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The map label for this gene is int [H]

Identifier: 218690990

GI number: 218690990

Start: 3263297

End: 3264550

Strand: Reverse

Name: int [H]

Synonym: ECED1_3324

Alternate gene names: 218690990

Gene position: 3264550-3263297 (Counterclockwise)

Preceding gene: 218690991

Following gene: 218690987

Centisome position: 62.66

GC content: 50.08

Gene sequence:

>1254_bases
ATGCGTATAGGACAAAATCGTTTTTTGGGGGCTATACACATGTTGCTTTCAGACCTTGCTATCCGCCGCGCGAAGCCAAA
AGAGAAAGCCTATACACTCAATGACGGCAACGGGCTTTCGTTACTCATTGAGCCGAACGGTTCGAAGGGCTGGCGATTGC
GATACAGGTTTGCAGGCAAGCCCAAAATGTTGTCGCTGGGTGTCTATCCTGAGGTGTCCCTTTCTGATGCCAGGACGGCA
AGGGATGAAGCCAAAAGGCTTATCGCTGGTGGAGTTAATCCGAGTGAGGTCAGAAAGGCCCAAAAGCGAGAACAGGCGAA
CAAATGCGGTAACACCTTTGAGGTGATCGCCCGTGAGTGGTATGAAAAACGCCTGGACCGATGGTCTCCCTCATATGCAG
AGGAAATGATAGAGACGTTTGAAAAAGACGTTTTTCCGTACATTGGAGGTAGGCCCATCGCAGAGATTAAGCCGATGGAA
TTGCTCGCGGTTCTATCCCGTCTTAATGACCGTGGCGCAACTGAAAAATTGCGTAAGGTTCGCCAGCGATGTGGCGAAGT
GTTTCGGTACGCAATAATTACCGGACGCGCTGAATATAACCCCGCTCCTGATCTGGCAAGCGCATTTGCACCACACAAAA
AAGAGCATTACGCGTATCTGAAAGTTGATGAGCTACCGGAATTTTTCCGCGTTTTTAATACGTACACCGGAAGCCAGATC
GTCAAGCTGGGTATGCGATTGTTGATTCTTACAGGACTCCGTCCGGGTGAATTGCGGCAGGCAGAGTGGCAGGAGATTGA
TTTTGATAATCGTCTGTGGGAAGTGCCGAAAGAACGAATGAAAATGCGCCGTCCTCATTGCGTGCCGCTTTCAGAACAGG
CGATTGCTATTCTGGAACAACTGAAACACATGACGGGGCAGTATCCGTTTATTTTCCCTGGTCGAATACAGCACAGTAAA
CCGATGAGTGAAATGGCGATGAACGTGCTTATTCGTCGTATTGGCTATGCTGGGCGCGTTACTGGTCATGGGTTCCGGCA
TACCATGAGTACAATTTTGCATGAGCAGGGTTATAACTCCGCGTGGATAGAAACGCAGCTGGCGCACGTCGATAAAAACT
CGATCCGTGGCACATACAATCACGCGCAATATCTGGACGGACGGCGGGAGATGCTCCAGTGGTACGCCGACTATATGGAC
TCACTGGAACAGGGCGGAAACGTGGTGCACGGCAAGTTTAGTGTATGTGCATAA

Upstream 100 bases:

>100_bases
AAGTCTACCAATCCCCCGAATATCAAGGCCTCCAGCGTTTTTCCTCGTCTACAACCGTCTACAGTCATCTACCTAAATCT
ACACACAGAAGTGTATAGTA

Downstream 100 bases:

>100_bases
AAACGACTGGACAAGTATACAGATGTATGCTAACGTACACGGAAAGCTACAGCTAACATCCTTACAGAGGGTTTTTCACG
CAGTTATCCGTCATGTATGG

Product: Integrase from prophage

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MRIGQNRFLGAIHMLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRFAGKPKMLSLGVYPEVSLSDARTA
RDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEVIAREWYEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPME
LLAVLSRLNDRGATEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRVFNTYTGSQI
VKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVPLSEQAIAILEQLKHMTGQYPFIFPGRIQHSK
PMSEMAMNVLIRRIGYAGRVTGHGFRHTMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMD
SLEQGGNVVHGKFSVCA

Sequences:

>Translated_417_residues
MRIGQNRFLGAIHMLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRFAGKPKMLSLGVYPEVSLSDARTA
RDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEVIAREWYEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPME
LLAVLSRLNDRGATEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRVFNTYTGSQI
VKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVPLSEQAIAILEQLKHMTGQYPFIFPGRIQHSK
PMSEMAMNVLIRRIGYAGRVTGHGFRHTMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMD
SLEQGGNVVHGKFSVCA
>Mature_417_residues
MRIGQNRFLGAIHMLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRFAGKPKMLSLGVYPEVSLSDARTA
RDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEVIAREWYEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPME
LLAVLSRLNDRGATEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRVFNTYTGSQI
VKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVPLSEQAIAILEQLKHMTGQYPFIFPGRIQHSK
PMSEMAMNVLIRRIGYAGRVTGHGFRHTMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMD
SLEQGGNVVHGKFSVCA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=387, Percent_Identity=44.9612403100775, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI145693166, Length=396, Percent_Identity=42.4242424242424, Blast_Score=307, Evalue=6e-85,
Organism=Escherichia coli, GI1788974, Length=396, Percent_Identity=39.3939393939394, Blast_Score=302, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 48022; Mature: 48022

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGQNRFLGAIHMLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRFAGK
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCCEEEEEEECCC
PKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEVIAREW
CCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
YEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPMELLAVLSRLNDRGATEKLRKV
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
RQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRVFNTYTGSQI
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHH
VKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVPLSEQAIAILEQ
HHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHH
LKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRHTMSTILHEQGYNS
HHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
AWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQGGNVVHGKFSVCA
HHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEECC
>Mature Secondary Structure
MRIGQNRFLGAIHMLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRFAGK
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCCEEEEEEECCC
PKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEVIAREW
CCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
YEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPMELLAVLSRLNDRGATEKLRKV
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
RQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRVFNTYTGSQI
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHH
VKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVPLSEQAIAILEQ
HHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHH
LKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRHTMSTILHEQGYNS
HHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
AWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQGGNVVHGKFSVCA
HHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]