTGCCATC

Downstream 100 bases:

>100_bases
TATGAAATATCTGGAAAAACTCCACAGTACCGACATCGAGCTGACAGCACTGACCGTCTGGATAGCCAGAAAAACCTTTG
CCCTGAACCGGGTGGAGTTC

Product: Type IV pilus (ATPase)

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPETIVLPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYLTLLRSRYPGKISKFDFTTLDVIATLNSNNTERAPGV
LSDGGINTVQRKVLDYIRKAAQLDASDLHITPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQ
FDPGVPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSLQIPTFSMLGYHPDQELTLRRILQRPEGIITLSG
PTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIESPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNG
EIRDHDSAITAIKAAMTGHLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRRPWHEVATDR
TDDERRLVESVCNPDQVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYREKGRIEARTYWHRELGGMTRNQHLLGK
INSGQVDPLAAHYISPVDEDSYTLLH

Sequences:

>Translated_506_residues
MPETIVLPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYLTLLRSRYPGKISKFDFTTLDVIATLNSNNTERAPGV
LSDGGINTVQRKVLDYIRKAAQLDASDLHITPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQ
FDPGVPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSLQIPTFSMLGYHPDQELTLRRILQRPEGIITLSG
PTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIESPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNG
EIRDHDSAITAIKAAMTGHLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRRPWHEVATDR
TDDERRLVESVCNPDQVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYREKGRIEARTYWHRELGGMTRNQHLLGK
INSGQVDPLAAHYISPVDEDSYTLLH
>Mature_505_residues
PETIVLPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYLTLLRSRYPGKISKFDFTTLDVIATLNSNNTERAPGVL
SDGGINTVQRKVLDYIRKAAQLDASDLHITPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQF
DPGVPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSLQIPTFSMLGYHPDQELTLRRILQRPEGIITLSGP
TGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIESPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNGE
IRDHDSAITAIKAAMTGHLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRRPWHEVATDRT
DDERRLVESVCNPDQVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYREKGRIEARTYWHRELGGMTRNQHLLGKI
NSGQVDPLAAHYISPVDEDSYTLLH

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=32.2077922077922, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789723, Length=378, Percent_Identity=31.4814814814815, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082188, Length=177, Percent_Identity=32.2033898305085, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 56586; Mature: 56455

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETIVLPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYLTLLRSRYPGKISKFDF
CCCCEECCCHHHHHHEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TTLDVIATLNSNNTERAPGVLSDGGINTVQRKVLDYIRKAAQLDASDLHITPGRDNTDFT
HHEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEE
YVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPGVPQDARLSEQYLKLAG
EEEEEECCCHHHHHHHHHHCCCCEECCHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LFGARYSHYPCVGGLYAVLRLIKDDSLQIPTFSMLGYHPDQELTLRRILQRPEGIITLSG
HHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHCCCCCHHHHHHHHHHCCCCEEEECC
PTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIESPPEGRIPGAIQTAVMDSAQ
CCCCCCCCEEEHHHHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCCCCHHHHHHHHHHH
GWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTGHLMLTTIHANDPINILERLEME
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHC
GVQARMIADPQLFIGLLSQRLVQLICPHCRRPWHEVATDRTDDERRLVESVCNPDQVYLR
CCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCEEEEE
NHEGCPHCWRGVTGRTVIAEVISPDARFFQLYREKGRIEARTYWHRELGGMTRNQHLLGK
CCCCCCHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCEEEE
INSGQVDPLAAHYISPVDEDSYTLLH
CCCCCCCHHHHHHCCCCCCCCEEECC
>Mature Secondary Structure 
PETIVLPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYLTLLRSRYPGKISKFDF
CCCEECCCHHHHHHEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TTLDVIATLNSNNTERAPGVLSDGGINTVQRKVLDYIRKAAQLDASDLHITPGRDNTDFT
HHEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEE
YVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPGVPQDARLSEQYLKLAG
EEEEEECCCHHHHHHHHHHCCCCEECCHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LFGARYSHYPCVGGLYAVLRLIKDDSLQIPTFSMLGYHPDQELTLRRILQRPEGIITLSG
HHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHCCCCCHHHHHHHHHHCCCCEEEECC
PTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIESPPEGRIPGAIQTAVMDSAQ
CCCCCCCCEEEHHHHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCCCCHHHHHHHHHHH
GWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTGHLMLTTIHANDPINILERLEME
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHC
GVQARMIADPQLFIGLLSQRLVQLICPHCRRPWHEVATDRTDDERRLVESVCNPDQVYLR
CCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCEEEEE
NHEGCPHCWRGVTGRTVIAEVISPDARFFQLYREKGRIEARTYWHRELGGMTRNQHLLGK
CCCCCCHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCEEEE
INSGQVDPLAAHYISPVDEDSYTLLH
CCCCCCCHHHHHHCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]