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The map label for this gene is ygfS [H]

Identifier: 218691011

GI number: 218691011

Start: 3294037

End: 3294525

Strand: Reverse

Name: ygfS [H]

Synonym: ECED1_3345

Alternate gene names: 218691011

Gene position: 3294525-3294037 (Counterclockwise)

Preceding gene: 218691012

Following gene: 218691002

Centisome position: 63.24

GC content: 55.01

Gene sequence:

>489_bases
ATGAAATCGTTAATTATCGTTAATCCGGCTGACTGTATTGGCTGCCGTACTTGTGAAGTGGCCTGTGTGGTCGCTCACCC
TTCAGAACAGGAGTTGAATGCCGATATCTTTTTACCCCGGCTGAAGGTACAACGGCTGGATAGCATCAGTGCGCCGGTGA
TGTGCCATCAGTGTGAAAACGCCCCTTGTGTTGGCGCTTGCCCCGTGGGGGCGCTGACGATGGGCGAGCAGGTGGTGCAG
GCCAATTCTGCCCGTTGTATTGGCTGTCAGAGTTGCGTTAGCACGTGCCCGTTTGGGATGATCACCATTCAGTCATTGCC
GGGTGATCCCCGGCAACAAATCGTGAAATGCGATCTCTGTGAACTGCGGGAAGAGGGGCCAGCCTGCGTTGAATCTTGCC
CGACACAGGCGCTGCAGTTGCTTACTGAAAGAGAACTCAGGCGTATCCGCCAGCAGCGTATTGCTGCCAGCAGTGAGAAT
CCTCTCTGA

Upstream 100 bases:

>100_bases
GTGATGCAGTTCATGGCGCGGATCTGGTTGTCACTGCAATGGCCGCAGGAAGGCAGGCGGCGCGCGATATGTTAACTCTG
TTTGATACGAAGGCATCGTG

Downstream 100 bases:

>100_bases
AAGAAATCTCCGCGCGGAAAGGCGCGGAGAATCTATTAGATTGCCCAACCACCCGCGTAAAACGCGACCAGTGCGGCAGT
AATGATCACCGTACCAATAT

Product: putative oxidoreductase, 4Fe-4S ferredoxin-type subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQRLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQ
ANSARCIGCQSCVSTCPFGMITIQSLPGDPRQQIVKCDLCELREEGPACVESCPTQALQLLTERELRRIRQQRIAASSEN
PL

Sequences:

>Translated_162_residues
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQRLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQ
ANSARCIGCQSCVSTCPFGMITIQSLPGDPRQQIVKCDLCELREEGPACVESCPTQALQLLTERELRRIRQQRIAASSEN
PL
>Mature_162_residues
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQRLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQ
ANSARCIGCQSCVSTCPFGMITIQSLPGDPRQQIVKCDLCELREEGPACVESCPTQALQLLTERELRRIRQQRIAASSEN
PL

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082179, Length=162, Percent_Identity=95.0617283950617, Blast_Score=309, Evalue=5e-86,
Organism=Escherichia coli, GI1788811, Length=166, Percent_Identity=43.9759036144578, Blast_Score=138, Evalue=2e-34,
Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=44.5859872611465, Blast_Score=135, Evalue=1e-33,
Organism=Escherichia coli, GI1789067, Length=167, Percent_Identity=39.5209580838323, Blast_Score=127, Evalue=3e-31,
Organism=Escherichia coli, GI87082180, Length=163, Percent_Identity=44.7852760736196, Blast_Score=127, Evalue=3e-31,
Organism=Escherichia coli, GI87082114, Length=202, Percent_Identity=36.6336633663366, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1789079, Length=183, Percent_Identity=34.9726775956284, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787749, Length=173, Percent_Identity=29.4797687861272, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1790326, Length=173, Percent_Identity=28.3236994219653, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787122, Length=162, Percent_Identity=30.2469135802469, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787963, Length=163, Percent_Identity=26.3803680981595, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=29.6296296296296, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI2367345, Length=138, Percent_Identity=30.4347826086957, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17525; Mature: 17525

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
12.3 %Cys+Met (Translated Protein)
9.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
12.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQRLDSISAPVMCHQCEN
CCCEEEECCHHHCCCCCEEEEEEEECCCCHHCCCEEECCHHHHHHHHCCCCCEEEECCCC
APCVGACPVGALTMGEQVVQANSARCIGCQSCVSTCPFGMITIQSLPGDPRQQIVKCDLC
CCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
ELREEGPACVESCPTQALQLLTERELRRIRQQRIAASSENPL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQRLDSISAPVMCHQCEN
CCCEEEECCHHHCCCCCEEEEEEEECCCCHHCCCEEECCHHHHHHHHCCCCCEEEECCCC
APCVGACPVGALTMGEQVVQANSARCIGCQSCVSTCPFGMITIQSLPGDPRQQIVKCDLC
CCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
ELREEGPACVESCPTQALQLLTERELRRIRQQRIAASSENPL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]